scholarly journals Dynamic Thylakoid Stacking Is Regulated by LHCII Phosphorylation but Not Its interaction with PSI

2019 ◽  
Vol 180 (4) ◽  
pp. 2152-2166 ◽  
Author(s):  
William H.J. Wood ◽  
Samuel F.H. Barnett ◽  
Sarah Flannery ◽  
C. Neil Hunter ◽  
Matthew P. Johnson
Keyword(s):  
Thylakoid Stacking

scholarly journals Publisher Correction: Dynamic thylakoid stacking and state transitions work synergistically to avoid acceptor-side limitation of photosystem I

Nature Plants ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 238-238
Author(s):  
Christopher Hepworth ◽  
William H. J. Wood ◽  
Tom Z. Emrich-Mills ◽  
Matthew S. Proctor ◽  
Stuart Casson ◽  
...  
Keyword(s):  
Photosystem I ◽  
State Transitions ◽  
Acceptor Side ◽  
Thylakoid Stacking

The effects of external NaCl on thylakoid stacking in lettuce plants

1993 ◽  
Vol 16 (2) ◽  
pp. 215-222 ◽  
Author(s):  
D. R. CARTER ◽  
J. M. CHEESEMAN
Keyword(s):  
Thylakoid Stacking

scholarly journals Acclimation of leaves to low light produces large grana: the origin of the predominant attractive force at work

2012 ◽  
Vol 367 (1608) ◽  
pp. 3494-3502 ◽  
Author(s):  
Husen Jia ◽  
John R. Liggins ◽  
Wah Soon Chow
Keyword(s):  
Thylakoid Membranes ◽  
Enthalpy Change ◽  
Attractive Force ◽  
Gibbs Free Energy Change ◽  
Photosynthetic Membrane ◽  
Low Light ◽  
Cyclic Photophosphorylation ◽  
Thylakoid Stacking ◽  
Endothermic Process ◽  
Fine Tune

Photosynthetic membrane sacs (thylakoids) of plants form granal stacks interconnected by non-stacked thylakoids, thereby being able to fine-tune (i) photosynthesis, (ii) photoprotection and (iii) acclimation to the environment. Growth in low light leads to the formation of large grana, which sometimes contain as many as 160 thylakoids. The net surface charge of thylakoid membranes is negative, even in low-light-grown plants; so an attractive force is required to overcome the electrostatic repulsion. The theoretical van der Waals attraction is, however, at least 20-fold too small to play the role. We determined the enthalpy change, in the spontaneous stacking of previously unstacked thylakoids in the dark on addition of Mg 2+ , to be zero or marginally positive (endothermic). The Gibbs free-energy change for the spontaneous process is necessarily negative, a requirement that can be met only by an increase in entropy for an endothermic process. We conclude that the dominant attractive force in thylakoid stacking is entropy-driven. Several mechanisms for increasing entropy upon stacking of thylakoid membranes in the dark, particularly in low-light plants, are discussed. In the light, which drives the chloroplast far away from equilibrium, granal stacking accelerates non-cyclic photophosphorylation, possibly enhancing the rate at which entropy is produced.

scholarly journals Investigations of the role of the main light-harvesting chlorophyll-protein complex in thylakoid membranes. Reconstitution of depleted membranes from intermittent-light-grown plants with the isolated complex.

1984 ◽  
Vol 98 (1) ◽  
pp. 163-172 ◽  
Author(s):  
D A Day ◽  
I J Ryrie ◽  
N Fuad
Keyword(s):  
Photosystem Ii ◽  
Light Harvesting ◽  
Gradient Centrifugation ◽  
Freeze Fracture ◽  
Direct Role ◽  
Lhc Ii ◽  
Intermittent Light ◽  
Light Harvesting Complex ◽  
Thylakoid Stacking

The functions of the light-harvesting complex of photosystem II (LHC-II) have been studied using thylakoids from intermittent-light-grown (IML) plants, which are deficient in this complex. These chloroplasts have no grana stacks and only limited lamellar appression in situ. In vitro the thylakoids showed limited but significant Mg2+-induced membrane appression and a clear segregation of membrane particles into such regions. This observation, together with the immunological detection of small quantities of LHC-II apoproteins, suggests that the molecular mechanism of appression may be similar to the more extensive thylakoid stacking seen in normal chloroplasts and involve LHC-II polypeptides directly. To study LHC-II function directly, a sonication-freeze-thaw procedure was developed for controlled insertion of purified LHC-II into IML membranes. Incorporation was demonstrated by density gradient centrifugation, antibody agglutination tests, and freeze-fracture electron microscopy. The reconstituted membranes, unlike the parent IML membranes, exhibited both extensive membrane appression and increased room temperature fluorescence in the presence of cations, and a decreased photosystem I activity at low light intensity. These membranes thus mimic normal chloroplasts in this regard, suggesting that the incorporated LHC-II interacts with photosystem II centers in IML membranes and exerts a direct role in the regulation of excitation energy distribution between the two photosystems.

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