scholarly journals The B3-Domain Transcription Factor VAL1 Regulates the Floral Transition by Repressing FLOWERING LOCUS T

2019 ◽  
Vol 181 (1) ◽  
pp. 236-248 ◽  
Author(s):  
Yanjun Jing ◽  
Qiang Guo ◽  
Rongcheng Lin
Keyword(s):  
Transcription Factor ◽  
Floral Transition ◽  
Flowering Locus T ◽  
Flowering Locus ◽  
B3 Domain

Structural insights into the multivalent binding of the Arabidopsis FLOWERING LOCUS T promoter by the CO-NF-Y master transcription factor complex

2021 ◽  
Author(s):  
Xinchen Lv ◽  
Xiaolin Zeng ◽  
Hongmiao Hu ◽  
Lixian Chen ◽  
Fan Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Molecular Model ◽  
Growth Cycle ◽  
Proximal Region ◽  
Floral Transition ◽  
Cis Elements ◽  
Flowering Locus T ◽  
Flowering Locus ◽  
Multivalent Binding ◽  
Transcription Factor Complex

Abstract Flowering plants sense various environmental and endogenous signals to trigger the floral transition and start the reproductive growth cycle. CONSTANS (CO) is a master transcription factor in the photoperiod floral pathway that integrates upstream signals and activates the florigen gene FLOWERING LOCUS T (FT). Here, we performed comprehensive structural and biochemical analyses to study the molecular mechanism underlying the regulation of FT by CO in Arabidopsis thaliana. We show that the four previously characterized cis-elements in the FT promoter proximal region, CORE1, CORE2, P1 and P2, are all direct CO binding sites. Structural analysis of CO in complex with NUCLEAR FACTOR-YB/YC (NF-YB/YC) and the CORE2 or CORE1 elements revealed the molecular basis for the specific recognition of the shared TGTG motifs. Biochemical analysis suggested that CO might form a homomultimeric assembly via its N-terminal B-Box domain and simultaneously occupy multiple cis-elements within the FT promoter. We suggest that this multivalent binding gives the CO-NF-Y complex high affinity and specificity for FT promoter binding. Overall, our data provide a detailed molecular model for the regulation of FT by the master transcription factor complex CO-NF-Y during the floral transition.

scholarly journals TEM1 combinatorially binds to FLOWERING LOCUS T and recruits a Polycomb factor to repress the floral transition in Arabidopsis

2021 ◽  
Vol 118 (35) ◽  
pp. e2103895118
Author(s):  
Hongmiao Hu ◽  
Shu Tian ◽  
Guohui Xie ◽  
Rui Liu ◽  
Nana Wang ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Molecular Basis ◽  
Floral Transition ◽  
Flowering Locus T ◽  
Dna Binding Domains ◽  
Adult Transition ◽  
Binding Domains ◽  
Flowering Locus ◽  
Specific Regulation

Arabidopsis TEMPRANILLO 1 (TEM1) is a transcriptional repressor that participates in multiple flowering pathways and negatively regulates the juvenile-to-adult transition and the flowering transition. To understand the molecular basis for the site-specific regulation of FLOWERING LOCUS T (FT) by TEM1, we determined the structures of the two plant-specific DNA-binding domains in TEM1, AP2 and B3, in complex with their target DNA sequences from the FT gene 5′-untranslated region (5′-UTR), revealing the molecular basis for TEM1 specificity for its DNA targets. In vitro binding assays revealed that the combination of the AP2 and B3 binding sites greatly enhanced the overall binding of TEM1 to the FT 5′-UTR, indicating TEM1 combinatorically recognizes the FT gene 5′-UTR. We further showed that TEM1 recruits the Polycomb repressive complex 2 (PRC2) to the FT 5′-UTR. The simultaneous binding of the TEM1 AP2 and B3 domains to FT is necessary for deposition of H3K27me3 at the FT 5′-UTR and for the flowering repressor function of TEM1. Overall, our data suggest that the combinatorial recognition of FT 5′-UTR by TEM1 ensures H3K27me3 deposition to precisely regulate the floral transition.

scholarly journals The sugar transporter SWEET10 acts downstream of FLOWERING LOCUS T during floral transition of Arabidopsis thaliana

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Fernando Andrés ◽  
Atsuko Kinoshita ◽  
Naveen Kalluri ◽  
Virginia Fernández ◽  
Vítor S. Falavigna ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Floral Transition ◽  
Sugar Transporter ◽  
Flowering Locus T ◽  
Flowering Locus

The orthologous Flowering Locus T (FT) and LEAFY (LFY) genes in the floral transition of Polianthes tuberosa

2020 ◽  
pp. 225-230
Author(s):  
J.C. Fragoso-Jimenez ◽  
D.E. Navarro-Lopez ◽  
R. Barba-Gonzalez ◽  
M.C. Castañeda-Saucedo ◽  
E. Tapia-Campos
Keyword(s):  
Floral Transition ◽  
Flowering Locus T ◽  
Flowering Locus

scholarly journals BRANCHED1 Interacts with FLOWERING LOCUS T to Repress the Floral Transition of the Axillary Meristems in Arabidopsis

2013 ◽  
Vol 25 (4) ◽  
pp. 1228-1242 ◽  
Author(s):  
Masaki Niwa ◽  
Yasufumi Daimon ◽  
Ken-ichi Kurotani ◽  
Asuka Higo ◽  
José L. Pruneda-Paz ◽  
...  
Keyword(s):  
Floral Transition ◽  
Flowering Locus T ◽  
Axillary Meristems ◽  
Flowering Locus

scholarly journals VDAC1 Negatively Regulates Floral Transition in Arabidopsis thaliana

2021 ◽  
Vol 22 (21) ◽  
pp. 11603
Author(s):  
Jingya Xu ◽  
Yuzhen Zhang ◽  
Hongjia Ren ◽  
Runyi Yu ◽  
Chen Yuan ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Growth Regulation ◽  
Floral Transition ◽  
Rna Seq ◽  
Wild Type ◽  
Anion Channels ◽  
Flowering Locus T ◽  
Plant Growth Regulation ◽  
Voltage Dependent ◽  
Flowering Locus

Voltage-dependent anion channels (VDACs) are the most important proteins in mitochondria. They localize to the outer mitochondrial membrane and contribute to the metabolite transport between the mitochondria and cytoplasm, which aids plant growth regulation. Here, we report that Arabidopsis thaliana VDAC1 is involved in the floral transition, with the loss of AtVDAC1 function, resulting in an early-flowering phenotype. AtVDAC1 is expressed ubiquitously in Arabidopsis. To identify the flowering pathway integrators that may be responsible for AtVDAC1′s function during the floral transition, an RNA-seq analysis was performed. In total, 106 differentially expressed genes (DEGs) were identified between wild-type and atvdac1-5 mutant seedlings. However, none were involved in flowering-related pathways. In contrast, AtVDAC1 physically associated with FLOWERING LOCUS T. Thus, in the floral transition, AtVDAC1 may function partly through the FLOWERING LOCUS T protein.

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