scholarly journals Pollen Tubes of Nicotiana alata Express Two Genes from Different β-Glucan Synthase Families

2001 ◽  
Vol 125 (4) ◽  
pp. 2040-2052 ◽  
Author(s):  
Monika S. Doblin ◽  
Linda De Melis ◽  
Ed Newbigin ◽  
Antony Bacic ◽  
Steve M. Read
Keyword(s):  
Pollen Tubes ◽  
Nicotiana Alata ◽  
Glucan Synthase

Molecular control of the glucan synthase-like protein NaGSL1 and callose synthesis during growth of Nicotiana alata pollen tubes

2008 ◽  
Vol 414 (1) ◽  
pp. 43-52 ◽  
Author(s):  
Lynette Brownfield ◽  
Sarah Wilson ◽  
Ed Newbigin ◽  
Antony Bacic ◽  
Steve Read
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Nicotiana Alata ◽  
Molecular Control ◽  
Intracellular Location ◽  
Glucan Synthase ◽  
Tobacco Pollen

The protein NaGSL1 (Nicotiana alata glucan synthase-like 1) is implicated in the synthesis of callose, the 1,3-β-glucan that is the major polysaccharide in the walls of N. alata (flowering tobacco) pollen tubes. Here we examine the production, intracellular location and post-translational processing of NaGSL1, and relate each of these to the control of pollen-tube callose synthase (CalS). The 220 kDa NaGSL1 polypeptide is produced after pollen-tube germination and accumulates during pollen-tube growth, as does CalS. A combination of membrane fractionation and immunoelectron microscopy revealed that NaGSL1 was present predominantly in the endoplasmic reticulum and Golgi membranes in younger pollen tubes when CalS was mostly in an inactive (latent) form. In later stages of pollen-tube growth, when CalS was present in both latent and active forms, a greater proportion of NaGSL1 was in intracellular vesicles and the plasma membrane, the latter location being consistent with direct deposition of callose into the wall. N. alata CalS is activated in vitro by the proteolytic enzyme trypsin and the detergent CHAPS, but in neither case was activation associated with a detectable change in the molecular mass of the NaGSL1 polypeptide. NaGSL1 may thus either be activated by the removal of a few amino acids or by the removal of another protein that inhibits NaGSL1. These findings are discussed in relation to the control of callose biosynthesis during pollen germination and pollen-tube growth.

scholarly journals Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes.

1991 ◽  
pp. 271-283 ◽  
Author(s):  
J. E. Gray ◽  
B. A. McClure ◽  
I. Bonig ◽  
M. A. Anderson ◽  
A. E. Clarke
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Nicotiana Alata ◽  
Self Incompatibility

An in vitro assay for assessing the effects of growth factors on Nicotiana alata pollen tubes

1997 ◽  
Vol 10 (6) ◽  
pp. 351-357 ◽  
Author(s):  
W. M. Lush ◽  
A. S. Opat ◽  
F. Nie ◽  
Adrienne E. Clarke
Keyword(s):  
Growth Factors ◽  
Pollen Tubes ◽  
In Vitro Assay ◽  
Nicotiana Alata ◽  
Vitro Assay

Role of a callose synthase zymogen in regulating wall deposition in pollen tubes of Nicotiana alata Link et Otto

Planta ◽  
1999 ◽  
Vol 208 (4) ◽  
pp. 528-538 ◽  
Author(s):  
Huijuan Li ◽  
Antony Bacic ◽  
Steve M. Read
Keyword(s):  
Pollen Tubes ◽  
Nicotiana Alata ◽  
Callose Synthase ◽  
Wall Deposition

Membrane fractionation and enrichment of callose synthase from pollen tubes of Nicotiana alata Link et Otto

Planta ◽  
1998 ◽  
Vol 205 (3) ◽  
pp. 380-388 ◽  
Author(s):  
Adrian Turner ◽  
Antony Bacic ◽  
Philip J. Harris ◽  
Steve M. Read
Keyword(s):  
Pollen Tubes ◽  
Nicotiana Alata ◽  
Callose Synthase ◽  
Membrane Fractionation

Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes

1991 ◽  
Vol 3 (3) ◽  
pp. 271 ◽  
Author(s):  
Julie E. Gray ◽  
Bruce A. McClure ◽  
Ingrid Bonig ◽  
Marilyn A. Anderson ◽  
Adrienne E. Clarke
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Nicotiana Alata ◽  
Self Incompatibility

scholarly journals In Vitro Grown Pollen Tubes of Nicotiana alata Actively Synthesise a Fucosylated Xyloglucan

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e77140 ◽  
Author(s):  
Edwin R. Lampugnani ◽  
Isabel E. Moller ◽  
Andrew Cassin ◽  
Daniel F. Jones ◽  
Poh Ling Koh ◽  
...  
Keyword(s):  
Pollen Tubes ◽  
Nicotiana Alata

Facts and hypotheses on the origin of S mutations and on the function of the S gene in Nicotiana alata and Lycopersicum peruvianum

1975 ◽  
Vol 188 (1092) ◽  
pp. 345-360 ◽  
Keyword(s):  
Electron Microscopy ◽  
Protein Synthesis ◽  
Tandem Repeats ◽  
Pollen Tubes ◽  
Nicotiana Alata ◽  
S Locus ◽  
Interspecific Incompatibility ◽  
S Gene ◽  
Giberellic Acid ◽  
Incompatibility Reactions

Fragments in certain pollen-part mutants are suspected to originate from duplications of satellited regions. Linkage tests demonstrate that, if this interpretation is correct, either the specificity segment of the S locus is not on the fragment or two unliked specificity segments operate respectively in pollen and style. As electron microscopy and the effects of giberellic acid on selfing suggest that one manifestation of incompatibility reactions is a cessation of protein synthesis in pollen tubes, it is possible that the fragment does not carry an S locus but ribosomal cistrons necessary for the maintenance of activity. Ancestry tests are under way for testing the hypothesis that different specificity segments are organized as tandem repeats and can be switched on and off in inbred backgrounds. Electron microscopy analyses of self-incompatible hybrids indicate that a single gametophytic factor governs interspecific incompatibility in hybrid pollen and is allelic or linked to the S locus.

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