scholarly journals The Antarctic Psychrophile Chlamydomonas sp. UWO 241 Preferentially Phosphorylates a Photosystem I-Cytochrome b6/f Supercomplex

2015 ◽  
Vol 169 (1) ◽  
pp. 717-736 ◽  
Author(s):  
Beth Szyszka-Mroz ◽  
Paula Pittock ◽  
Alexander G. Ivanov ◽  
Gilles Lajoie ◽  
Norman P.A. Hüner
Keyword(s):  
Photosystem I ◽  
Cytochrome B6 ◽  
The Antarctic ◽  
Chlamydomonas Sp

scholarly journals Constitutively Up-regulated Carbon Metabolism is an Adaptation to Low Temperature in the Antarctic Psychrophile Chlamydomonas sp. UWO241

2021 ◽  
Author(s):  
Marina Cvetkovska ◽  
Beth Szyszka-Mroz ◽  
Nina Malczewski ◽  
David Smith ◽  
Norman P. A. Huner
Keyword(s):  
Growth Rate ◽  
Heat Stress ◽  
Steady State ◽  
Temperature Stress ◽  
Natural Habitat ◽  
Soluble Sugars ◽  
Physiological Characteristic ◽  
Rate Characteristic ◽  
The Antarctic ◽  
Chlamydomonas Sp

The Antarctic alga Chlamydomonas sp. UWO241 is an obligate psychrophile that thrives in the cold but is unable to survive at moderate, seemingly innocuous temperatures. We dissect the responses of UWO241 to temperature stress using global metabolomic approaches. UWO241 exhibits slow growth at 4°C, a temperature closest to its natural habitat, and faster growth at higher temperatures of 10-15°C. We demonstrate that the slower growth-rate characteristic of UWO241 at 4⁰C is not necessarily a hallmark of stress. UWO241 constitutively accumulates high levels of protective metabolites including soluble sugars, polyamines and antioxidants at a range of steady-state temperatures. In contrast, the mesophile Chlamydomonas reinhardtii accumulates these metabolites only during cold stress. Despite low growth rates, 4°C-grown UWO241 cultures had a higher capacity to respond to heat stress (24°C) and accumulated increased amounts of antioxidants, lipids and soluble sugars, when compared to cultures grown at 10-15°C. We conclude that the slower growth rate and the unique psychrophilic physiological characteristic of UWO241 grown at 4⁰C result in a permanently re-routed steady-state metabolism, which contributes to its increased resistance to heat stress. Our work adds to the growing body of research on temperature stress in psychrophiles, many of which are threatened by climate change.

scholarly journals Molecular cloning and functional analysis of a Δ12-fatty acid desaturase from the Antarctic microalga Chlamydomonas sp. ICE-L

3 Biotech ◽  
2019 ◽  
Vol 9 (9) ◽  
Author(s):  
Yingying He ◽  
Zhou Zheng ◽  
Meiling An ◽  
Hao Chen ◽  
Changfeng Qu ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Fatty Acid ◽  
Molecular Cloning ◽  
Fatty Acid Desaturase ◽  
The Antarctic ◽  
Chlamydomonas Sp

The Antarctic psychrophiles Chlamydomonas spp. UWO241 and ICE-MDV exhibit differential restructuring of photosystem I in response to iron

2019 ◽  
Vol 141 (2) ◽  
pp. 209-228 ◽  
Author(s):  
Greg Cook ◽  
Amber Teufel ◽  
Isha Kalra ◽  
Wei Li ◽  
Xin Wang ◽  
...  
Keyword(s):  
Photosystem I ◽  
The Antarctic

Molecular Cloning and Expression of a Cryptochrome Gene CiCRY-DASH1 from the Antarctic microalga Chlamydomonas sp. ICE-L

2019 ◽  
Vol 62 (2) ◽  
pp. 91-103
Author(s):  
Xin Zhang ◽  
Zhou Zheng ◽  
Yingying He ◽  
Lina Liu ◽  
Changfeng Qu ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Cloning And Expression ◽  
The Antarctic ◽  
Chlamydomonas Sp

Modulation of the Cytochrome b6/f Complex and Photosystem I Under Growth-Limiting Light in Amaranthus hypochondriacus, an NAD-ME C4 Plant

1996 ◽  
Vol 23 (3) ◽  
pp. 305 ◽  
Author(s):  
MV Sailaja ◽  
VSR Das
Keyword(s):  
Electron Transport ◽  
Photosystem Ii ◽  
Photosystem I ◽  
Thylakoid Membranes ◽  
Bundle Sheath ◽  
C4 Plant ◽  
Cytochrome B6 ◽  
Amaranthus Hypochondriacus ◽  
Energy Consuming ◽  
Electron Transport Rates

Highly characteristic responses of thylakoid membranes were observed in function and composition when fully developed plants of Amaranthus hypochondriacus L. grown under light sufficient (2000 μmol m-2 s-1) conditions were transferred to light limited conditions (650 μmol m-2 s-1 and 200 μmol m-2 s-1). The whole-chain, photosystem I and photosystem II electron transport rates were depressed in both bundle sheath and mesophyll thylakoids with remarkable differences between them in variation of rates under limiting light. The reduction in PSI electron transport in the mesophyll could be attributed to reduced PSI centres, while in the bundle sheath, a modulation of cytochrome b6/f complex regulated the rates of PSI electron transport. The requirement for an unaltered number of PSI centres under limiting light in the bundle sheath is ascribed to operation of an energy-consuming C4 pump.

Analysis of ΔpH and the xanthophyll cycle in NPQ of the Antarctic sea ice alga Chlamydomonas sp. ICE-L

Extremophiles ◽  
2013 ◽  
Vol 17 (3) ◽  
pp. 477-484 ◽  
Author(s):  
Shanli Mou ◽  
Xiaowen Zhang ◽  
Naihao Ye ◽  
Jinlai Miao ◽  
Shaona Cao ◽  
...  
Keyword(s):  
Sea Ice ◽  
Xanthophyll Cycle ◽  
Antarctic Sea Ice ◽  
The Antarctic ◽  
Chlamydomonas Sp

Photoaffinity Labeling of Spinach Thylakoids and Cytochrome b6/f-Complex by the Hydrophobic Reagent 3-(Trifluoromethyl)-3-(m-[125I]iodophenyl)-diazirine

1985 ◽  
Vol 40 (5-6) ◽  
pp. 454-456
Author(s):  
Walter Oettmeier ◽  
Hans-Joachim Soli ◽  
Ellen Olschewski
Keyword(s):  
Chlorophyll A ◽  
Cytochrome B ◽  
Photosystem I ◽  
Light Harvesting ◽  
Photoaffinity Labeling ◽  
Membrane System ◽  
Cytochrome B6 ◽  
Protein Components ◽  
Spinach Thylakoids

Abstract In isolated spinach thylakoids the hydrophobic photo­reactive probe 3-(trifluoromethyl)-3-(m-[125I]iodophenyl-diazirine almost exclusively labels the photosystem I reac­tion center and the light-harvesting chlorophyll a/b protein. In isolated cytochrome b6/f-complex, all four components of the complex get labeled, but to a different extent. The amount of labeling in the protein components is correlated to the number of polypeptide segments embedded in the membrane system.

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