scholarly journals Reduced Activity of Geranylgeranyl Reductase Leads to Loss of Chlorophyll and Tocopherol and to Partially Geranylgeranylated Chlorophyll in Transgenic Tobacco Plants Expressing Antisense RNA for Geranylgeranyl Reductase

1999 ◽  
Vol 120 (3) ◽  
pp. 695-704 ◽  
Author(s):  
Ryouichi Tanaka ◽  
Ulrike Oster ◽  
Elisabeth Kruse ◽  
Wolfhart Rüdiger ◽  
Bernhard Grimm
Keyword(s):  
Transgenic Tobacco ◽  
Antisense Rna ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Reduced Activity

Viral Protection in Transgenic Tobacco Plants Expressing the Cucumber Mosaic Virus Coat Protein or its Antisense RNA

1988 ◽  
Vol 6 (5) ◽  
pp. 549-557 ◽  
Author(s):  
Maria Cuozzo ◽  
Keith M. O'Connell ◽  
Wojciech Kaniewski ◽  
Rong-Xiang Fang ◽  
Nam-Hai Chua ◽  
...  
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
Antisense Rna ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals Reduction of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Content by Antisense RNA Reduces Photosynthesis in Transgenic Tobacco Plants

1992 ◽  
Vol 98 (1) ◽  
pp. 294-302 ◽  
Author(s):  
Graham S. Hudson ◽  
John R. Evans ◽  
Susanne von Caemmerer ◽  
Yvonne B. C. Arvidsson ◽  
T. John Andrews
Keyword(s):  
Transgenic Tobacco ◽  
Antisense Rna ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Bisphosphate Carboxylase

Antisense RNA inhibition of ?-glucuronidase gene expression in transgenic tobacco plants

1989 ◽  
Vol 13 (4) ◽  
pp. 399-409 ◽  
Author(s):  
Laurian S. Robert ◽  
Pauline A. Donaldson ◽  
Christine Ladaique ◽  
Illimar Altosaar ◽  
Paul G. Arnison ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Tobacco ◽  
Antisense Rna ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Rna Inhibition

Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells

2005 ◽  
Vol 14 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Helga Schinkel ◽  
Andreas Schiermeyer ◽  
Raphael Soeur ◽  
Rainer Fischer ◽  
Stefan Schillberg
Keyword(s):  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Suspension Cells ◽  
Tobacco Plants ◽  
Recombinant Thrombomodulin

scholarly journals Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

2021 ◽  
Author(s):  
Ai-Hua Wang ◽  
Lan Yang ◽  
Xin-Zhuan Yao ◽  
Xiao-Peng Wen
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

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