scholarly journals The Tomato DELLA Protein PROCERA Promotes Abscisic Acid Responses in Guard Cells by Upregulating an Abscisic Acid Transporter

2020 ◽  
Vol 184 (1) ◽  
pp. 518-528
Author(s):  
Hagai Shohat ◽  
Natanella Illouz-Eliaz ◽  
Yuri Kanno ◽  
Mitsunori Seo ◽  
David Weiss
Keyword(s):  
Abscisic Acid ◽  
Guard Cells ◽  
Della Protein ◽  
Acid Transporter

scholarly journals The tomato DELLA protein PROCERA promotes ABA responses in guard cells by upregulating ABA transporter

2020 ◽  
Author(s):  
Hagai Shohat ◽  
Natanella Illouz-Eliaz ◽  
Yuri Kanno ◽  
Mitsunori Seo ◽  
David Weiss
Keyword(s):  
Gene Expression ◽  
Functional Analysis ◽  
Abscisic Acid ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Negative Interaction ◽  
Della Protein ◽  
Hormone Transport ◽  
Hormone Biosynthesis ◽  
Ga Response

AbstractPlants reduce transpiration to avoid drought stress by stomatal closure. While abscisic acid (ABA) has a central role in the regulation of stomatal closure under water-deficit conditions, we demonstrated in tomato that the gibberellin (GA) response inhibitor, the DELLA protein PROCERA (PRO), promotes ABA-induced stomatal closure and gene transcription in guard cells. To study how PRO affects stomatal closure, we performed RNAseq analysis of isolated guard cells and identified the ABA transporters ABA-IMPORTING TRANSPORTER1.1 (AIT1.1) and AIT1.2, also called in Arabidopsis NPF4.6, as upregulated by PRO. Tomato has four AIT1 genes, but only AIT1.1 and AIT1.2 were upregulated by PRO, and only AIT1.1 exhibited high expression in guard cells. Functional analysis of AIT1.1 in yeast confirmed its activity as an ABA transporter, possibly importer. CRISPR-Cas9-defrived ait1.1 mutant exhibited increased transpiration, larger stomatal aperture and reduced response to ABA. Moreover, ait1.1 suppressed the promoting effects of PRO on ABA-induced stomatal closure and gene expression in guard cells. The negative interaction between GA and ABA has been studied for many years in numerous plant species. These studies suggest that the crosstalk is mediated by changes in hormone biosynthesis and signaling. Our results suggest that it is also mediated by changes in hormone transport.One-sentence SummaryThe tomato DELLA protein PROCERA promoted abscisic acid-induced stomatal closure and gene expression by upregulating the expression of the ABA transporter ABA-IMPORTING TRANSPORTER 1 in guard cells.

scholarly journals Under salt stress guard cells rewire ion transport and abscisic acid (ABA) signaling

2021 ◽  
Author(s):  
Sohail M. Karimi ◽  
Matthias Freund ◽  
Brittney M. Wager ◽  
Michael Knoblauch ◽  
Jörg Fromm ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Salt Stress ◽  
Ion Transport ◽  
Guard Cells ◽  
Aba Signaling

scholarly journals Arabidopsis NPF4.6 and NPF5.1 Control Leaf Stomatal Aperture by Regulating Abscisic Acid Transport

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 885
Author(s):  
Takafumi Shimizu ◽  
Yuri Kanno ◽  
Hiromi Suzuki ◽  
Shunsuke Watanabe ◽  
Mitsunori Seo
Keyword(s):  
Abscisic Acid ◽  
Plant Hormone ◽  
Leaf Surface ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Cell Types ◽  
Acid Transport ◽  
Wild Type ◽  
Vascular Tissues ◽  
Abscisic Acid Transport

The plant hormone abscisic acid (ABA) is actively synthesized in vascular tissues and transported to guard cells to promote stomatal closure. Although several transmembrane ABA transporters have been identified, how the movement of ABA within plants is regulated is not fully understood. In this study, we determined that Arabidopsis NPF4.6, previously identified as an ABA transporter expressed in vascular tissues, is also present in guard cells and positively regulates stomatal closure in leaves. We also found that mutants defective in NPF5.1 had a higher leaf surface temperature compared to the wild type. Additionally, NPF5.1 mediated cellular ABA uptake when expressed in a heterologous yeast system. Promoter activities of NPF5.1 were detected in several leaf cell types. Taken together, these observations indicate that NPF5.1 negatively regulates stomatal closure by regulating the amount of ABA that can be transported from vascular tissues to guard cells.

scholarly journals Roles of intracellular hydrogen peroxide accumulation in abscisic acid signaling in Arabidopsis guard cells

2011 ◽  
Vol 168 (16) ◽  
pp. 1919-1926 ◽  
Author(s):  
Rayhanur Jannat ◽  
Misugi Uraji ◽  
Miho Morofuji ◽  
Mohammad Muzahidul Islam ◽  
Rachel E. Bloom ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Abscisic Acid ◽  
Guard Cells ◽  
Abscisic Acid Signaling ◽  
Hydrogen Peroxide Accumulation ◽  
Intracellular Hydrogen Peroxide

scholarly journals Effects of Cations and Abscisic Acid on Chlorophyll a Fluorescence in Guard Cells of Vicia faba

1982 ◽  
Vol 69 (5) ◽  
pp. 1140-1144 ◽  
Author(s):  
Teruo Ogawa ◽  
David Grantz ◽  
John Boyer ◽  
Govindjee
Keyword(s):  
Abscisic Acid ◽  
Chlorophyll A ◽  
Vicia Faba ◽  
Chlorophyll A Fluorescence ◽  
Guard Cells

Analysis of abscisic acid responsive proteins in Brassica napus guard cells by multiplexed isobaric tagging

2010 ◽  
Vol 73 (4) ◽  
pp. 790-805 ◽  
Author(s):  
Mengmeng Zhu ◽  
Brigitte Simons ◽  
Ning Zhu ◽  
David G. Oppenheimer ◽  
Sixue Chen
Keyword(s):  
Abscisic Acid ◽  
Brassica Napus ◽  
Guard Cells ◽  
Isobaric Tagging
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