scholarly journals Down-Regulating CsHT1, a Cucumber Pollen-Specific Hexose Transporter, Inhibits Pollen Germination, Tube Growth, and Seed Development

2015 ◽  
Vol 168 (2) ◽  
pp. 635-647 ◽  
Author(s):  
Jintao Cheng ◽  
Zhenyu Wang ◽  
Fengzhen Yao ◽  
Lihong Gao ◽  
Si Ma ◽  
...  
Keyword(s):  
Seed Development ◽  
Pollen Germination ◽  
Tube Growth ◽  
Hexose Transporter ◽  
Germination Tube

scholarly journals Effects of Selected Fungicides on Pollen Germination, Tube Growth, and Distribution of the Cytoskeleton in Tradescantia virginiana

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 887E-887
Author(s):  
Yi He ◽  
Hazel Y. Wetzstein ◽  
Barrv A. Palevitz
Keyword(s):  
Light Microscopy ◽  
Pollen Germination ◽  
Cytoplasmic Streaming ◽  
Fruit Set ◽  
Plant Cells ◽  
Tube Growth ◽  
Inhibitory Effects ◽  
Higher Plant ◽  
Tradescantia Virginiana ◽  
Germination Tube

Fungicides have been shown to negatively affect pollen germination, tube growth, and fruit set in important crops. However, little is known regarding possible modes of action in higher plant cells. To address this, the effects of propiconazole or benomyl on pollen germination and tube growth were evaluated in Tradescantia virginiana using light microscopy and immunocytochemistry. Concentrations were selected at levels that had inhibitory effects, but did not totally arrest germination and tube elongation, i.e., propiconazole and benomyl were added at 0, 102, 136, or 170 μl·liter–1, and 0, 480, 600, or 720 mg·liter–1, respectively. Both fungicides inhibited germination, cytoplasmic streaming, tube elongation, and induced abnormal tube morphology and cytoskeletal distribution. Propiconazole-treated tubes had weaker microfilament signals, with amorphous staining. Microtubule (Mt) distribution was severely affected. In benomyl-treated tubes, Mts were fewer in number, fragmented, sinuous, and increasingly disorganized. Possible mechanism(s) will be discussed.

scholarly journals TESTING POTENTIAL ALTERNATE METHODS OF BLOOM THINNING FOR APPLE IN VITRO

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 892a-892
Author(s):  
Vikramjit Bajwa ◽  
Curt R. Rom
Keyword(s):  
Water Potential ◽  
Pollen Germination ◽  
Electrical Potential ◽  
Tube Growth ◽  
Metabolic Effects ◽  
In Vitro Tests ◽  
Chemical Solution ◽  
Solution Ph ◽  
Germination Tube

Alternate bloom thinners are needed for apple are needed to replace compounds which can no longer be used or have production system limitations. The effects of 24 chemicals selected as osmotic agents, organic acids, oils, essential oils, or potential metabolic agents and their properties of pH, electrical potential (EP) and water potential were tested in vitro on `Gala' apple pollen germination, tube growth and pistil damage. Solution concentrations of 0%, 0.25%, 0.5%, 1%, 2%, 5%, and 10% were prepared and solution pH, EP, and water potential measured. To test affects on germination, pollen was placed on agar germination media in petri dishes and then treated with 10: l of chemical solution. Percentage pollen germination and tube growth was calculated 4, 12, and 24 h after treatment. Excised pistils from forced flowers were placed on glass filter papers saturated with chemical solution. Pistil damage was visually, subjectively rated for damage indicated by discoloration 24 h after treatment. Effects of solution pH, EP and water potential on pollen germination, tube growth and pistil damage was significant with pH less than ∂4.0 or greater than ∂10.0, EP > 200mv, or water potential less than ∼4.0MPa inhibited pollen germination, growth, and killed pistils. Several chemical had apparent metabolic effects beyond the chemical effects mentioned above. In vitro tests were correlated to in vivo field tests in other studies indicating the use of pollen and pistil in vitro as a useful model for screening potential alternative thinning agents.

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