scholarly journals STOMATAL MOVEMENT AND PHOTOSYNTHESIS IN PELARGONIUM. II. EFFECTS OF WATER DEFICIT AND OF CHLOROFORM: PHOTOSYNTHESIS IN GUARD CELLS

1951 ◽  
Vol 26 (3) ◽  
pp. 581-597 ◽  
Author(s):  
George W. Scarth ◽  
Michael Shaw
Keyword(s):  
Water Deficit ◽  
Guard Cells ◽  
Stomatal Movement

Crosstalk of NO with Ca2+ in Stomatal Movement in Vicia faba Guard Cells

2009 ◽  
Vol 35 (8) ◽  
pp. 1491-1499 ◽  
Author(s):  
Lin ZHANG ◽  
Xiang ZHAO ◽  
Ya-Jing WANG ◽  
Xiao ZHANG
Keyword(s):  
Vicia Faba ◽  
Guard Cells ◽  
Stomatal Movement

scholarly journals Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells

2019 ◽  
Vol 20 (11) ◽  
pp. 2753
Author(s):  
Xin Li ◽  
Min Diao ◽  
Yanan Zhang ◽  
Guanlin Chen ◽  
Shanjin Huang ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Dynamic Behavior ◽  
Guard Cell ◽  
Actin Filaments ◽  
De Novo ◽  
Guard Cells ◽  
Stomatal Movement ◽  
Selection Of

The actin cytoskeleton is involved in regulating stomatal movement, which forms distinct actin arrays within guard cells of stomata with different apertures. How those actin arrays are formed and maintained remains largely unexplored. Elucidation of the dynamic behavior of differently oriented actin filaments in guard cells will enhance our understanding in this regard. Here, we initially developed a program called ‘guard cell microfilament analyzer’ (GCMA) that enables the selection of individual actin filaments and analysis of their orientations semiautomatically in guard cells. We next traced the dynamics of individual actin filaments and performed careful quantification in open and closed stomata. We found that de novo nucleation of actin filaments occurs at both dorsal and ventral sides of guard cells from open and closed stomata. Interestingly, most of the nucleated actin filaments elongate radially and longitudinally in open and closed stomata, respectively. Strikingly, radial filaments tend to form bundles whereas longitudinal filaments tend to be removed by severing and depolymerization in open stomata. By contrast, longitudinal filaments tend to form bundles that are severed less frequently in closed stomata. These observations provide insights into the formation and maintenance of distinct actin arrays in guard cells in stomata of different apertures.

scholarly journals Downregulating VAC14 in Guard Cells Causes Drought Hypersensitivity by Inhibiting Stomatal Closure

2020 ◽  
Vol 11 ◽  
Author(s):  
Zong-Qi Wang ◽  
Qi Liu ◽  
Ju-Hua Wu ◽  
Juan Li ◽  
Jun-Min He ◽  
...  
Keyword(s):  
Stomatal Closure ◽  
Guard Cells ◽  
Land Plant ◽  
Scaffolding Protein ◽  
Stomatal Movement ◽  
Wild Type ◽  
Physiological Factors ◽  
Surrounding Environment ◽  
Intracellular Activities

Stomata are a key land plant innovation that permit the regulation of gaseous exchanges between the plant interior and the surrounding environment. By opening or closing, stomata regulate transpiration of water though the plant; and these actions are coordinated with acquisition of CO2 for photosynthesis. Stomatal movement is controlled by various environmental and physiological factors and associates with multiple intracellular activities, among which the dynamic remodeling of vacuoles plays a crucial role. Phosphatidylinositol 3,5-bisphosphate [PI(3,5)P2] is critical for dynamic remodeling of vacuoles. Its production requires a PI(3,5)P2-metabolizing complex consisting of FAB1/PIKfyve kinases, SAC phosphatases, and the scaffolding protein VAC14. Although genetic or pharmacological downregulation of PI(3,5)P2 causes hyposensitivity to ABA-induced stomatal closure, whether the effect of PI(3,5)P2 on stomatal movement is cell-autonomous and the physiological consequences of its reduction were unclear. We report that downregulating Arabidopsis VAC14 specifically in guard cells by artificial microRNAs (amiR-VAC14) results in enlarged guard cells and hyposensitivity to ABA- and dark-induced stomatal closure. Vacuolar fission during stomatal closure is compromised by downregulating VAC14 in guard cells. Exogenous application of PI(3,5)P2 rescued the amiR-VAC14 phenotype whereas PI(3,5)P2 inhibitor YM201636 caused wild-type plants to have inhibited stomatal closure. We further show that downregulating VAC14 specifically in guard cells impairs drought tolerance, suggestive of a key role of guard cell-produced PI(3,5)P2 in plant fitness.

scholarly journals Identification of Genes Preferentially Expressed in Stomatal Guard Cells of Arabidopsis thaliana and Involvement of the Aluminum-Activated Malate Transporter 6 Vacuolar Malate Channel in Stomatal Opening

2021 ◽  
Vol 12 ◽  
Author(s):  
Wenxiu Ye ◽  
Shota Koya ◽  
Yuki Hayashi ◽  
Huimin Jiang ◽  
Takaya Oishi ◽  
...  
Keyword(s):  
Guard Cells ◽  
Stomatal Opening ◽  
Stomatal Movement ◽  
Null Mutant ◽  
Reverse Transcription Pcr ◽  
Public Resources ◽  
Stomatal Guard Cells ◽  
Signaling Components ◽  
Malate Transporter ◽  
Gdsl Lipase

Stomatal guard cells (GCs) are highly specialized cells that respond to various stimuli, such as blue light (BL) and abscisic acid, for the regulation of stomatal aperture. Many signaling components that are involved in the stomatal movement are preferentially expressed in GCs. In this study, we identified four new such genes in addition to an aluminum-activated malate transporter, ALMT6, and GDSL lipase, Occlusion of Stomatal Pore 1 (OSP1), based on the expression analysis using public resources, reverse transcription PCR, and promoter-driven β-glucuronidase assays. Some null mutants of GC-specific genes evidenced altered stomatal movement. We further investigated the role played by ALMT6, a vacuolar malate channel, in stomatal opening. Epidermal strips from an ALMT6-null mutant exhibited defective stomatal opening induced by BL and fusicoccin, a strong plasma membrane H+-ATPase activator. The deficiency was enhanced when the assay buffer [Cl–] was low, suggesting that malate and/or Cl– facilitate efficient opening. The results indicate that the GC-specific genes are frequently involved in stomatal movement. Further detailed analyses of the hitherto uncharacterized GC-specific genes will provide new insights into stomatal regulation.

scholarly journals The K+channel KZM2 is involved in stomatal movement by modulating inward K+currents in maize guard cells

2017 ◽  
Vol 92 (4) ◽  
pp. 662-675 ◽  
Author(s):  
Yong-Qiang Gao ◽  
Wei-Hua Wu ◽  
Yi Wang
Keyword(s):  
Guard Cells ◽  
K Channel ◽  
Stomatal Movement ◽  
K Currents

scholarly journals Actin Filaments in Mature Guard Cells Are Radially Distributed and Involved in Stomatal Movement

1995 ◽  
Vol 109 (3) ◽  
pp. 1077-1084 ◽  
Author(s):  
M. Kim ◽  
P. K. Hepler ◽  
S. O. Eun ◽  
K. S. Ha ◽  
Y. Lee
Keyword(s):  
Actin Filaments ◽  
Guard Cells ◽  
Stomatal Movement
Sign in / Sign up

Export Citation Format

Share Document