scholarly journals Regulation of a Cytochrome P450 Gene CYP94B1 by WRKY33 Transcription Factor Controls Apoplastic Barrier Formation in Roots to Confer Salt Tolerance

2020 ◽  
Vol 184 (4) ◽  
pp. 2199-2215
Author(s):  
Pannaga Krishnamurthy ◽  
Bhushan Vishal ◽  
Wan Jing Ho ◽  
Felicia Chien Joo Lok ◽  
Felicia Si Min Lee ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cytochrome P450 ◽  
Salt Tolerance ◽  
Cytochrome P450 Gene ◽  
P450 Gene ◽  
Barrier Formation ◽  
Apoplastic Barrier

scholarly journals Regulation of CYP94B1 by WRKY33 controls apoplastic barrier formation in the roots leading to salt tolerance

2020 ◽  
Author(s):  
Pannaga Krishnamurthy ◽  
Bhushan Vishal ◽  
Wan Jing Ho ◽  
Felicia Chien Joo Lok ◽  
Felicia Si Min Lee ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Chromatin Immunoprecipitation ◽  
Histochemical Staining ◽  
P450 Gene ◽  
Upstream Regulator ◽  
Barrier Formation ◽  
Apoplastic Barrier ◽  
Plant Salt Tolerance ◽  
Endodermal Cells

AbstractSalinity is an environmental stress that causes decline in crop yield. Avicennia officinalis and other mangroves have adaptations such as ultrafiltration at the roots aided by apoplastic cell-wall barriers to thrive in saline conditions. We studied a Cytochrome P450 gene, AoCYP94B1 from A. officinalis and its Arabidopsis ortholog AtCYP94B1 that are involved in apoplastic barrier formation, and are induced by 30 minutes of salt treatment in the roots. Heterologous expression of AoCYP94B1 in atcyp94b1 Arabidopsis mutant and wild-type rice conferred increased NaCl tolerance to seedlings by enhancing root suberin deposition. Histochemical staining and GC-MS/MS quantification of suberin precursors confirmed the role of CYP94B1 in suberin biosynthesis. Using chromatin immunoprecipitation, yeast one-hybrid and luciferase assays, we identified AtWRKY33 as the upstream regulator of AtCYP94B1 in Arabidopsis. In addition, atwrky33 mutants exhibited reduced suberin and salt sensitive phenotypes, which were rescued by expressing 35S::AtCYP94B1 in atwrky33 mutant. This further confirms that the regulation of AtCYP94B1 by AtWRKY33 is part of the salt tolerance mechanism, and our findings can help in generating salt tolerant crops.One sentence summaryAtWRKY33 transcription factor regulates AtCYP94B1 to increase plant salt tolerance by enhanced suberin deposition in the endodermal cells of Arabidopsis roots

Analysis of the aromatase cytochrome P450 gene in human breast cancers

1994 ◽  
Vol 13 (3) ◽  
pp. 331-337 ◽  
Author(s):  
P Sourdaine ◽  
M G Parker ◽  
J Telford ◽  
W R Miller
Keyword(s):  
Cytochrome P450 ◽  
Breast Cancers ◽  
Human Breast ◽  
Cytochrome P450 Gene ◽  
P450 Gene

Increase in pladienolide D production rate using a Streptomyces strain overexpressing a cytochrome P450 gene

2008 ◽  
Vol 105 (6) ◽  
pp. 649-654 ◽  
Author(s):  
Kazuhiro Machida ◽  
Yasuhide Aritoku ◽  
Takashi Nakashima ◽  
Akira Arisawa ◽  
Toshio Tsuchida
Keyword(s):  
Cytochrome P450 ◽  
Production Rate ◽  
Cytochrome P450 Gene ◽  
Streptomyces Strain ◽  
P450 Gene
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