scholarly journals Spatial Control of Gene Expression by miR319-Regulated TCP Transcription Factors in Leaf Development

2017 ◽  
Vol 176 (2) ◽  
pp. 1694-1708 ◽  
Author(s):  
Edgardo G. Bresso ◽  
Uciel Chorostecki ◽  
Ramiro E. Rodriguez ◽  
Javier F. Palatnik ◽  
Carla Schommer
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Leaf Development ◽  
Control Of Gene Expression ◽  
Spatial Control

Principles of Molecular Biology

2013 ◽  
pp. 76-87
Author(s):  
Steven E. Hyman ◽  
Eric J. Nestler
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Molecular Biology ◽  
Cell Surface ◽  
Environmental Context ◽  
Molecular Processes ◽  
Control Of Gene Expression ◽  
Extracellular Signals ◽  
Gene Environment

This chapter provides an overview of the fundamental molecular processes by which information is encoded in the genome and how this information is expressed within an environmental context. We describe what genes are, how they function, and how their expression into RNA and protein is regulated by signals from outside the cell. Particular attention is given to a series of stimulus-regulated transcription factors, which play important roles in transducing information from the cell surface to the nucleus. Work in this area has shown that the control of gene expression by extracellular signals is a critical arena for gene–environment interactions that are highly relevant to psychiatry.

The control of gene expression and cell proliferation by the epithelial apical junctional complex

2012 ◽  
Vol 53 ◽  
pp. 83-93 ◽  
Author(s):  
Domenica Spadaro ◽  
Rocio Tapia ◽  
Pamela Pulimeno ◽  
Sandra Citi
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Transcription Factors ◽  
Protein Complexes ◽  
Regulation Of Gene Expression ◽  
Small Gtpases ◽  
Junctional Complex ◽  
Nucleotide Exchange ◽  
Control Of Gene Expression ◽  
Apical Junctional Complex

The AJC (apical junctional complex) of vertebrate epithelial cells orchestrates cell–cell adhesion and tissue barrier function. In addition, it plays a pivotal role in signalling. Several protein components of the AJC, e.g. the cytoplasmic proteins β-catenin, p120-catenin and ZO (Zonula Occludens)-2, can shuttle to the nucleus, where they interact with transcription factors to regulate gene expression and cell proliferation. Other junctional proteins, e.g. angiomotin, α-catenin and cingulin, are believed to act by sequestering either transcription factors, such as YAP (Yes-associated protein), or regulators of small GTPases, such as GEF (guanine-nucleotide-exchange factor)-H1, at junctions. The signalling activities of AJC proteins are triggered by different extracellular and intracellular cues, including cell density, and physiological or pathological activation of developmentally regulated pathways, such as the Wnt pathway. The interplay between junctional protein complexes, the actin cytoskeleton and signalling pathways is of crucial importance in the regulation of gene expression and cell proliferation.

Intranuclear trafficking of transcription factors: Requirements for vitamin D-mediated biological control of gene expression

2002 ◽  
Vol 88 (2) ◽  
pp. 340-355 ◽  
Author(s):  
Gary S. Stein ◽  
Jane B. Lian ◽  
Janet L. Stein ◽  
André J. van Wijnen ◽  
Martin Montecino ◽  
...  
Keyword(s):  
Gene Expression ◽  
Biological Control ◽  
Vitamin D ◽  
Transcription Factors ◽  
Control Of Gene Expression

scholarly journals The histone H3K4 demethylase JARID1A directly interacts with haematopoietic transcription factor GATA1 in erythroid cells through its second PHD domain

2020 ◽  
Vol 7 (1) ◽  
pp. 191048 ◽  
Author(s):  
Dimple Karia ◽  
Robert C. G. Gilbert ◽  
Antonio J. Biasutto ◽  
Catherine Porcher ◽  
Erika J. Mancini
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Blood Cells ◽  
Physical Contact ◽  
Histone Demethylases ◽  
Control Of Gene Expression ◽  
Histone H3k4 ◽  
Lineage Specific Genes ◽  
Phd Domain

Chromatin remodelling and transcription factors play important roles in lineage commitment and development through control of gene expression. Activation of selected lineage-specific genes and repression of alternative lineage-affiliated genes result in tightly regulated cell differentiation transcriptional programmes. However, the complex functional and physical interplay between transcription factors and chromatin-modifying enzymes remains elusive. Recent evidence has implicated histone demethylases in normal haematopoietic differentiation as well as in malignant haematopoiesis. Here, we report an interaction between H3K4 demethylase JARID1A and the haematopoietic-specific master transcription proteins SCL and GATA1 in red blood cells. Specifically, we observe a direct physical contact between GATA1 and the second PHD domain of JARID1A. This interaction has potential implications for normal and malignant haematopoiesis.

scholarly journals Spatial control of gene expression within a scaffold by localized inducer release

Biomaterials ◽  
2011 ◽  
Vol 32 (11) ◽  
pp. 3062-3071 ◽  
Author(s):  
Priya R. Baraniak ◽  
Devin M. Nelson ◽  
Cory E. Leeson ◽  
Anand K. Katakam ◽  
Jennifer L. Friz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Control Of Gene Expression ◽  
Spatial Control

scholarly journals The Mechanisms of Generation, Recognition, and Erasure of DNA 5-Methylcytosine and Thymine Oxidations

2015 ◽  
Vol 290 (34) ◽  
pp. 20723-20733 ◽  
Author(s):  
Hideharu Hashimoto ◽  
Xing Zhang ◽  
Paula M. Vertino ◽  
Xiaodong Cheng
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Cytosine Methylation ◽  
Epigenetic Modifications ◽  
Dna Bases ◽  
Oxidation States ◽  
Control Of Gene Expression ◽  
Methylation Of Dna ◽  
Recent Developments ◽  
Base Excision

One of the most fundamental questions in the control of gene expression in mammals is how the patterns of epigenetic modifications of DNA are generated, recognized, and erased. This includes covalent cytosine methylation of DNA and its associated oxidation states. An array of AdoMet-dependent methyltransferases, Fe(II)- and α-ketoglutarate-dependent dioxygenases, base excision glycosylases, and sequence-specific transcription factors is responsible for changing, maintaining, and interpreting the modification status of specific regions of chromatin. This review focuses on recent developments in characterizing the functional and structural links between the modification status of two DNA bases 5-methylcytosine and thymine (5-methyluracil).

scholarly journals Translational regulation of protrusion-localized RNAs involves silencing and clustering after transport

2019 ◽  
Author(s):  
Konstadinos Moissoglu ◽  
Kyota Yasuda ◽  
Tianhong Wang ◽  
George Chrisafis ◽  
Stavroula Mili
Keyword(s):  
Gene Expression ◽  
Translational Regulation ◽  
Cellular Responses ◽  
Distinct Mode ◽  
Control Of Gene Expression ◽  
Spatial Control ◽  
Polarized Cells ◽  
Regulate Protein ◽  
In Transit ◽  
Human And Mouse

ABSTRACTLocalization of RNAs to various subcellular destinations is a widely used mechanism that regulates a large proportion of transcripts in polarized cells. In many cases, such localized transcripts mediate spatial control of gene expression by being translationally silent while in transit and locally activated at their destination. Here, we investigate the translation of RNAs localized at dynamic cellular protrusions of human and mouse, migrating, mesenchymal cells. In contrast to the model described above, we find that protrusion-localized RNAs are not locally activated solely at protrusions, but can be translated with similar efficiency in both internal and peripheral locations. Interestingly, protrusion-localized RNAs are translated at extending protrusions, they become translationally silenced in retracting protrusions and this silencing is accompanied by coalescence of single RNAs into larger heterogeneous RNA clusters. This work describes a distinct mode of translational regulation of localized RNAs, which we propose is used to regulate protein activities during dynamic cellular responses.

scholarly journals CreLite: An Optogenetically Controlled Cre/loxP System Using Red Light

2019 ◽  
Author(s):  
Shuo-Ting Yen ◽  
Kenneth A. Trimmer ◽  
Nader Aboul ◽  
Rachel D. Mullen ◽  
James C. Culver ◽  
...  
Keyword(s):  
Gene Expression ◽  
Light Exposure ◽  
Red Light ◽  
Regulatory Elements ◽  
Cell Labeling ◽  
P System ◽  
Zebrafish Embryos ◽  
Control Of Gene Expression ◽  
Spatial Control ◽  
Temporal And Spatial

ABSTRACTPrecise manipulation of gene expression with temporal and spatial control is essential for functional studies and the determination of cell lineage relationships in complex biological systems. The Cre-loxP system is commonly used for gene manipulation at desired times and places. However, specificity is dependent on the availability of tissue- or cell-specific regulatory elements used in combination with Cre or CreER (tamoxifen-inducible). Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos. Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6. In addition, PhyB-PIF6 binding requires phycocyanobilin (PCB), providing an additional layer of control. Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity. Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter (ubi:zebrabow) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium. We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development. CreLite provides a novel optogenetic tool for precise temporal and spatial control of gene expression in zebrafish embryos that may also be useful in cell culture, ex vivo organ culture, and other animal models for developmental biology studies.

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