scholarly journals Studies on Cytokinin-Controlled Bud Formation in Moss Protonemata

1968 ◽  
Vol 43 (5) ◽  
pp. 827-837 ◽  
Author(s):  
H. Brandes ◽  
H. Kende
HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 508b-508
Author(s):  
Pamela M. Lewis ◽  
Alan M. Armitage ◽  
Jim M. Garner

The effect of vernalization method and duration on off-season cut flower production of Lysimachia clethroides Duby was examined. Rhizomes harvested in October were cooled for 0, 4, 6, 8, 10 or 12 weeks at 4 ± 1 °C in crates with unmilled sphagnum peat or in 3.75-L pots with potting media prior to forcing in a warm greenhouse. After 6 or more weeks of cooling, shoots emerged from crate-cooled rhizomes in higher percentages than from pot-cooled rhizomes. However, only the duration of cooling, not the method, affected the rate of shoot emergence, visible bud formation and anthesis of the first bud in the raceme. As cooling increased from 0 to 12 weeks, the greenhouse days required for shoot emergence, visible bud formation and anthesis decreased linearly. The number of flowering flushes and flowering stems varied quadratically with cooling duration, and the highest yields occurred when rhizomes received between 4 and 10 weeks of cooling. As the number of successive flowering flushes increased, the stem length increased linearly while the stem diameter decreased linearly. High numbers of flowers were produced rapidly after 10 weeks of cooling.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 478e-479
Author(s):  
M.M. Jenderek ◽  
A.J. Olney

Hibiscus syriacus is a difficult species in micropropagation due to its endogenous contamination and recalcitrant shoot formation; therefore, studies on using explants other than shoot tip or axillary buds of growing shrubs were initiated. Three different seedling fragments (root, hypocotyl, and leaf petiole) from aseptically germinated seedlings of hibiscus (var. Aphrodite) were evaluated for adventitious bud formation, shoot and leaf development. The explants were cultured on McCown's woody plant basal salt medium supplemented with KNO3 (800 mg/L), adenine sulfate (80 mg/L) and MS vitamins containing BA or 2iP or TDZ at 0.5, 1.0, 2.2, 4.4 and 10 mM. Adventitious buds were present on all of the three different explants grown on medium containing TDZ; however, the most abundant bud formation, with many small leaves originating from callus was observed on hypocotyl explants cultured on medium with 1 mM of TDZ. Petiole explants were the most frequent to develop short shoots (≈15 mm) and one to nine leaves without callus formation, where 70% of hypocotyl and the root explants formed leaves originating from callus. Callus was induced on all explant types regardless of the level or type of cytokinin used. However, the number of shoots produced by any explant type was low, petioles cultured on 0.5 and 1mM of TDZ were the most suitable material for non-callus shoot development in H. syriacus. Hypocotyl explants proved to be an excellent source for adventitious bud formation but their ability to develop shoots needs to be investigated.


1987 ◽  
Vol 70 (3) ◽  
pp. 453-460 ◽  
Author(s):  
Patrizia Torrigiani ◽  
Maria Maddalena Altamura ◽  
Gabriella Pasqua ◽  
Barbara Monacelli ◽  
Donatella Serafini-Fracassini ◽  
...  

2001 ◽  
Vol 114 (1) ◽  
pp. 207-218 ◽  
Author(s):  
C.G. Padmashree ◽  
U. Surana

In the budding yeast Saccharomyces cerevisiae, a prospective mother normally commences the formation of a daughter (the bud) only in the G(1) phase of the cell division cycle. This suggests a strict temporal regulation of the processes that initiate the formation of a new bud. Using cortical localization of bud site components Spa2 and Bni1 as an indicator of bud site assembly, we show that cells assemble a bud site following inactivation of the Cdc28-Clb mitotic kinase but prior to START. Interestingly, an untimely inactivation of the mitotic kinase is sufficient to drive cells to assemble a new bud site inappropriately in G(2) or M phases. The induction of Cdc28/Clb kinase activity in G(1), on the other hand, dramatically reduces a cell's ability to construct an incipient bud site. Our findings strongly suggest that the Cdc28-Clb kinase plays a critical role in the mechanism that restricts the timing of bud formation to the G(1) phase of the cell cycle.


2017 ◽  
Vol 216 (3) ◽  
pp. 829-840 ◽  
Author(s):  
Jiqing Gou ◽  
Chunxiang Fu ◽  
Sijia Liu ◽  
Chaorong Tang ◽  
Smriti Debnath ◽  
...  

1972 ◽  
Vol 25 (4) ◽  
pp. 691 ◽  
Author(s):  
NP Kefford ◽  
OH Caso

The effects of endogenous factors (plant age, section length, and section location) and environmental factors (temperature and mineral nutrition) upon organ regeneration on isolated root sections of Ohondrilla juncea L. were used to develop a standard assay system for the study of the chemical regulation of regeneration. Bud and root formation and its polarity in the presence of a variety of regulators alone and in combinations were observed quantitatively. Bud numbers were increased by auxin (low concentrations), cytokinin, and gibberellin treatments. High concentra� tions of auxin inhibited bud formation and this effect was reversed by antiauxin, cytokinin, or gibberellin. Adenine did not counteract auxin� induced bud inhibition but adenine and N�6�benzyladenine did counteract inhibition induced by the purine antagonist 2,6�diaminopurine. Numbers of regenerated roots were increased by auxin treatment and reduced by cytokinin and gibberellin treatment. On control and auxin� treated sections, bud formation was strongly polar and proximal and cytokinin and gibberellin treatments lessened the polarity. Growth retardants inhibited regeneration. Of a number of synthetic auxins tested, 2,4.dichlorophenoxy. acet.O.methylhydroxamic acid and 4�amino.3,5,6.trichloropicolinic acid were the most effective inhibitors of bud formation.


1961 ◽  
Vol 12 (1) ◽  
pp. 69 ◽  
Author(s):  
AJ Antcliff ◽  
WJ Webster ◽  
P May

An experiment comparing crop regulation by pruning with crop regulation by disbunching is described. For three out of four seasons both methods of regulation gave similar yields, and only in one season did disbunched vines yield somewhat better than more severely pruned vines with the same number of bunches. This better yield appeared to be due to larger berries on the disbunched vines. Increased vegetative growth on the disbunched vines was not accompanied by improved fruit bud formation or by better bud burst in the following season. It is concluded that the leaf/fruit ratio has little effect on the yield of sultanas under Murray Valley conditions, and that methods of crop forecasting and regulation by pruning developed for this region should not be affected by the variation in leaf / fruit ratio to which they give rise.


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