scholarly journals Lack of FTSH4 Protease Affects Protein Carbonylation, Mitochondrial Morphology, and Phospholipid Content in Mitochondria of Arabidopsis: New Insights into a Complex Interplay

2016 ◽  
Vol 171 (4) ◽  
pp. 2516-2535 ◽  
Author(s):  
Elwira Smakowska ◽  
Renata Skibior-Blaszczyk ◽  
Malgorzata Czarna ◽  
Marta Kolodziejczak ◽  
Malgorzata Kwasniak-Owczarek ◽  
...  
Keyword(s):  
Protein Carbonylation ◽  
Phospholipid Content ◽  
Mitochondrial Morphology ◽  
Complex Interplay

Abstract 344: Basis for Bicarbonate Damage in Myocardial Ischemia/Reperfusion Injury

2014 ◽  
Vol 115 (suppl_1) ◽  
Author(s):  
Bruno B Queliconi ◽  
Fernanda M Cunha ◽  
Roberta A Gottlieb ◽  
Alicia J Kowaltowski
Keyword(s):  
Cell Death ◽  
Ischemia Reperfusion ◽  
Protein Aggregates ◽  
Protein Carbonylation ◽  
Redox Activity ◽  
Mitochondrial Morphology ◽  
Ros Production ◽  
High Co2 ◽  
Rat Hearts ◽  
Perfused Rat Hearts

In the clinical setting, bicarbonate is often used to correct acidosis arising from accumulated CO2/HCO3- during ischemia. We observed that HL-1 cardiomyocytes exposed to increased [CO2/HCO3-] had more cell death after hypoxia/reoxygenation (H/R) and Langendorff-perfused rat hearts had larger infarcts after ischemia/reperfusion (I/R). In addition to buffering pH, the CO2/HCO3- pair possess an underappreciated redox activity that may contribute to injury. In order to study the effects of high CO2/HCO3- during ischemic injury independent of pH, we clamped pH using HEPES and used the mouse cardiomyocyte HL-1 cell line and isolated perfused rat hearts. HL-1 cells exposed to 10% CO2/HCO3- had no damage under basal conditions but developed exaggerated protein carbonylation and cell death after H/R. In Langendorff-perfused rat hearts, 10% CO2 was well tolerated during baseline conditions but resulted in increased protein carbonylation, cell death and larger infarcts after I/R. We hypothesized that the increased oxidative damage to proteins could be due to mitochondrial dysfunction with greater ROS production, diminished proteasomal degradation of oxidized proteins, or impaired autophagic clearance of damaged mitochondria and oxidized protein aggregates. There was no differential effect of CO2 on mitochondrial morphology or proteasomal activity in HL-1 cells. In mitochondria isolated from perfused hearts subjected to I/R under low and high CO2 conditions, there was no difference in ROS production or oxidized protein content, suggesting mitochondrial damage was not affected by CO2 level. Examination of autophagy in HL-1 cells exposed to high CO2 during H/R revealed higher LC3-II and lower p62 content. In hearts, changes in LC3-II were inconsistent; however, we detected less p62 protein, less mitochondria-associated Beclin1, and significantly more LC3 mRNA in hearts exposed to 10% CO2 during I/R. Taken together, these findings suggest that 10% CO2 affects autophagy, which could explain the accumulation of oxidatively damaged proteins. These findings point to a protective role for autophagic clearance of oxidized protein aggregates during I/R injury that may be adversely impacted by bicarbonate therapy.

‘Nothing is simply one thing’: Woolf, Deleuze and Difference

2013 ◽  
Vol 7 (4) ◽  
pp. 456-474
Author(s):  
Beatrice Monaco
Keyword(s):  
Virginia Woolf ◽  
Prose Poetry ◽  
Close Reading ◽  
Similar Model ◽  
Complex Interplay ◽  
To The Lighthouse

This paper explores some key texts of Virginia Woolf in the context of Deleuzian concepts. Using a close reading style, it shows how the prose poetry in Mrs Dalloway engages a complex interplay of repetition and difference, resulting in a remarkably similar model of the three syntheses of time as Deleuze understands them. It subsequently explores Woolf's technical processes in a key passage from To the Lighthouse, showing how the prose-poetic technique systematically undoes the structures of logical fact and rationality inscribed in both language and everyday speech to an extremely precise level.

Introduction: Exploring Russian nationalisms

2018 ◽  
Author(s):  
Pål Kolstø ◽  
Helge Blakkisrud
Keyword(s):  
The State ◽  
Complex Interplay ◽  
Recent Developments ◽  
Part Structure

Russian societal nationalism comes in various guises, both ethnic and imperialist. Also Putin’s rhetoric is marked by the tensions between ethnic and state-focused, imperialist thinking. Noting the complex interplay of state nationalism and societal nationalism, this introductory chapter examines the mental framework within which Russian politicians were acting prior to the decision to annex Crimea. The chapter develops a typology of Russian nationalisms, surveys recent developments, and presents the three-part structure of this book: official nationalism, radical and other societal nationalisms, and identities/otherings. It concludes that after the annexation of Crimea, when the state took over the agenda of both ethnic and imperialist nationalists in Russia, societal nationalism finds itself at low ebb.

Cellulose Separators with Integrated Carbon Nanotube Interlayers for Lithium-Sulfur Batteries: An Investigation into the Complex Interplay Between Cell Components

2019 ◽  
Author(s):  
Yu-Chuan Chien ◽  
Ruijun Pan ◽  
Ming-Tao Lee ◽  
Leif Nyholm ◽  
Daniel Brandell ◽  
...  
Keyword(s):  
Coulombic Efficiency ◽  
Solid Electrolyte Interphase ◽  
Holistic Approach ◽  
Cycle Life ◽  
Complex Interplay ◽  
Positive Electrodes ◽  
Lithium Sulfur Batteries ◽  
Cell Components ◽  
Redox Shuttle ◽  
Lithium Sulfur

This work aims to address two major roadblocks in the development of lithium-sulfur (Li-S) batteries: the inefficient deposition of Li on the metallic Li electrode and the parasitic "polysulfide redox shuttle". These roadblocks are here approached, respectively, by the combination of a cellulose separator with a cathode-facing conductive porous carbon interlayer, based on their previously reported individual benefits. The cellulose separator increases cycle life by 33%, and the interlayer by a further 25%, in test cells with positive electrodes with practically relevant specifications and a relatively low electrolyte/sulfur (E/S) ratio. Despite the prolonged cycle life, the combination of the interlayer and cellulose separator increases the polysulfide shuttle current, leading to reduced Coulombic efficiency. Based on XPS analyses, the latter is ascribed to a change in the composition of the solid electrolyte interphase (SEI) on Li. Meanwhile, electrolyte decomposition is found to be slower in cells with cellulose-based separators, which explains their longer cycle life. These counterintuitive observations demonstrate the complicated interactions between the cell components in the Li-S system and how strategies aiming to mitigate one unwanted process may exacerbate another. This study demonstrates the value of a holistic approach to the development of Li-S chemistry.<br>

Automatic quantitative analysis on mitochondrial morphology method

2010 ◽  
Vol 30 (11) ◽  
pp. 2991-2994
Author(s):  
Yong-ping WANG ◽  
Ke-di XU ◽  
Xiao-xiang ZHENG
Keyword(s):  
Quantitative Analysis ◽  
Mitochondrial Morphology

Thermodynamics of Flowing Systems: with Internal Microstructure

1994 ◽  
Author(s):  
Antony N. Beris ◽  
Brian J. Edwards
Keyword(s):  
Free Energy ◽  
Theoretical Study ◽  
Flow Behavior ◽  
Irreversible Thermodynamics ◽  
Hamiltonian Mechanics ◽  
Complex Interplay ◽  
Microscopic Models ◽  
Flow Phenomena ◽  
The Subject ◽  
The Continuum

This much-needed monograph presents a systematic, step-by-step approach to the continuum modeling of flow phenomena exhibited within materials endowed with a complex internal microstructure, such as polymers and liquid crystals. By combining the principles of Hamiltonian mechanics with those of irreversible thermodynamics, Antony N. Beris and Brian J. Edwards, renowned authorities on the subject, expertly describe the complex interplay between conservative and dissipative processes. Throughout the book, the authors emphasize the evaluation of the free energy--largely based on ideas from statistical mechanics--and how to fit the values of the phenomenological parameters against those of microscopic models. With Thermodynamics of Flowing Systems in hand, mathematicians, engineers, and physicists involved with the theoretical study of flow behavior in structurally complex media now have a superb, self-contained theoretical framework on which to base their modeling efforts.

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