scholarly journals Amino acid stimulation of carbon dioxide fixation in cell-free extracts of Chlamydomonas reinhardtii.

1970 ◽  
Vol 46 (5) ◽  
pp. 761-762 ◽  
Author(s):  
R F Jones ◽  
J H Chen
Keyword(s):  
Carbon Dioxide ◽  
Amino Acid ◽  
Chlamydomonas Reinhardtii ◽  
Carbon Dioxide Fixation ◽  
Stimulation Of

Effects of adenosine triphosphate and inorganic pyrophosphate on carbon dioxide fixation by isolated spinach chloroplasts

1969 ◽  
Vol 47 (4) ◽  
pp. 443-447 ◽  
Author(s):  
John R. Vose ◽  
Mary Spencer
Keyword(s):  
Carbon Dioxide ◽  
Adenosine Triphosphate ◽  
Carbon Dioxide Fixation ◽  
Enhancement Effect ◽  
Inorganic Pyrophosphate ◽  
Spinach Chloroplasts ◽  
Stimulation Of

The effects of exogenous ATP and inorganic pyrophosphate on CO2 fixation were investigated with preparations of intact spinach chloroplasts. Under illumination, both ATP and inorganic pyrophosphate resulted in an increase in the rate of CO2 fixation. These stimuli were generally equivalent to one another, and the degree of stimulation was dependent upon the age and condition of the chloroplasts used. During a reaction the enhancement effect increased with time; the initial kinetics (72 μmoles CO2 fixed per milligram chlorophyll per hour) were unaffected by either ATP or inorganic pyrophosphate. The stimulation of CO2 fixation by ATP or inorganic pyrophosphate was inhibited by a buffer pH of more than 8.0. Concentrations of exogenous magnesium above 0.25 mM were found to inhibit both the ATP- and inorganic pyrophosphate-induced increases in CO2 fixation. Phloridzin was found to partially inhibit the enhancement of CO2 fixation by ATP and inorganic pyrophosphate.

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