scholarly journals Nitrogen and Amino Acids in Normal, Habituated, and Bacteria-Free Crown Gall Tumor Tissue Cultures of Grape

1952 ◽  
Vol 27 (1) ◽  
pp. 173-178 ◽  
Author(s):  
Addison E. Lee
Keyword(s):  
Amino Acids ◽  
Crown Gall ◽  
Tumor Tissue ◽  
Tissue Cultures ◽  
Crown Gall Tumor

scholarly journals IONIC EFFECTS ON LIGNIFICATION AND PEROXIDASE IN TISSUE CULTURES

1965 ◽  
Vol 25 (1) ◽  
pp. 109-116 ◽  
Author(s):  
Jacques Lipetz ◽  
Anthony J. Garro
Keyword(s):  
Cell Wall ◽  
Crown Gall ◽  
Cell Walls ◽  
Tumor Tissue ◽  
Divalent Cations ◽  
Tissue Cultures ◽  
Crown Gall Tumor ◽  
Washed Cell ◽  
Calcium Magnesium ◽  
Ionic Effects

Crown-gall tumor tissue cultures release peroxidase into the medium in response to the concentration of specific ions in the medium. This release is not due to diffusion from cut surfaces or injured cells. Calcium, magnesium, and ammonium were, in that order, most effective in increasing peroxidase release. The enzyme was demonstrated cytochemically on the cell walls and in the cytoplasm. Cell wall fractions, exhaustively washed in buffer, still contained bound peroxidase. This bound peroxidase could be released by treating the wall fractions with certain divalent cations or ammonium. The order of effectiveness for removing the enzyme from the washed cell walls is: Ca++ ≈ Sr++ > Ba++ > Mg++ > NH4+. These data support the thesis presented that specific ions can control the deposition of lignin on cell walls by affecting the peroxidase levels on these walls.

N6-(Δ2-isopentenyl)adenosine from crown gall tumor tissue of Vinca rosea

1976 ◽  
Vol 15 (10) ◽  
pp. 1565 ◽  
Author(s):  
Chong-Maw Chen ◽  
Richard L. Eckert ◽  
James D. McChesney
Keyword(s):  
Crown Gall ◽  
Tumor Tissue ◽  
Crown Gall Tumor ◽  
Vinca Rosea ◽  
Isopentenyl Adenosine

Two octopine dehydrogenases in crown-gall tumor tissue

1977 ◽  
Vol 16 (6) ◽  
pp. 647-650 ◽  
Author(s):  
Paul R. Birnberg ◽  
Barbara B. Lippincott ◽  
James A. Lippincott
Keyword(s):  
Crown Gall ◽  
Tumor Tissue ◽  
Crown Gall Tumor

LYSOPINE IN NORMAL AND IN CROWN-GALL TUMOR TISSUE OF TOMATO AND TOBACCO

1964 ◽  
Vol 42 (8) ◽  
pp. 999-1004 ◽  
Author(s):  
E. W. Seitz ◽  
R. M. Hochster
Keyword(s):  
Quantitative Analysis ◽  
Amino Acid ◽  
Column Chromatography ◽  
Crown Gall ◽  
Normal Tissue ◽  
Tumor Tissue ◽  
Free State ◽  
Crown Gall Tumor

The amino acid lysopine was found in the free state in both primary normal and crown-gall tumor tissue of growing tobacco (Nicotianatabacum) and tomato (Lycopersiconesculentum) plants. Identification was made by means of co-chromatography with synthesized lysopine, electrophoresis, and column chromatography. Quantitative analysis showed that tumor tissue contained about 24 times as much lysopine as normal tissue.

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