scholarly journals Transcriptional Activity of the MADS Box ARLEQUIN/TOMATO AGAMOUS-LIKE1 Gene Is Required for Cuticle Development of Tomato Fruit

2015 ◽  
Vol 168 (3) ◽  
pp. 1036-1048 ◽  
Author(s):  
Estela Giménez ◽  
Eva Dominguez ◽  
Benito Pineda ◽  
Antonio Heredia ◽  
Vicente Moreno ◽  
...  
Keyword(s):  
Transcriptional Activity ◽  
Tomato Fruit ◽  
Mads Box ◽  
Cuticle Development

scholarly journals Early Cone Setting in Picea abies acrocona Is Associated with Increased Transcriptional Activity of a MADS Box Transcription Factor

2012 ◽  
Vol 161 (2) ◽  
pp. 813-823 ◽  
Author(s):  
Daniel Uddenberg ◽  
Johan Reimegård ◽  
David Clapham ◽  
Curt Almqvist ◽  
Sara von Arnold ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Picea Abies ◽  
Transcriptional Activity ◽  
Mads Box

scholarly journals The RIN-MC Fusion of MADS-Box Transcription Factors Has Transcriptional Activity and Modulates Expression of Many Ripening Genes

2017 ◽  
Vol 176 (1) ◽  
pp. 891-909 ◽  
Author(s):  
Shan Li ◽  
Huijinlan Xu ◽  
Zheng Ju ◽  
Dongyan Cao ◽  
Hongliang Zhu ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Transcriptional Activity ◽  
Mads Box ◽  
Ripening Genes

scholarly journals Characterization of a serum response factor-like protein in Saccharomyces cerevisiae, Rlm1, which has transcriptional activity regulated by the Mpk1 (Slt2) mitogen-activated protein kinase pathway.

1997 ◽  
Vol 17 (5) ◽  
pp. 2615-2623 ◽  
Author(s):  
Y Watanabe ◽  
G Takaesu ◽  
M Hagiwara ◽  
K Irie ◽  
K Matsumoto
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Kinase ◽  
Map Kinase ◽  
Transcriptional Activation ◽  
Transcriptional Activity ◽  
Mitogen Activated Protein Kinase ◽  
Dependent Manner ◽  
Mads Box ◽  
Mitogen Activated Protein

The Mpk1 (Slt2) mitogen-activated protein (MAP) kinase has been implicated in several biological processes in Saccharomyces cerevisiae. The Rlm1 protein, a member of the MADS box family of transcription factors, functions downstream of Mpk1 in the pathway. To characterize the role of Rlm1 in mediating the transcriptional activation by the Mpk1 pathway, we constructed a LexA-Rlm1 deltaN chimera in which sequences, including the MADS box domain of the Rlm1 protein, were replaced by the LexA DNA binding domain and tested the ability of this chimera to activate a LexA operator-controlled reporter gene. In this assay, the Rlm1 protein was found to activate transcription in a manner regulated by the Mpk1 pathway. The Mpk1 protein kinase phosphorylated Rlm1 deltaN in vitro and the LexA-Rlm1 deltaN chimera protein was phosphorylated in vivo in a Mpk1-dependent manner. These results suggest that Mpk1 regulates the transcriptional activity of Rlm1 by directly phosphorylating it. We identified a Mpk1-like protein kinase, Mlp1, as an Rlm1-associated protein by using the yeast two-hybrid system. Overexpression of MLP1 suppresses the caffeine-sensitive phenotype of the bck1 delta mutation. The additivity of the mlp1 delta defect with the Mpk1 delta defect with regard to the caffeine sensitivity, combined with the results of genetic epistasis experiments, suggested that the activity of Rlm1 is regulated independently by Mpk1 MAP kinase and the Mlp1 MAP kinase-like kinase.

scholarly journals The MADS-box transcription factor PHERES1 controls imprinting in the endosperm by binding to domesticated transposons

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Rita A Batista ◽  
Jordi Moreno-Romero ◽  
Yichun Qiu ◽  
Joram van Boven ◽  
Juan Santos-González ◽  
...  
Keyword(s):  
Binding Sites ◽  
Transcriptional Activity ◽  
Endosperm Development ◽  
Imprinted Genes ◽  
Mads Box ◽  
Binding Motifs ◽  
Molecular Domestication ◽  
Dna Binding Motifs ◽  
Carg Box ◽  
Eukaryotic Organisms

MADS-box transcription factors (TFs) are ubiquitous in eukaryotic organisms and play major roles during plant development. Nevertheless, their function in seed development remains largely unknown. Here, we show that the imprinted Arabidopsis thaliana MADS-box TF PHERES1 (PHE1) is a master regulator of paternally expressed imprinted genes, as well as of non-imprinted key regulators of endosperm development. PHE1 binding sites show distinct epigenetic modifications on maternal and paternal alleles, correlating with parental-specific transcriptional activity. Importantly, we show that the CArG-box-like DNA-binding motifs that are bound by PHE1 have been distributed by RC/Helitron transposable elements. Our data provide an example of the molecular domestication of these elements which, by distributing PHE1 binding sites throughout the genome, have facilitated the recruitment of crucial endosperm regulators into a single transcriptional network.

scholarly journals Interaction of two MADS-box genes leads to growth phenotype divergence of all-flesh type of tomatoes

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Baowen Huang ◽  
Guojian Hu ◽  
Keke Wang ◽  
Pierre Frasse ◽  
Elie Maza ◽  
...  
Keyword(s):  
Tomato Fruit ◽  
Fruit Softening ◽  
Gel Formation ◽  
Mads Box ◽  
Mads Box Genes ◽  
Commercial Use ◽  
Ripening Stages ◽  
Tomato Cultivars ◽  
Natural Mutation ◽  
Close Homolog

AbstractAll-flesh tomato cultivars are devoid of locular gel and exhibit enhanced firmness and improved postharvest storage. Here, we show that SlMBP3 is a master regulator of locular tissue in tomato fruit and that a deletion at the gene locus underpins the All-flesh trait. Intriguingly, All-flesh varieties lack the deleterious phenotypes reported previously for SlMBP3 under-expressing lines and which preclude any potential commercial use. We resolve the causal factor for this phenotypic divergence through the discovery of a natural mutation at the SlAGL11 locus, a close homolog of SlMBP3. Misexpressing SlMBP3 impairs locular gel formation through massive transcriptomic reprogramming at initial phases of fruit development. SlMBP3 influences locule gel formation by controlling cell cycle and cell expansion genes, indicating that important components of fruit softening are determined at early pre-ripening stages. Our findings define potential breeding targets for improved texture in tomato and possibly other fleshy fruits.

scholarly journals The Flowering Repressor SVP recruits the TOPLESS co-repressor to control flowering in chrysanthemum and Arabidopsis

2021 ◽  
Author(s):  
Jiafu Jiang ◽  
Zixin Zhang ◽  
Qian Hu ◽  
Yuqing Zhu ◽  
Zheng Gao ◽  
...  
Keyword(s):  
Transcriptional Activity ◽  
Dominant Negative ◽  
Mads Box ◽  
Loss Of Function ◽  
Vegetative Phase ◽  
Dominant Negative Mutation ◽  
Plant Life ◽  
Mads Box Gene ◽  
Floral Repressor ◽  
Transcriptional Complex

Plant flowering time is a consequence of the perception of environmental and endogenous signals. The MCM1-AGAMOUSDEFICIENS-SRF-box (MADS-box) gene SHORT VEGETATIVE PHASE (SVP) is a pivotal repressor that negatively regulates the floral transition during the vegetative phase. The transcriptional corepressor TOPLESS (TPL) plays critical roles in many aspects of plant life. An interaction first identified between the second LXLXLX motif (LRLGLP) of CmSVP with CmTPL1-2, which can repress the expression of a key flowering factor CmFTL3 by binding its promotor CArG element in chrysanthemum. Genetic analysis suggested that the CmSVP-CmTPL1-2 transcriptional complex is a prerequisite for SVP to act as a floral repressor, which reduces CmFTL3 transcriptional activity. CmSVP rescued the phenotype of the svp-31 mutant in Arabidopsis, and overexpression of AtSVP or CmSVP in the Arabidopsis dominant negative mutation tpl-1 led to a loss-of-function in late flowering, which confirmed the highly conserved function of SVP in the two completely different species. Thus, we have validated a conserved machinery wherein SVP relies on TPL to inhibit flowering through the direct regulation of FT, which is more meaningful for the evolution of species and could be translated to high-quality cultivation and breeding of crops.

scholarly journals The MADS-box transcription factor PHERES1 controls imprinting in the endosperm by binding to domesticated transposons

2019 ◽  
Author(s):  
Rita A. Batista ◽  
Jordi Moreno-Romero ◽  
Yichun Qiu ◽  
Joram van Boven ◽  
Juan Santos-González ◽  
...  
Keyword(s):  
Binding Sites ◽  
Transcriptional Activity ◽  
Endosperm Development ◽  
Imprinted Genes ◽  
Mads Box ◽  
Binding Motifs ◽  
Molecular Domestication ◽  
Dna Binding Motifs ◽  
Carg Box ◽  
Eukaryotic Organisms

AbstractMADS-box transcription factors are ubiquitous in eukaryotic organisms and play major roles during plant development. Nevertheless, their function in seed development remains largely unknown. Here we show that the imprinted Arabidopsis thaliana MADS-box TF PHERES1 (PHE1) is a master regulator of paternally expressed imprinted genes, as well as of non-imprinted key regulators of endosperm development. PHE1 binding sites show distinct epigenetic modifications on maternal and paternal alleles, correlating with parental-specific transcriptional activity. Importantly, we show that the CArG-box-like DNA-binding motifs bound by PHE1 have been distributed by RC/Helitron transposable elements. Our data provide an example of molecular domestication of these elements, which by distributing PHE1 binding sites throughout the genome, have facilitated the recruitment of crucial endosperm regulators into a single transcriptional network.

scholarly journals Understanding Cuticle Development in Tomato through the Study of Novel Germplasm with Malformed Cuticles

2013 ◽  
Author(s):  
Arthur A. Schaffer ◽  
Jocelyn Rose
Keyword(s):  
Tomato Fruit ◽  
Interspecific Crosses ◽  
Joint Project ◽  
Chemical Complexity ◽  
Wild Tomato Species ◽  
Tomato Species ◽  
Fruit Cracking ◽  
Cell Layers ◽  
The One ◽  
Cuticle Development

Plant cuticle development and metabolism are still poorly understood, partly due to the chemical complexity of the cuticular layer. The overall research objective was to broaden and deepen our understanding of tomato fruit cuticle development by analyzing novel germplasm with cuticular malformations and by studying the transcriptome and proteome of the fruit epidermal tissues, as strategies to overcome the challenges posed by the recalcitrance of the biological system. During the project we succeeded in identifying two genes with major impact on cuticle development. One of these encoded the first cutin synthase to be identified in plants, a metabolic step that had been a black box in cutin synthesis. In addition genes controlling the triterpenoid components of the cuticle were identified and, most interestingly, genetic variability for this component was identified among the wild tomato species germplasm. Additional germplasm was developed based on interspecific crosses that will allow for the future characterization of modifier genes that interact with the microfissuring gene (CWP) to promote or inhibit fruit cracking. One of the major accomplishments of the joint project was the integrated transcriptomic and proteomic analysis of the fruit cuticle and underlying tissues which allows for the identification of the pericarp cell layers responsible for the extracellular, cuticle-localized protein component. The results of the project have expanded our understanding of tomato fruit cuticle development and its genetic control. In addition, germplasm developed will be useful in developing tomato varieties resistant to cracking, on the one hand, and varieties useful for the dehydration industry on the other.  

scholarly journals GmFULc Is Induced by Short Days in Soybean and May Accelerate Flowering in Transgenic Arabidopsis thaliana

2021 ◽  
Vol 22 (19) ◽  
pp. 10333
Author(s):  
Jingzhe Sun ◽  
Mengyuan Wang ◽  
Chuanlin Zhao ◽  
Tianmeng Liu ◽  
Zhengya Liu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Flowering Time ◽  
Transcriptional Activity ◽  
Developmental Process ◽  
Transgenic Arabidopsis ◽  
Mads Box ◽  
Wild Type ◽  
Transcription Factor Family ◽  
Flowering Locus T ◽  
Short Days

Flowering is an important developmental process from vegetative to reproductive growth in plant; thus, it is necessary to analyze the genes involved in the regulation of flowering time. The MADS-box transcription factor family exists widely in plants and plays an important role in the regulation of flowering time. However, the molecular mechanism of GmFULc involved in the regulation of plant flowering is not very clear. In this study, GmFULc protein had a typical MADS domain and it was a member of MADS-box transcription factor family. The expression analysis revealed that GmFULc was induced by short days (SD) and regulated by the circadian clock. Compared to wild type (WT), overexpression of GmFULc in transgenic Arabidopsis caused significantly earlier flowering time, while ful mutants flowered later, and overexpression of GmFULc rescued the late-flowering phenotype of ful mutants. ChIP-seq of GmFULc binding sites identified potential direct targets, including TOPLESS (TPL), and it inhibited the transcriptional activity of TPL. In addition, the transcription levels of FLOWERING LOCUS T (FT), SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) and LEAFY (LFY) in the downstream of TPL were increased in GmFULc- overexpressionArabidopsis, suggesting that the early flowering phenotype was associated with up-regulation of these genes. Our results suggested that GmFULc inhibited the transcriptional activity of TPL and induced expression of FT, SOC1 and LFY to promote flowering.

Sign in / Sign up

Export Citation Format

Share Document