scholarly journals Molecular and Biochemical Characterization of the Involvement of Cyclin-Dependent Kinase A during the Early Development of Tomato Fruit

1999 ◽  
Vol 121 (3) ◽  
pp. 857-869 ◽  
Author(s):  
Jérôme Joubès ◽  
Thi-Hai Phan ◽  
Daniel Just ◽  
Christophe Rothan ◽  
Catherine Bergounioux ◽  
...  
Keyword(s):  
Early Development ◽  
Biochemical Characterization ◽  
Tomato Fruit ◽  
Cyclin Dependent Kinase ◽  
Kinase A

scholarly journals Biochemical Characterization of Pantoate Kinase, a Novel Enzyme Necessary for Coenzyme A Biosynthesis in the Archaea

2012 ◽  
Vol 194 (19) ◽  
pp. 5434-5443 ◽  
Author(s):  
H. Tomita ◽  
Y. Yokooji ◽  
T. Ishibashi ◽  
T. Imanaka ◽  
H. Atomi
Keyword(s):  
Coenzyme A ◽  
Biochemical Characterization ◽  
Coenzyme A Biosynthesis ◽  
Kinase A

Molecular characterization of the expression of distinct classes of cyclins during the early development of tomato fruit

Planta ◽  
2000 ◽  
Vol 211 (3) ◽  
pp. 430-439 ◽  
Author(s):  
Jérôme Joubès ◽  
David Walsh ◽  
Philippe Raymond ◽  
Christian Chevalier
Keyword(s):  
Molecular Characterization ◽  
Early Development ◽  
Tomato Fruit

scholarly journals Biochemical Characterization of the Drosophila Wingless Signaling Pathway Based on RNA Interference

2004 ◽  
Vol 24 (5) ◽  
pp. 2012-2024 ◽  
Author(s):  
Hiroko Matsubayashi ◽  
Sonoka Sese ◽  
Jong-Seo Lee ◽  
Tadaoki Shirakawa ◽  
Takeshi Iwatsubo ◽  
...  
Keyword(s):  
Rna Interference ◽  
Biochemical Characterization ◽  
The Other ◽  
Protein Levels ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Mediated Priming ◽  
Kinase A

ABSTRACT Regulation of Armadillo (Arm) protein levels through ubiquitin-mediated degradation plays a central role in the Wingless (Wg) signaling. Although zeste-white3 (Zw3)-mediated Arm phosphorylation has been implicated in its degradation, we have recently shown that casein kinase Iα (CKIα) also phosphorylates Arm and induces its degradation. However, it remains unclear how CKIα and Zw3, as well as other components of the Arm degradation complex, regulate Arm phosphorylation in response to Wg. In particular, whether Wg signaling suppresses CKIα- or Zw3-mediated Arm phosphorylaytion in vivo is unknown. To clarify these issues, we performed a series of RNA interference (RNAi)-based analyses in Drosophila S2R+ cells by using antibodies that specifically recognize Arm phosphorylated at different serine residues. These analyses revealed that Arm phosphorylation at serine-56 and at threonine-52, serine-48, and serine-44, is mediated by CKIα and Zw3, respectively, and that Zw3-directed Arm phosphorylation requires CKIα-mediated priming phosphorylation. Daxin stimulates Zw3- but not CKIα-mediated Arm phosphorylation. Wg suppresses Zw3- but not CKIα-mediated Arm phosphorylation, indicating that a vital regulatory step in Wg signaling is Zw3-mediated Arm phosphorylation. In addition, further RNAi-based analyses of the other aspects of the Wg pathway clarified that Wg-induced Dishevelled phosphoylation is due to CKIα and that presenilin and protein kinase A play little part in the regulation of Arm protein levels in Drosophila tissue culture cells.

scholarly journals In Silico Characterization of a Cyclin Dependent Kinase -A (CDKA) and its Coding Gene in some Oryza Species

2020 ◽  
Vol 17 (3) ◽  
pp. 0760
Author(s):  
Ahmed Sayed Fouad ◽  
Sanad M AlSobeai
Keyword(s):  
Binding Site ◽  
In Silico ◽  
Oryza Species ◽  
Regulatory Subunit ◽  
Binding Motif ◽  
World Population ◽  
Cyclin Dependent Kinase ◽  
Molecular Approaches ◽  
Kinase A

Rice (Oryza sativa) is a fundamental food for the majority of world population. Cyclin Dependent Kinase -A (CDKA) accelerates transition through different stages of cell cycle and contributes in gametes formation. In the present investigation, a CDKA encoding gene along with the corresponding protein were characterized in O. sativa Indica Group, O. glaberrima,  O. barthii, O. brachyantha, O. glumipatula, O. longistaminata, O. meridionalis, O. nivara, O. punctata and O. rufipogon using in silico analyses. The results reflected little variation in most species except O. longistaminata and O. brachyantha. Compared with the remaining species, O. longistaminata lacked a negative regulatory binding site and had a modified cyclin binding site (PSTAICE instead of PSTAIRE) that may lead to future characterization of a new distinct subclass of CDKAs. O. brachyantha had a modified SUC/CKS (suppressor of CDC2/cyclin dependent-kinase regulatory subunit)-binding motif. The observed variations can be exploited through traditional breeding or molecular approaches to manipulate cell division and growth of cultivated Oryza species.

Expression and biochemical characterization of the Plasmodium falciparum protein kinase A catalytic subunit

2009 ◽  
Vol 104 (6) ◽  
pp. 1299-1305 ◽  
Author(s):  
Nathalie Wurtz ◽  
Boris Pastorino ◽  
Lionel Almeras ◽  
Sébastien Briolant ◽  
Claude Villard ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Biochemical Characterization ◽  
Catalytic Subunit ◽  
Falciparum Protein ◽  
Kinase A

scholarly journals Biochemical Characterization of Lectin Isolated from Cherry Tomato Fruit

2007 ◽  
Vol 17 (2) ◽  
pp. 254-259 ◽  
Author(s):  
Na-Young Park ◽  
Sam-Pin Lee ◽  
Kwang-Soo Roh
Keyword(s):  
Biochemical Characterization ◽  
Tomato Fruit ◽  
Cherry Tomato

Biochemical characterization of highly active Trypanosoma brucei gambiense glycerol kinase, a promising drug target

2013 ◽  
Vol 154 (1) ◽  
pp. 77-84 ◽  
Author(s):  
E. O. Balogun ◽  
D. K. Inaoka ◽  
T. Shiba ◽  
Y. Kido ◽  
T. Nara ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Drug Target ◽  
Biochemical Characterization ◽  
Glycerol Kinase ◽  
Trypanosoma Brucei Gambiense ◽  
Highly Active ◽  
Kinase A

Plant Pathology Diagnosed by X-Ray Microanalysis

1987 ◽  
Vol 45 ◽  
pp. 974-975
Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang
Keyword(s):  
Electron Microscopy ◽  
Plant Pathology ◽  
Biochemical Characterization ◽  
Nutrient Deficiency ◽  
Green Leaf ◽  
Parasitic Infestation ◽  
X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

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