scholarly journals The bHLH Transcription Factors TSAR1 and TSAR2 Regulate Triterpene Saponin Biosynthesis in Medicago truncatula

2015 ◽  
Vol 170 (1) ◽  
pp. 194-210 ◽  
Author(s):  
Jan Mertens ◽  
Jacob Pollier ◽  
Robin Vanden Bossche ◽  
Irene Lopez-Vidriero ◽  
José Manuel Franco-Zorrilla ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Medicago Truncatula ◽  
Triterpene Saponin ◽  
Saponin Biosynthesis ◽  
Bhlh Transcription Factors

scholarly journals ER-Anchored Transcription Factors bZIP17 and bZIP60 Regulate Triterpene Saponin Biosynthesis in Medicago truncatula1[OPEN]

2020 ◽  
Author(s):  
Bianca Ribeiro ◽  
Marie-Laure Erffelinck ◽  
Maite Colinas ◽  
Clara Williams ◽  
Evelien Van Hamme ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Er Stress ◽  
Indole Alkaloid ◽  
Stress Conditions ◽  
Basic Leucine Zipper ◽  
Defense Compounds ◽  
Model Legume ◽  
Triterpene Saponin ◽  
Saponin Biosynthesis ◽  
Bhlh Transcription Factors

ABSTRACTTriterpene saponins (TS) are a structurally diverse group of metabolites that are widely distributed in plants. They primarily serve as defense compounds and their production is often triggered by biotic stresses through signaling cascades that are modulated by phytohormones such as the jasmonates (JA). Two JA-modulated basic helix-loop-helix (bHLH) transcription factors (TFs), TRITERPENE SAPONIN BIOSYNTHESIS ACTIVATING REGULATOR 1 (TSAR1) and TSAR2, have been previously identified as direct activators of TS biosynthesis in the model legume Medicago truncatula. Here, we report on the involvement of the core endoplasmic reticulum (ER) stress basic leucine zipper (bZIP) TFs bZIP17 and bZIP60 in the regulation of TS biosynthesis. Expression and processing of M. truncatula bZIP17 and bZIP60 proteins was altered in roots with perturbed TS biosynthesis or treated with JA. Accordingly, such roots displayed an altered ER network structure. M. truncatula bZIP17 and bZIP60 proteins were shown to be capable of interfering with the TSAR-mediated transactivation of TS biosynthesis genes, particularly under ER stress conditions, when they translocate from the ER to the nucleus. Furthermore, the inhibitory role of ER stress bZIP TFs in the regulation of JA-dependent terpene biosynthetic pathways appears to be widespread in the plant kingdom, as we demonstrate that it also occurs in the regulation of monoterpene indole alkaloid biosynthesis in the medicinal plant Catharanthus roseus. We postulate that activation of ER stress bZIP TFs provides the plant with a mechanism to balance metabolic activities and thereby adequately govern modulation of growth, development and defense processes in defined stress situations.One sentence summaryER stress bZIP transcription factors can interfere with the activity of jasmonate-inducible bHLH transcription factors to modulate the elicitation of plant specialized metabolism in stress conditions.

scholarly journals Genomic and Coexpression Analyses Predict Multiple Genes Involved in Triterpene Saponin Biosynthesis in Medicago truncatula

2010 ◽  
Vol 22 (3) ◽  
pp. 850-866 ◽  
Author(s):  
Marina A. Naoumkina ◽  
Luzia V. Modolo ◽  
David V. Huhman ◽  
Ewa Urbanczyk-Wochniak ◽  
Yuhong Tang ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Triterpene Saponin ◽  
Saponin Biosynthesis ◽  
Multiple Genes

scholarly journals A genomics approach to the early stages of triterpene saponin biosynthesis in Medicago truncatula

2002 ◽  
Vol 32 (6) ◽  
pp. 1033-1048 ◽  
Author(s):  
Hideyuki Suzuki ◽  
Lahoucine Achnine ◽  
Ran Xu ◽  
Seiichi P. T. Matsuda ◽  
Richard A. Dixon
Keyword(s):  
Medicago Truncatula ◽  
Triterpene Saponin ◽  
Early Stages ◽  
Saponin Biosynthesis

scholarly journals A Seed-Specific Regulator of Triterpene Saponin Biosynthesis in Medicago truncatula

2020 ◽  
Vol 32 (6) ◽  
pp. 2020-2042 ◽  
Author(s):  
Bianca Ribeiro ◽  
Elia Lacchini ◽  
Keylla U. Bicalho ◽  
Jan Mertens ◽  
Philipp Arendt ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Triterpene Saponin ◽  
Saponin Biosynthesis

scholarly journals Insights into the Diversification of Subclade IVa bHLH Transcription Factors in Fabaceae

2020 ◽  
Author(s):  
Hayato Suzuki ◽  
Hikaru Seki ◽  
Toshiya Muranaka
Keyword(s):  
Transcription Factors ◽  
Expression Patterns ◽  
Lotus Japonicus ◽  
Helix Loop Helix ◽  
Saponin Biosynthesis ◽  
Plant Families ◽  
Almost All ◽  
Bhlh Transcription Factors ◽  
Bhlh Proteins ◽  
Group 1

Abstract Background: Fabaceae plants appear to contain larger numbers of subclade IVa basic-helix-loop-helix (bHLH) transcription factors than other plant families, and some members of this subclade have been identified as saponin biosynthesis regulators. We aimed to systematically elucidate the diversification of this subclade and obtain insights into the evolutionary history of saponin biosynthesis regulation in Fabaceae.Results: In this study, we collected sequences of subclade IVa bHLH proteins from 40 species, including fabids and other plants, and found greater numbers of subclade IVa bHLHs in Fabaceae. We confirmed conservation of the bHLH domain, C-terminal ACT-like domain, and exon-intron organisation among almost all subclade IVa members in model legumes, supporting the results of our classification. Phylogenetic tree-based classification of subclade IVa revealed the presence of three different groups. Interestingly, most Fabaceae subclade IVa bHLHs fell into group 1, which contained all legume saponin biosynthesis regulators identified to date. These observations support the co-occurrence and Fabaceae-specific diversification of saponin biosynthesis regulators. Comparing the expression of orthologous genes in Glycine max, Medicago truncatula, and Lotus japonicus, orthologues of MtTSAR1 (the first identified soyasaponin biosynthesis regulatory transcription factor) were not expressed in the same tissues, suggesting that group 1 members have gained different expression patterns and contributions to saponin biosynthesis during their duplication and divergence. On the other hand, groups 2 and 3 possessed fewer members, and their phylogenetic relationships and expression patterns were highly conserved, indicating that their activities may be conserved across Fabaceae.Conclusions: This study suggests subdivision and diversification of subclade IVa bHLHs in Fabaceae plants. The results will be useful for candidate selection of unidentified saponin biosynthesis regulators. Furthermore, the functions of groups 2 and 3 members are interesting targets for clarifying the evolution of subclade IVa bHLH transcription factors in Fabaceae.

scholarly journals Insights into the diversification of subclade IVa bHLH transcription factors in Fabaceae

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hayato Suzuki ◽  
Hikaru Seki ◽  
Toshiya Muranaka
Keyword(s):  
Transcription Factors ◽  
Expression Patterns ◽  
Lotus Japonicus ◽  
Helix Loop Helix ◽  
Saponin Biosynthesis ◽  
Plant Families ◽  
Almost All ◽  
Bhlh Transcription Factors ◽  
Bhlh Proteins ◽  
Group 1

Abstract Background Fabaceae plants appear to contain larger numbers of subclade IVa basic-helix-loop-helix (bHLH) transcription factors than other plant families, and some members of this subclade have been identified as saponin biosynthesis regulators. We aimed to systematically elucidate the diversification of this subclade and obtain insights into the evolutionary history of saponin biosynthesis regulation in Fabaceae. Results In this study, we collected sequences of subclade IVa bHLH proteins from 40 species, including fabids and other plants, and found greater numbers of subclade IVa bHLHs in Fabaceae. We confirmed conservation of the bHLH domain, C-terminal ACT-like domain, and exon-intron organisation among almost all subclade IVa members in model legumes, supporting the results of our classification. Phylogenetic tree-based classification of subclade IVa revealed the presence of three different groups. Interestingly, most Fabaceae subclade IVa bHLHs fell into group 1, which contained all legume saponin biosynthesis regulators identified to date. These observations support the co-occurrence and Fabaceae-specific diversification of saponin biosynthesis regulators. Comparing the expression of orthologous genes in Glycine max, Medicago truncatula, and Lotus japonicus, orthologues of MtTSAR1 (the first identified soyasaponin biosynthesis regulatory transcription factor) were not expressed in the same tissues, suggesting that group 1 members have gained different expression patterns and contributions to saponin biosynthesis during their duplication and divergence. On the other hand, groups 2 and 3 possessed fewer members, and their phylogenetic relationships and expression patterns were highly conserved, indicating that their activities may be conserved across Fabaceae. Conclusions This study suggests subdivision and diversification of subclade IVa bHLHs in Fabaceae plants. The results will be useful for candidate selection of unidentified saponin biosynthesis regulators. Furthermore, the functions of groups 2 and 3 members are interesting targets for clarifying the evolution of subclade IVa bHLH transcription factors in Fabaceae.

scholarly journals 1P123 DMA binding analyses of bHLH transcription factors by FRET measurements(Nucleic acid-binding proteins,Poster Presentations)

2007 ◽  
Vol 47 (supplement) ◽  
pp. S54
Author(s):  
Koji HASEGAWA ◽  
Tatsushi GOTO ◽  
Daisuke KITANO ◽  
Mari KOTOURA ◽  
Fumio TOKUNAGA ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Nucleic Acid ◽  
Binding Proteins ◽  
Nucleic Acid Binding ◽  
Nucleic Acid Binding Proteins ◽  
Poster Presentations ◽  
Bhlh Transcription Factors

Characterization of Transcription Factors Following Expression Profiling of Medicago truncatula–Botrytis spp. Interactions

2014 ◽  
Vol 32 (5) ◽  
pp. 1030-1040 ◽  
Author(s):  
Ángel M. Villegas-Fernández ◽  
Franziska Krajinski ◽  
Armin Schlereth ◽  
Eva Madrid ◽  
Diego Rubiales
Keyword(s):  
Transcription Factors ◽  
Medicago Truncatula ◽  
Expression Profiling
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