scholarly journals Chlamydomonas reinhardtii PsbS Protein Is Functional and Accumulates Rapidly and Transiently under High Light

2016 ◽  
Vol 171 (4) ◽  
pp. 2717-2730 ◽  
Author(s):  
Tania Tibiletti ◽  
Pascaline Auroy ◽  
Gilles Peltier ◽  
Stefano Caffarri
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
High Light ◽  
Psbs Protein

scholarly journals Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii

2016 ◽  
Vol 291 (33) ◽  
pp. 17478-17487 ◽  
Author(s):  
Viviana Correa-Galvis ◽  
Petra Redekop ◽  
Katharine Guan ◽  
Annika Griess ◽  
Thuy B. Truong ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Chlamydomonas Reinhardtii ◽  
Conformational Changes ◽  
Light Exposure ◽  
Complex Stress ◽  
High Light ◽  
Photochemical Quenching ◽  
Light Illumination ◽  
Non Photochemical Quenching ◽  
Psbs Protein

Non-photochemical quenching of excess excitation energy is an important photoprotective mechanism in photosynthetic organisms. In Arabidopsis thaliana, a high quenching capacity is constitutively present and depends on the PsbS protein. In the green alga Chlamydomonas reinhardtii, non-photochemical quenching becomes activated upon high light acclimation and requires the accumulation of light harvesting complex stress-related (LHCSR) proteins. Expression of the PsbS protein in C. reinhardtii has not been reported yet. Here, we show that PsbS is a light-induced protein in C. reinhardtii, whose accumulation under high light is further controlled by CO2 availability. PsbS accumulated after several hours of high light illumination at low CO2. At high CO2, however, PsbS was only transiently expressed under high light and was degraded after 1 h of high light exposure. PsbS accumulation correlated with an enhanced non-photochemical quenching capacity in high light-acclimated cells grown at low CO2. However, PsbS could not compensate for the function of LHCSR in an LHCSR-deficient mutant. Knockdown of PsbS accumulation led to reduction of both non-photochemical quenching capacity and LHCSR3 accumulation. Our data suggest that PsbS is essential for the activation of non-photochemical quenching in C. reinhardtii, possibly by promoting conformational changes required for activation of LHCSR3-dependent quenching in the antenna of photosystem II.

scholarly journals Photoinhibition and continuous growth of the wild-type and a high-light tolerant strain of Chlamydomonas reinhardtii

2019 ◽  
Vol 57 (2) ◽  
pp. 617-626 ◽  
Author(s):  
O. VIRTANEN ◽  
D. VALEV ◽  
O. KRUSE ◽  
L. WOBBE ◽  
E. TYYSTJARVI
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
High Light ◽  
Wild Type ◽  
Continuous Growth ◽  
Tolerant Strain

scholarly journals The role of LHCBM1 in non-photochemical quenching in Chlamydomonas reinhardtii

2022 ◽  
Author(s):  
Xin Liu ◽  
Wojciech J Nawrocki ◽  
Roberta Croce
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Protein Complexes ◽  
Ph Sensor ◽  
High Light ◽  
Photochemical Quenching ◽  
Knock Out ◽  
Photosynthetic Organisms ◽  
Pigment Protein Complexes ◽  
Non Photochemical Quenching

Non-photochemical quenching (NPQ) is the process that protects photosynthetic organisms from photodamage by dissipating the energy absorbed in excess as heat. In the model green alga Chlamydomonas reinhardtii, NPQ was abolished in the knock-out mutants of the pigment-protein complexes LHCSR3 and LHCBM1. However, while LHCSR3 was shown to be a pH sensor and switching to a quenched conformation at low pH, the role of LHCBM1 in NPQ has not been elucidated yet. In this work, we combine biochemical and physiological measurements to study short-term high light acclimation of npq5, the mutant lacking LHCBM1. We show that while in low light in the absence of this complex, the antenna size of PSII is smaller than in its presence, this effect is marginal in high light, implying that a reduction of the antenna is not responsible for the low NPQ. We also show that the mutant expresses LHCSR3 at the WT level in high light, indicating that the absence of this complex is also not the reason. Finally, NPQ remains low in the mutant even when the pH is artificially lowered to values that can switch LHCSR3 to the quenched conformation. It is concluded that both LHCSR3 and LHCBM1 need to be present for the induction of NPQ and that LHCBM1 is the interacting partner of LHCSR3. This interaction can either enhance the quenching capacity of LHCSR3 or connect this complex with the PSII supercomplex.

Expression of the high light-inducible Dunaliella LIP promoter in Chlamydomonas reinhardtii

Planta ◽  
2013 ◽  
Vol 238 (6) ◽  
pp. 1147-1156 ◽  
Author(s):  
Seunghye Park ◽  
Yew Lee ◽  
Jae-Hyeok Lee ◽  
EonSeon Jin
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
High Light

Chlamydomonas reinhardtii responding to high light: a role for 2-propenal (acrolein)

2017 ◽  
Vol 161 (1) ◽  
pp. 75-87 ◽  
Author(s):  
Thomas Roach ◽  
Theresa Baur ◽  
Wolfgang Stöggl ◽  
Anja Krieger-Liszkay
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
High Light

Multiple stressor effects of high light irradiance and photosynthetic herbicides on growth and survival of the green alga Chlamydomonas reinhardtii

2010 ◽  
Vol 29 (10) ◽  
pp. 2211-2219 ◽  
Author(s):  
Beat B. Fischer ◽  
Karin Rüfenacht ◽  
Kerstin Dannenhauer ◽  
Manuela Wiesendanger ◽  
Rik I.L. Eggen
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
High Light ◽  
Growth And Survival ◽  
Light Irradiance ◽  
Multiple Stressor
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