scholarly journals Two Chloroplast Proteins Suppress Drought Resistance by Affecting ROS Production in Guard Cells

2016 ◽  
Vol 172 (4) ◽  
pp. 2491-2503 ◽  
Author(s):  
Zhen Wang ◽  
Fuxing Wang ◽  
Yechun Hong ◽  
Jirong Huang ◽  
Huazhong Shi ◽  
...  
Keyword(s):  
Drought Resistance ◽  
Guard Cells ◽  
Ros Production ◽  
Chloroplast Proteins

scholarly journals Two Chloroplast Proteins Negatively Regulate Plant Drought Resistance Through Separate Pathways

2019 ◽  
Vol 182 (2) ◽  
pp. 1007-1021 ◽  
Author(s):  
Yechun Hong ◽  
Zhen Wang ◽  
Xue Liu ◽  
Juanjuan Yao ◽  
Xiangfeng Kong ◽  
...  
Keyword(s):  
Drought Resistance ◽  
Chloroplast Proteins

scholarly journals Are Grapevine Stomata Involved in the Elicitor-Induced Protection Against Downy Mildew?

2009 ◽  
Vol 22 (8) ◽  
pp. 977-986 ◽  
Author(s):  
Mathilde Allègre ◽  
Marie-Claire Héloir ◽  
Sophie Trouvelot ◽  
Xavier Daire ◽  
Alain Pugin ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Plasmopara Viticola ◽  
Ros Production ◽  
Plant Leaves ◽  
Gas Exchanges ◽  
Defense Reactions ◽  
Signaling Events ◽  
Stomatal Movements

Stomata, natural pores bordered by guard cells, regulate transpiration and gas exchanges between plant leaves and the atmosphere. These natural openings also constitute a way of penetration for microorganisms. In plants, the perception of potentially pathogenic microorganisms or elicitors of defense reactions induces a cascade of events, including H2O2 production, that allows the activation of defense genes, leading to defense reactions. Similar signaling events occur in guard cells in response to the perception of abscisic acid (ABA), leading to stomatal closure. Moreover, few elicitors were reported to induce stomatal closure in Arabidopsis and Vicia faba leaves. Because responses to ABA and elicitors share common signaling events, it led us to question whether stomatal movements and H2O2 production in guard cells could play a key role in elicitor-induced protection against pathogens that use stomata for infection. This study was performed using the grapevine–Plasmopara viticola pathosystem. Using epidermal peels, we showed that, as for ABA, the elicitor-induced stomatal closure is mediated by reactive oxygen species (ROS) production in guard cells. In plants, we observed that the protection against downy mildew induced by some elicitors is probably not due only to effects on stomatal movements or to a guard-cell-specific activation of ROS production.

scholarly journals The Myrosinases TGG1 and TGG2 Function Redundantly in Reactive Carbonyl Species Signaling in Arabidopsis Guard Cells

2020 ◽  
Vol 61 (5) ◽  
pp. 967-977 ◽  
Author(s):  
Mohammad Saidur Rhaman ◽  
Toshiyuki Nakamura ◽  
Yoshimasa Nakamura ◽  
Shintaro Munemasa ◽  
Yoshiyuki Murata
Keyword(s):  
Stomatal Closure ◽  
Guard Cells ◽  
Signal Pathway ◽  
Ethylene Glycol Tetraacetic Acid ◽  
Free Calcium ◽  
Ros Production ◽  
Reactive Carbonyl Species ◽  
Aldehydes And Ketones ◽  
Carbonyl Species ◽  
Reactive Carbonyl

Abstract Myrosinase (β-thioglucoside glucohydrolase, enzyme nomenclature, EC 3.2.1.147, TGG) is a highly abundant protein in Arabidopsis guard cells, of which TGG1 and TGG2 function redundantly in abscisic acid (ABA)- and methyl jasmonate-induced stomatal closure. Reactive carbonyl species (RCS) are α,β-unsaturated aldehydes and ketones, which function downstream of reactive oxygen species (ROS) production in the ABA signalling pathway in guard cells. Among the RCS, acrolein is the most highly reactive, which is significantly produced in ABA-treated guard cells. To clarify the ABA signal pathway downstream of ROS production, we investigated the responses of tgg mutants (tgg1-3, tgg2-1 and tgg1-3 tgg2-1) to acrolein. Acrolein induced stomatal closure and triggered cytosolic alkalization in wild type (WT), tgg1-3 single mutants and in tgg2-1 single mutants, but not in tgg1-3 tgg2-1 double mutants. Exogenous Ca2+ induced stomatal closure and cytosolic alkalization not only in WT but also in all of the mutants. Acrolein- and Ca2+-induced stomatal closures were inhibited by an intracellular acidifying agent, butyrate, a Ca2+ chelator, ethylene glycol tetraacetic acid (EGTA) and a Ca2+ channel blocker, LaCl3. Acrolein induced cytosolic free calcium concentration ([Ca2+]cyt) elevation in guard cells of WT plants but not in the tgg1-3 tgg2-1 double mutants. Exogenous Ca2+ elicited [Ca2+]cyt elevation in guard cells of WT and tgg1-3 tgg2-1. Our results suggest that TGG1 and TGG2 function redundantly, not between ROS production and RCS production, but downstream of RCS production in the ABA signal pathway in Arabidopsis guard cells.

scholarly journals Ethylene Inhibits Methyl Jasmonate-Induced Stomatal Closure by Modulating Guard Cell Slow-Type Anion Channel Activity via the OPEN STOMATA 1/SnRK2.6 Kinase-Independent Pathway in Arabidopsis

2019 ◽  
Vol 60 (10) ◽  
pp. 2263-2271 ◽  
Author(s):  
Shintaro Munemasa ◽  
Yukari Hirao ◽  
Kasumi Tanami ◽  
Yoshiharu Mimata ◽  
Yoshimasa Nakamura ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Guard Cell ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Defense Responses ◽  
Ros Production ◽  
Ethylene Signaling ◽  
Aba Signaling ◽  
Anion Channels ◽  
Signal Crosstalk

Abstract Signal crosstalk between jasmonate and ethylene is crucial for a proper maintenance of defense responses and development. Although previous studies reported that both jasmonate and ethylene also function as modulators of stomatal movements, the signal crosstalk mechanism in stomatal guard cells remains unclear. Here, we show that the ethylene signaling inhibits jasmonate signaling as well as abscisic acid (ABA) signaling in guard cells of Arabidopsis thaliana and reveal the signaling crosstalk mechanism. Both an ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and an ethylene-releasing compound ethephon induced transient stomatal closure, and also inhibited methyl jasmonate (MeJA)-induced stomatal closure as well as ABA-induced stomatal closure. The ethylene inhibition of MeJA-induced stomatal closure was abolished in the ethylene-insensitive mutant etr1–1, whereas MeJA-induced stomatal closure was impaired in the ethylene-overproducing mutant eto1–1. Pretreatment with ACC inhibited MeJA-induced reactive oxygen species (ROS) production as well as ABA-induced ROS production in guard cells but did not suppress ABA activation of OPEN STOMATA 1 (OST1) kinase in guard cell-enriched epidermal peels. The whole-cell patch-clamp analysis revealed that ACC attenuated MeJA and ABA activation of S-type anion channels in guard cell protoplasts. However, MeJA and ABA inhibitions of Kin channels were not affected by ACC pretreatment. These results suggest that ethylene signaling inhibits MeJA signaling and ABA signaling by targeting S-type anion channels and ROS but not OST1 kinase and K+ channels in Arabidopsis guard cells.

A Scanning Electron Microscope Study of Isolated Guard Cells

1973 ◽  
Vol 31 ◽  
pp. 454-455 ◽  
Author(s):  
P. Dayanandan ◽  
P. B. Kaufman
Keyword(s):  
Electron Microscope ◽  
Electron Microscope Study ◽  
Three Dimensional ◽  
Guard Cells ◽  
Scanning Electron Microscope Study ◽  
Psilotum Nudum ◽  
Subsidiary Cells ◽  
Welwitschia Mirabilis ◽  
Cycas Revoluta ◽  
Scanning Electron

A three dimensional appreciation of the guard cell morphology coupled with ultrastjuctural studies should lead to a better understanding of their still obscure dynamics of movement. We have found the SEM of great value not only in studies of the surface details of stomata but also in resolving the structures and relationships that exist between the guard and subsidiary cells. We now report the isolation and SEM studies of guard cells from nine genera of plants.Guard cells were isolated from the following plants: Psilotum nudum, four species of Equisetum, Cycas revoluta, Ceratozamia sp., Pinus sylvestris, Ephedra cochuma, Welwitschia mirabilis, Euphorbia tirucalli and Allium cepa.

1703-P: Altered UCP1 Expression in ADSC-Derived Beige Adipocytes Determines Mitochondrial ROS Production in T2DM Patients

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 1703-P
Author(s):  
SVETLANA MICHURINA ◽  
IURII STAFEEV ◽  
IGOR SKLYANIK ◽  
EKATERINA SHESTAKOVA ◽  
ANATOLIY YURASOV ◽  
...  
Keyword(s):  
Ros Production ◽  
Mitochondrial Ros ◽  
Beige Adipocytes ◽  
Ucp1 Expression
Sign in / Sign up

Export Citation Format

Share Document