scholarly journals RNA Interference Knockdown of BRASSINOSTEROID INSENSITIVE1 in Maize Reveals Novel Functions for Brassinosteroid Signaling in Controlling Plant Architecture

2015 ◽  
Vol 169 (1) ◽  
pp. 826-839 ◽  
Author(s):  
Gokhan Kir ◽  
Huaxun Ye ◽  
Hilde Nelissen ◽  
Anjanasree K. Neelakandan ◽  
Andree S. Kusnandar ◽  
...  
Keyword(s):  
Rna Interference ◽  
Plant Architecture ◽  
Brassinosteroid Signaling

scholarly journals Brassinosteroid signaling integrates multiple pathways to release apical dominance in tomato

2021 ◽  
Vol 118 (11) ◽  
pp. e2004384118
Author(s):  
Xiaojian Xia ◽  
Han Dong ◽  
Yanling Yin ◽  
Xuewei Song ◽  
Xiaohua Gu ◽  
...  
Keyword(s):  
Regulatory Network ◽  
Apical Dominance ◽  
Plant Architecture ◽  
Response Regulator ◽  
Axillary Buds ◽  
Deficient Mutant ◽  
Type B ◽  
Apical Bud ◽  
Brassinosteroid Signaling ◽  
Bud Removal

The control of apical dominance involves auxin, strigolactones (SLs), cytokinins (CKs), and sugars, but the mechanistic controls of this regulatory network are not fully understood. Here, we show that brassinosteroid (BR) promotes bud outgrowth in tomato through the direct transcriptional regulation of BRANCHED1 (BRC1) by the BR signaling component BRASSINAZOLE-RESISTANT1 (BZR1). Attenuated responses to the removal of the apical bud, the inhibition of auxin, SLs or gibberellin synthesis, or treatment with CK and sucrose, were observed in bud outgrowth and the levels of BRC1 transcripts in the BR-deficient or bzr1 mutants. Furthermore, the accumulation of BR and the dephosphorylated form of BZR1 were increased by apical bud removal, inhibition of auxin, and SLs synthesis or treatment with CK and sucrose. These responses were decreased in the DELLA-deficient mutant. In addition, CK accumulation was inhibited by auxin and SLs, and decreased in the DELLA-deficient mutant, but it was increased in response to sucrose treatment. CK promoted BR synthesis in axillary buds through the action of the type-B response regulator, RR10. Our results demonstrate that BR signaling integrates multiple pathways that control shoot branching. Local BR signaling in axillary buds is therefore a potential target for shaping plant architecture.

ZmCLA4 regulates leaf angle through multiple plant hormone-mediated signal pathways in maize

2020 ◽  
Author(s):  
Dandan Dou ◽  
Shengbo Han ◽  
Lixia Ku ◽  
Huafeng Liu ◽  
Huihui Su ◽  
...  
Keyword(s):  
Regulatory Network ◽  
Plant Architecture ◽  
Plant Hormone ◽  
Auxin Transport ◽  
Regulatory Mechanism ◽  
Affinity Purification ◽  
Leaf Angle ◽  
Signal Pathways ◽  
Brassinosteroid Signaling ◽  
Ear Motif

AbstractLeaf angle in cereals is an important agronomic trait contributing to plant architecture and grain yield by determining the plant compactness. Although ZmCLA4 was identified to shape plant architecture by affecting leaf angle, the detailed regulatory mechanism of ZmCLA4 in maize remains unclear. ZmCLA4 was identified as a transcriptional repressor using the Gal4-LexA/UAS system and transactivation analysis in yeast. The DNA affinity purification (DAP)-seq assay showed that ZmCLA4 not only acts as a repressor containing the EAR motif (CACCGGAC), but was also found to have two new motifs, CCGARGS and CDTCNTC. On analyzing the ZmCLA4-bound targeted genes, we found that ZmCLA4, as a cross node of multiple plant hormone-mediated pathways, directly bound to ARF22 and IAA26 to regulate auxin transport and mediated brassinosteroid signaling by directly binding to BZR3 and 14-3-3. ZmCLA4 bound two WRKY genes involved with abscisic acid, two genes (CYP75B1, CYP93D1) involved with jasmonic acid, B3 involved in the response to ethylene, and thereby negatively regulated leaf angle formation. We built a new regulatory network for the ZmCLA4 gene controlling leaf angle in maize, which contributed to the understanding of ZmCLA4’s regulatory mechanism and will improve grain yields by facilitating optimization of plant architecture.

scholarly journals OsmiR396d Affects Gibberellin and Brassinosteroid Signaling to Regulate Plant Architecture in Rice

2017 ◽  
Vol 176 (1) ◽  
pp. 946-959 ◽  
Author(s):  
Yongyan Tang ◽  
Huanhuan Liu ◽  
Siyi Guo ◽  
Bo Wang ◽  
Zhitao Li ◽  
...  
Keyword(s):  
Plant Architecture ◽  
Brassinosteroid Signaling

Top Bending Panicle1 is involved in brassinosteroid signaling and regulates the plant architecture in rice

2017 ◽  
Vol 121 ◽  
pp. 1-13 ◽  
Author(s):  
Yun Lin ◽  
Zhigang Zhao ◽  
Shirong Zhou ◽  
Linglong Liu ◽  
Weiyi Kong ◽  
...  
Keyword(s):  
Plant Architecture ◽  
Brassinosteroid Signaling

Oryza sativa mediator subunit OsMED25 interacts with OsBZR1 to regulate brassinosteroid signaling and plant architecture in rice

2020 ◽  
Vol 62 (6) ◽  
pp. 793-811 ◽  
Author(s):  
Yuekun Ren ◽  
Xiaojie Tian ◽  
Shuyu Li ◽  
Enyang Mei ◽  
Mingliang He ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Plant Architecture ◽  
Brassinosteroid Signaling

scholarly journals Transcription Factor OsWRKY53 Positively Regulates Brassinosteroid Signaling and Plant Architecture

2017 ◽  
Vol 175 (3) ◽  
pp. 1337-1349 ◽  
Author(s):  
Xiaojie Tian ◽  
Xiufeng Li ◽  
Wenjia Zhou ◽  
Yuekun Ren ◽  
Zhenyu Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Plant Architecture ◽  
Brassinosteroid Signaling

scholarly journals OsOFP19 modulates plant architecture by integrating the cell division pattern and brassinosteroid signaling

2018 ◽  
Vol 93 (3) ◽  
pp. 489-501 ◽  
Author(s):  
Chao Yang ◽  
Yamei Ma ◽  
Yong He ◽  
Zhihong Tian ◽  
Jianxiong Li
Keyword(s):  
Cell Division ◽  
Plant Architecture ◽  
Division Pattern ◽  
Brassinosteroid Signaling

scholarly journals A BIN2-like1 Protein Confers Dwarf by Interacting with BZR1 of Brassinosteroid Signaling in Allotetraploid Brassica Napus

2021 ◽  
Author(s):  
Mao Yang ◽  
Jianbo He ◽  
Shubei Wan ◽  
Weiyan Li ◽  
Wenjing Chen ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Plant Height ◽  
Plant Architecture ◽  
Molecular Mechanisms ◽  
Glycogen Synthase ◽  
Molecular Evidence ◽  
Dwarf Phenotype ◽  
Brassinosteroid Signaling ◽  
Successive Generations

Abstract Brassinosteroids (BRs) are steroid hormones that play essential roles in plant growth and development. In this study, we identified a new dwarf mutant in Brassica napus. By map-based cloning, BnaC04.BIL1 (BnaC04g41660D) gene, a BIN2-like1 (BIL1) encoding a GLYCOGEN SYNTHASE KINASE 3 (GSK3-like) protein kinase, was isolated. To date, how BIL1 involves in BR signal transduction remains uncovered. Genetic transformation experiments confirmed that the BnaC04.BIL1 is responsible for the plant dwarf phenotype in the Bndwarf2 mutants. Overexpression of BnaC04.BIL1 not only reduced plant height, but also resulted in compact plant architecture. Using CRISPR/Cas9, two sgRNAs were designed to target BnaC04.BIL1 gene. The gene editing experiments generated mutations of BnaC04.BIL1 sequence, which were stably transmitted to successive generations, and lead to restoration of plant height and plant architecture. The molecular mechanism of Bndwarf2 dwarfing was further verified by Y2H and BiFC assays. Results shown that a Thr187Ser amino acid substitution residing in the conserved region promotes the interaction between BnaC04.BIL1Mut with BnaBZR1, thus enhances the negative regulation of plant growth. The genetic and molecular evidence clarifies first the BnaC04.BIL1 can sharply change plant architecture in natural plant accessions in allotetraploid, and provides new insights into the molecular mechanisms of BR signaling.

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