Grapevine and Arabidopsis cation-chloride cotransporters localise to the Golgi and trans-Golgi network and indirectly influence long-distance ion homeostasis and plant salt tolerance

Phosphatidylserine Synthase From Salicornia Europaea Is Involved In Plant Salt Tolerance By Regulating Plasma Membrane Stability

Plant and Cell Physiology ◽

10.1093/pcp/pcaa141 ◽

2020 ◽

Author(s):

Sulian Lv ◽

Fang Tai ◽

Jie Guo ◽

Ping Jiang ◽

Kangqi Lin ◽

...

Keyword(s):

Plasma Membrane ◽

Salt Tolerance ◽

Ion Homeostasis ◽

Salicornia Europaea ◽

Suspension Cells ◽

Gene Encoding ◽

Membrane Injury ◽

Base Exchange ◽

Phosphatidylserine Synthase ◽

Plant Salt Tolerance

Abstract Salinity-induced lipid alterations have been reported in many plant species, however, how lipid biosynthesis and metabolism are regulated and how lipids work in plant salt tolerance are much less studied. Here a constitutively much higher phosphatidylserine (PS) content in plasma membrane (PM) was found in the euhalophyte Salicornia europaea than Arabidopsis. A gene encoding phosphatidylserine synthase (PSS) was subsequently isolated from S. europaea, named SePSS, which was induced by salinity. Multiple alignments and phylogenetic analysis suggested SePSS belong to base-exchange-type PSS, which locates in endoplasmic reticulum. Knockdown of SePSS in S. europaea suspension cells resulted in reduced PS content, decreased cell survival rate, increased PM depolarization and K+ efflux under 400 or 800 mM NaCl. By contrast, upregulation of SePSS leads to increased PS and phosphatidylethanolamine (PE) levels and enhanced salt tolerance in Arabidopsis, along with lower accumulation of reactive oxygen species, less membrane injury, less PM depolarization and higher K+/Na+ in the transgenic lines than WT. These results suggest the positive correlation between PS levels and plant salt tolerance, and SePSS participates in plant salt tolerance by regulating PS levels, hence PM potential and permeability, which help maintain ion homeostasis. Our work provides a potential strategy for improving plant growth under multiple stresses.

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ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network

The Journal of Cell Biology ◽

10.1083/jcb.201905097 ◽

2019 ◽

Vol 218 (11) ◽

pp. 3681-3696 ◽

Cited By ~ 4

Author(s):

Morié Ishida ◽

Juan S. Bonifacino

Keyword(s):

Coiled Coil ◽

Concerted Action ◽

Long Distance ◽

Master Regulator ◽

Trans Golgi Network ◽

Golgi Network

SNARE-mediated fusion of endosome-derived transport carriers with the trans-Golgi network (TGN) depends on the concerted action of two types of tethering factors: long coiled-coil tethers of the golgin family, and the heterotetrameric complex GARP. Whereas the golgins mediate long-distance capture of the carriers, GARP promotes assembly of the SNAREs. It remains to be determined, however, how the functions of these tethering factors are coordinated. Herein we report that the ARF-like (ARL) GTPase ARFRP1 functions upstream of two other ARL GTPases, ARL1 and ARL5, which in turn recruit golgins and GARP, respectively, to the TGN. We also show that this mechanism is essential for the delivery of retrograde cargos to the TGN. Our findings thus demonstrate that ARFRP1 is a master regulator of retrograde-carrier tethering to the TGN. The coordinated recruitment of distinct tethering factors by a bifurcated GTPase cascade may be paradigmatic of other vesicular fusion events within the cell.

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Improvement of Torenia fournieri salinity tolerance by expression of Arabidopsis AtNHX5

Functional Plant Biology ◽

10.1071/fp07269 ◽

2008 ◽

Vol 35 (3) ◽

pp. 185 ◽

Cited By ~ 12

Author(s):

Le-Yi Shi ◽

Hong-Qing Li ◽

Xiao-Ping Pan ◽

Guo-Jiang Wu ◽

Mei-Ru Li

Keyword(s):

Salt Stress ◽

Salt Tolerance ◽

Potential Role ◽

Ion Homeostasis ◽

Leaf Explants ◽

Wild Type ◽

Torenia Fournieri ◽

Regenerated Plants ◽

Plant Salt Tolerance

In this paper, transgenic torenia plants expressing the AtNHX5 gene from Arabidopsis in sense and antisense orientations were produced to examine the potential role of AtNHX5 in plant salt tolerance and development. We found that torenia plants overexpressing AtNHX5 showed markedly enhanced tolerance to salt stress compared with both wild-type and antisense AtNHX5 transgenic plants upon salt stress. Measurements of ion levels indicated that Na+ and K+ contents were all higher in AtNHX5 overexpressing shoots than in those of both wild-type and antisense AtNHX5 shoots treated with 50 mm NaCl. This indicated that overexpression of AtNHX5 could improve the salt tolerance of transgenic torenia via accumulation of both Na+ and K+ in shoots, in which overall ion homeostasis and osmotic adjustment was changed to sustain the increase in shoot salt tolerance. Further, we found that overexpression of AtNHX5 in torenia significantly improved the shoot regeneration frequency in leaf explants and increased the plantlet survival rate when transferring the regenerated plants to soil. In addition, the AtNHX5 expressing plants produced flowers earlier than both wild-type and the antisense AtNHX5 plants. Taken together, the results indicated that AtNHX5 functions not only in plant salt tolerance but also in plant growth and development.

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