scholarly journals MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements

2018 ◽  
Vol 178 (4) ◽  
pp. 1436-1447 ◽  
Author(s):  
Huawen Lin ◽  
Paul F. Cliften ◽  
Susan K. Dutcher
Keyword(s):  
Detection Method ◽  
Dna Rearrangements ◽  
Highly Efficient ◽  
Efficient Detection

Electrochemical sensing of H2O2 released from living cells based on AuPd alloy-modified PDA nanotubes

2019 ◽  
Vol 11 (12) ◽  
pp. 1651-1656 ◽  
Author(s):  
Guangli He ◽  
Fengli Gao ◽  
Wei Li ◽  
Pengwei Li ◽  
Xiaofan Zhang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Detection Method ◽  
Living Cells ◽  
Electrochemical Sensing ◽  
Highly Efficient ◽  
Efficient Detection ◽  
Significant Attention

The highly efficient detection method for hydrogen peroxide (H2O2) has been attracting significant attention.

scholarly journals Recombinase-Aided Amplification Coupled with Lateral Flow Dipstick for Efficient and Accurate Detection of Porcine Parvovirus

Life ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 762
Author(s):  
Yihong He ◽  
Wenxian Chen ◽  
Jindai Fan ◽  
Shuangqi Fan ◽  
Hongxing Ding ◽  
...  
Keyword(s):  
Detection Method ◽  
Cross Reactivity ◽  
Lateral Flow ◽  
Porcine Parvovirus ◽  
Swine Industry ◽  
Resource Limited ◽  
Efficient Detection ◽  
Lateral Flow Dipstick ◽  
Polymerase Chain ◽  
Embryonic Death

Porcine parvovirus (PPV) infection is the primary cause of SMEDI (stillbirth; mummification; embryonic death; infertility) syndrome, which is a global burden for the swine industry. Thus, it is crucial to establish a rapid and efficient detection method against PPV infection. In the present work, we developed a recombinase-aided amplification (RAA) assay, coupled with a lateral flow dipstick (LFD), to achieve an amplification of PPV DNA at 37 °C within 15 min. The detection limits of PPV RAA-LFD assay were 102 copies/μL recombinant plasmid pMD19-T-VP1, 6.38 × 10−7 ng/μL PPV DNA, and 10−1 TCID50/mL virus, respectively. This method was highly specific for PPV detection with no cross-reactivity for other swine pathogens. In contrast to polymerase chain reaction (PCR), the PPV RAA-LFD assay is more sensitive and cost-saving. Hence, the established PPV RAA-LFD assay provided an alternative for PPV detection, especially in resource-limited regions.

scholarly journals Immunomagnetic bead-based biotin-streptavidin system for highly efficient detection of aflatoxin B1 in agricultural products

RSC Advances ◽  
2018 ◽  
Vol 8 (46) ◽  
pp. 26029-26035 ◽  
Author(s):  
Ming Li ◽  
Yuanyuan Zhang ◽  
Rujin Zhao ◽  
Zhenjiang Liu ◽  
Xia Hong ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Agricultural Products ◽  
Immunomagnetic Beads ◽  
Immunomagnetic Bead ◽  
Highly Efficient ◽  
Efficient Detection

A strategy of immunomagnetic beads coupled with biotin-streptavidin system (BAS-IMB) for detecting AFB1 was suggested and applied in agricultural products.

Highly Efficient Detection of Homologues and Isomers by the Dynamic Swelling Reflection Spectrum

2020 ◽  
Vol 12 (40) ◽  
pp. 45174-45183
Author(s):  
Yang Hu ◽  
Yuqi Zhang ◽  
Tong Chen ◽  
Dongpeng Yang ◽  
Dekun Ma ◽  
...  
Keyword(s):  
Reflection Spectrum ◽  
Highly Efficient ◽  
Efficient Detection ◽  
Dynamic Swelling
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