scholarly journals Limitation of Tobacco Callus Tissue Growth by Carbohydrate Availability

1970 ◽  
Vol 46 (1) ◽  
pp. 118-122 ◽  
Author(s):  
C. D. Upper ◽  
John P. Helgeson ◽  
G. T. Haberlach
Keyword(s):  
Callus Tissue ◽  
Tissue Growth ◽  
Tobacco Callus ◽  
Carbohydrate Availability

scholarly journals The effect of benzene and its Chlorella vulgaris metabolites on the growth of tobacco tissue

2014 ◽  
Vol 64 (4) ◽  
pp. 366-370
Author(s):  
E. Szczepkowska-Kuźnicka ◽  
W. Wardas
Keyword(s):  
Nicotiana Tabacum ◽  
Toxic Effect ◽  
Chlorella Vulgaris ◽  
Microscopic Examination ◽  
Callus Tissue ◽  
Tissue Growth ◽  
Tobacco Callus ◽  
Cultivation Medium

The influence of benzene, phenol and catechol on the tobacco callus tissue (<i>Nicotiana tabacum</i> L. cv. Wisconsin No. 38) cultivated <i>in vitro</i> was studied. Although inhibition of callus tissue growth in relation to the control was observed in all variants, the benzene-induced inhibition was the most significant. When catechol and phenol were added to the cultivation medium together, callus tissue growth was similar to that of the control. The addition of catechol to the medium containing phenol presumably reduces the toxic effect of phenol on the callus tissue examined. Callus tissue growing in the presence of phenol, benzene and cathechol became senescent more rapidly than in the control. Moreover microscopic examination revealed significant changes in the callus structure.

Growth Inhibition in Tobacco (Nicotiana tabacum) Callus by 2,6-Dinitroaniline Herbicides and Protection by D-α-tocopherol Acetate

Weed Science ◽  
1978 ◽  
Vol 26 (6) ◽  
pp. 527-530 ◽  
Author(s):  
J. B. Huffman ◽  
N. D. Camper
Keyword(s):  
Regression Analysis ◽  
Nicotiana Tabacum ◽  
Callus Tissue ◽  
Linear Regression Analysis ◽  
Tissue Growth ◽  
Tissue Cultures ◽  
Fresh Weight ◽  
Structural Variations ◽  
Tocopherol Acetate ◽  
Dinitroaniline Herbicides

Tobacco(Nicotiana tabacumL. ‘X-73’) callus tissue cultures were used in a bioassay system for determining the effect of the following substituted 2,6-dinitroaniline herbicides on growth: trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine); oryzalin (3,5-dinitro-N4,N4-dipropylsulfanilamide); benefin(N-butyl-N-ethyl-α,α,α-trifluoro-2,6-dinitro-p-toluidine); AC 92390(N-sec-butyl-2,6-dinitro-3,4-xylidine); penoxalin [N-(1-ethylpropyl)-2,6-dinitro-3,4-xylidine]; GS-38946(N-ethyl-N-tetrahydrofurfuryl-4-trifluoromethyl-2,6-dinitroaniline); chlornidine [N,N-di(2-chloroethyl)-4-methyl-2,6-dinitroaniline]; nitralin [4-(methylsulfonyl)2,6-dinitro-N,N-dipropylaniline]; dinitramine(N4,N4-diethyl-α,α,α-trifluoro-3,5-dinitrotoluene-2,4-diamine); isopropalin (2,6-dinitro-N,N-dipropylcumidine), and profluralin [N(cyclopropylmethyl)-α,α,α-trifluoro-2,6-dinitro-N-propyl-p-toluidine]. The molar concentration required to inhibit fresh weight gain by 50% (I50) was determined by using linear regression analysis on data from a range of five concentrations of each chemical. I50values did not correlate with structural variations or available physical constants. Herbicides listed in order of increasing activity are AC 92390< GD-38946<profluralin = isopropalin<benefin = chlornidine = trifluralin = nitralin<oryzalin = penoxalin < dinitramine. Exogenously applied D-α-tocopherol acetate at 100 times the I50concentrations decreased the inhibition of tissue growth by the herbicides.

Uptake of Sugars by Tobacco Callus Tissue

1973 ◽  
Vol 15 (5) ◽  
pp. 312-317 ◽  
Author(s):  
Miroslava Opekaeová ◽  
A. Kotyk
Keyword(s):  
Callus Tissue ◽  
Tobacco Callus

Growth substance requirements and major lipid constituents of tissue cultures of Euphorbia esula and E. cyparissias

1972 ◽  
Vol 50 (4) ◽  
pp. 723-726 ◽  
Author(s):  
T. T. Lee ◽  
A. N. Starratt
Keyword(s):  
Callus Tissue ◽  
Defined Medium ◽  
Growth Substance ◽  
Tissue Growth ◽  
Euphorbia Esula ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Callus Tissues ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Root Tissues

The root tissues of Euphorbia esula and E. cyparissias form callus on chemically defined medium. Both species require an exogenous supply of auxin for growth, but the appearance and color of the tissue and their responses to kinetin, 2,4-dichlorophenoxyacetic acid (2,4-D) and indoleacetic acid (IAA) are different. The tissue growth is more satisfactory with α-naphthaleneacetic acid (NAA) than with 2,4-D, IAA, or 4-amino-3,5,6-trichloropicolinic acid (picloram). Gibberellic acid has no effect. The callus tissues of E. esula become intensely green under light but are not autotrophic.Triglycerides, palmitic acid, and β-sitosterol are the major lipid constituents of the callus tissue of E. esula. Chromatographic analysis reveals no significant differences in the composition of extracts from the non-green and green tissues. Long-chain aldehydes, alcohols, and triterpenes found in the plant are not detected in the cultures.

scholarly journals Physiological and biochemical effects of morphactin IT 3233 on callus and tumour tissues of Nicotiana tabacum L. cultured in vitro. IV. IAA oxidase activity. Oat coleoptile biotest

2015 ◽  
Vol 43 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Z. Chirek
Keyword(s):  
Oxidase Activity ◽  
Callus Tissue ◽  
Tissue Growth ◽  
Tumour Tissue ◽  
Iaa Oxidase ◽  
Nicotiana Tabacum L ◽  
Coleoptile Elongation ◽  
Short Time ◽  
Physiological And Biochemical

IAA oxidase activity in callus and tumour tissue of tobacco subjected to the action of morphactin IT 3233 for shorter and longer periods was determined. Control tumour tissue shows an activity higher by about 40 per cent as compared with that of callus tissue. Morphactin applied for a short time (24-h incubation) does not change the activity of the enzyme. When application is prolonged, a considerable enhancement (up to 140%) of the enzyme activity in callus tissue is observed in dependence on the morphactin concentration. In tumour tissues the activity is stimulated by 45 per cent as compared to control. Oat coleoptile elongation growth induced by IAA is limited to 40 per cent when morphactin is added in the concentrations used for tobacco tissue cultures. The possibility of the morphactin action on tissue growth via IAA metabolism is discussed.

scholarly journals on the Fate of Tyrosine in Tobacco Callus Tissue II. The Lack of Incorporation Into Lignin

1962 ◽  
Vol 15 (4) ◽  
pp. 619 ◽  
Author(s):  
DK Dougall
Keyword(s):  
Callus Tissue ◽  
Insoluble Fraction ◽  
Tobacco Callus

The presence of lignin in an insoluble fraction obtained from tobacco callus tissue was shown by the production of vanillin and syringaldehyde on oxidation of the material with nitrobenzene in alkali.

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