scholarly journals STATE TRANSITION7-Dependent Phosphorylation Is Modulated by Changing Environmental Conditions, and Its Absence Triggers Remodeling of Photosynthetic Protein Complexes

2015 ◽  
Vol 168 (2) ◽  
pp. 615-634 ◽  
Author(s):  
Sonja Verena Bergner ◽  
Martin Scholz ◽  
Kerstin Trompelt ◽  
Johannes Barth ◽  
Philipp Gäbelein ◽  
...  
Keyword(s):  
Environmental Conditions ◽  
Protein Complexes ◽  
Photosynthetic Protein Complexes

scholarly journals Composition, phosphorylation and dynamic organization of photosynthetic protein complexes in plant thylakoid membrane

2020 ◽  
Vol 19 (5) ◽  
pp. 604-619 ◽  
Author(s):  
Marjaana Rantala ◽  
Sanna Rantala ◽  
Eva-Mari Aro
Keyword(s):  
Thylakoid Membrane ◽  
Protein Complexes ◽  
Protein Network ◽  
Membrane Ultrastructure ◽  
Dynamic Organization ◽  
Photosynthetic Protein Complexes

Here we present an overview of the composition and organization of photosynthetic protein complexes in thylakoid membrane and discuss the consequences of the light-induced protein network re-organization to the thylakoid membrane ultrastructure.

scholarly journals Csde1 cooperates with Strap to control translation of erythroid transcripts

2017 ◽  
Author(s):  
Kat S. Moore ◽  
Nurcan Yagci ◽  
Floris van Alphen ◽  
Alexander B. Meijer ◽  
Peter A.C. ‘t Hoen ◽  
...  
Keyword(s):  
Growth Factors ◽  
Environmental Conditions ◽  
Rna Splicing ◽  
Translational Regulation ◽  
Rna Binding ◽  
Protein Complexes ◽  
Rna Binding Protein ◽  
Mrna Translation ◽  
Hematopoietic Progenitors ◽  
Control Translation

AbstractErythropoiesis is regulated at many levels, including control of mRNA translation. Changing environmental conditions, such as hypoxia, or the availability of nutrients and growth factors, require a rapid response enacted by the enhanced or repressed translation of existing transcripts. Csde1 is an RNA-binding protein required for erythropoiesis and strongly upregulated in erythroblasts relative to other hematopoietic progenitors. The aim of this study is to identify the Csde1-containing protein complexes, and investigate their role in regulating the translation of Csde1-bound transcripts. We show that Strap, also called Unrip, was the protein most strongly associated with Csde1 in erythroblasts. Strap is a WD40 protein involved in signaling and RNA splicing, but its role is unknown when associated with Csde1. Reduced expression of Strap did not alter the pool of transcripts bound by Csde1. Instead, it reduced the mRNA and/or protein expression of several Csde1-bound transcript, that encode for proteins essential for translational regulation during hypoxia, such as Hmbs, eIF4g3 and Pabpc4. Also affected by Strap knockdown were Vim, a Gata-1 target crucial for erythrocyte enucleation, and Elavl1, which stabilizes Gata-1 mRNA. Thus, we found that the Csde1/Strap complex is at the crossroad of multiple pathways governing translation in erythroblasts.

scholarly journals Atomic force microscopy visualizes mobility of photosynthetic proteins in grana thylakoid membranes

2018 ◽  
Author(s):  
Bibiana Onoa ◽  
Shingo Fukuda ◽  
Masakazu Iwai ◽  
Carlos Bustamante ◽  
Krishna K. Niyogi
Keyword(s):  
High Speed ◽  
Spinacia Oleracea ◽  
Protein Complexes ◽  
Rotational Diffusion ◽  
Thylakoid Membranes ◽  
Spatiotemporal Resolution ◽  
Force Microscopy ◽  
Photosynthetic Complexes ◽  
Protein Density ◽  
Photosynthetic Protein Complexes

ABSTRACTThylakoid membranes in chloroplasts contain photosynthetic protein complexes that convert light energy into chemical energy. Photosynthetic protein complexes are considered to undergo structural reorganization to maintain the efficiency of photochemical reactions. A detailed description of the mobility of photosynthetic complexes in real-time is necessary to understand how macromolecular organization of the membrane is altered by environmental fluctuations. Here, we used high-speed atomic force microscopy to visualize and characterize the in situ mobility of individual protein complexes in grana thylakoid membranes isolated from Spinacia oleracea. Our observations reveal that these membranes can harbor complexes with at least two distinctive classes of mobility. A large fraction of grana membranes contained proteins with quasi-static mobility, exhibiting molecular displacements smaller than 10 nm2. In the remaining fraction, the protein mobility is variable with molecular displacements of up to 100 nm2. This visualization at high-spatiotemporal resolution enabled us to estimate an average diffusion coefficient of ∼1 nm2 s-1. Interestingly, both confined and Brownian diffusion models could describe the protein mobility of the second group of membranes. We also provide the first direct evidence of rotational diffusion of photosynthetic complexes. The rotational diffusion of photosynthetic complexes could be an adaptive response to the high protein density in the membrane to guarantee the efficiency of electron transfer reactions. This characterization of the mobility of individual photosynthetic complexes in grana membranes establishes a foundation that could be adapted to study the dynamics of the complexes inside the intact and photosynthetically functional thylakoid membranes to be able to understand its structural responses to diverse environmental fluctuations.STATEMENT OF SIGNIFICANCEWe characterized the dynamics of individual photosynthetic protein complexes in grana thylakoid membranes from Spinacia oleracea by high-speed atomic microscopy (HS-AFM). Direct visualization at high spatiotemporal resolution unveils that the mobility of photosynthetic proteins is heterogeneous but governed by the confinement effect imposed by the high protein density in the thylakoid membrane. The photosynthetic complexes display rotational diffusion, which might be a consequence of the crowded environment in the membrane and a mechanism to sustain an efficient electron transfer chain.

scholarly journals Ultrafast Dynamics at 25T in Photosynthetic Protein Complexes

2016 ◽  
Author(s):  
M. Maiuri ◽  
M. B. Oviedo ◽  
J. C. Dean ◽  
Z. S. D. Toa ◽  
M. Bishop ◽  
...  
Keyword(s):  
Protein Complexes ◽  
Ultrafast Dynamics ◽  
Photosynthetic Protein Complexes

Spin label EPR study of lipid solvation of supramolecular photosynthetic protein complexes in thylakoids

1994 ◽  
Vol 1196 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Anabella Ivancich ◽  
LászlóI. Horváth ◽  
Magdolna Droppa ◽  
Gábor Horváth ◽  
Tibor Farkas
Keyword(s):  
Spin Label ◽  
Protein Complexes ◽  
Photosynthetic Protein Complexes

Photosynthesis gene superoperons in purple nonsulfur bacteria: the tip of the iceberg?

1992 ◽  
Vol 38 (1) ◽  
pp. 20-27 ◽  
Author(s):  
C. L. Wellington ◽  
C. E. Bauer ◽  
J. T. Beatty
Keyword(s):  
Key Words ◽  
Environmental Conditions ◽  
Rhodobacter Capsulatus ◽  
Protein Complexes ◽  
Photosynthetic Pigment ◽  
Purple Nonsulfur Bacteria ◽  
Pigment Protein Complexes ◽  
Photosynthesis Genes ◽  
Overlapping Transcription ◽  
Photosynthesis Gene

The purple, nonsulfur phototrophic bacterium Rhodobacter capsulatus has recently been found to contain several pigment biosynthetic operons that exhibit marked transcription read through into downstream operons that encode polypeptide components of photosynthetic pigment-protein complexes. This phenomenon has been found to be phenotypically significant for adaptation to changes in environmental conditions, and the term superoperon has been proposed to describe this sort of coupled transcriptional arrangement. We summarize the data that led to recognition of this novel transcriptional arrangement and suggest that superoperons might be more prevalent in prokaryotes than has heretofore been recognized. Key words: superoperons, overlapping transcription units, photosynthesis genes.

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