scholarly journals Enzyme Fusion Removes Competition for Geranylgeranyl Diphosphate in Carotenogenesis

2018 ◽  
Vol 179 (3) ◽  
pp. 1013-1027 ◽  
Author(s):  
Maurizio Camagna ◽  
Alexander Grundmann ◽  
Cornelia Bär ◽  
Julian Koschmieder ◽  
Peter Beyer ◽  
...  
Keyword(s):  
Geranylgeranyl Diphosphate ◽  
Enzyme Fusion

scholarly journals Distribution of prenyltransferases in rat tissues. Evidence for a cytosolic all-trans-geranylgeranyl diphosphate synthase.

1993 ◽  
Vol 268 (2) ◽  
pp. 832-838
Author(s):  
J. Ericsson ◽  
M. Runquist ◽  
A. Thelin ◽  
M. Andersson ◽  
T. Chojnacki ◽  
...  
Keyword(s):  
Rat Tissues ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate

scholarly journals Lipophilic Bisphosphonates as Dual Farnesyl/Geranylgeranyl Diphosphate Synthase Inhibitors: An X-ray and NMR Investigation

2009 ◽  
Vol 131 (14) ◽  
pp. 5153-5162 ◽  
Author(s):  
Yonghui Zhang ◽  
Rong Cao ◽  
Fenglin Yin ◽  
Michael P. Hudock ◽  
Rey-Ting Guo ◽  
...  
Keyword(s):  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate ◽  
X Ray

scholarly journals The Branch Point Enzyme of the Mevalonate Pathway for Protein Prenylation Is Overexpressed in theob/ob Mouse and Induced by Adipogenesis

2000 ◽  
Vol 20 (6) ◽  
pp. 2158-2166 ◽  
Author(s):  
David Vicent ◽  
Eleftheria Maratos-Flier ◽  
C. Ronald Kahn
Keyword(s):  
Skeletal Muscle ◽  
Western Blot ◽  
Branch Point ◽  
Northern Blot ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Northern Blot Analysis ◽  
Geranylgeranyl Diphosphate ◽  
Protein Prenylation ◽  
Ggpp Synthase

ABSTRACT We have recently reported that skeletal muscle of theob/ob mouse, an animal model of genetic obesity with extreme insulin resistance, exhibits alterations in the expression of multiple genes. Analysis and cloning of a full-length cDNA of one of the overexpressed mRNAs revealed a 300-amino-acid protein that could be identified as the mouse geranylgeranyl diphosphate synthase (GGPP synthase) based on its homology to proteins cloned from yeast and fungus. GGPP synthase catalyzes the synthesis of all-trans-geranylgeranyl diphosphate (GGPP), an isoprenoid used for protein isoprenylation in animal cells, and is a branch point enzyme in the mevalonic acid pathway. Three mRNAs for GGPP synthase of 4.3, 3.2, and 1.7 kb were detected in Northern blot analysis. Western blot analysis of tissue homogenates using specific antipeptide antibodies revealed a single band of 34.8 kDa. Expression level of this protein in different tissues correlated with expression of the 4.3- and 3.2-kb mRNAs. GGPP synthase mRNA expression was increased 5- to 20-fold in skeletal muscle, liver, and fat of ob/obmice by Northern blot analysis. Western blot analysis also showed a twofold overexpression of the protein in muscle and fat but not in liver, where the dominant isoform is encoded by the 1.7-kb mRNA. Differentiation of 3T3-L1 fibroblasts into adipocytes induced GGPP synthase expression more than 20-fold. Using the immunoprecipitated protein, we found that mammalian GGPP synthase synthesizes not only GGPP but also its metabolic precursor farnesyl diphosphate. Thus, the expression of GGPP synthase is regulated in multiple tissues in obesity and is induced during adipocyte differentiation. Altered regulation in the synthesis of isoprenoids for protein prenylation in obesity might be a factor determining the ability of the cells to respond to hormonal stimulation requiring both Ras-related small GTPases and trimeric G protein-coupled receptors.

Studies on geranylgeranyl diphosphate synthase from rat liver: Specific inhibition by 3-azageranylgeranyl diphosphate

1992 ◽  
Vol 297 (2) ◽  
pp. 314-320 ◽  
Author(s):  
Hiroshi Sagami ◽  
Tatsumi Korenaga ◽  
Kyozo Ogura ◽  
Arthur Steiger ◽  
Hyung-Jung Pyun ◽  
...  
Keyword(s):  
Rat Liver ◽  
Specific Inhibition ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate

A diterpene synthase from the clary sage Salvia sclarea catalyzes the cyclization of geranylgeranyl diphosphate to (8R)-hydroxy-copalyl diphosphate

2013 ◽  
Vol 91 ◽  
pp. 93-99 ◽  
Author(s):  
Nils Günnewich ◽  
Yasuhiro Higashi ◽  
Xiaohong Feng ◽  
Kum-Boo Choi ◽  
Jürgen Schmidt ◽  
...  
Keyword(s):  
Geranylgeranyl Diphosphate ◽  
Salvia Sclarea ◽  
Diterpene Synthase
Sign in / Sign up

Export Citation Format

Share Document