scholarly journals A Gene Encoding Proline Dehydrogenase Is Not Only Induced by Proline and Hypoosmolarity, but Is Also Developmentally Regulated in the Reproductive Organs of Arabidopsis

1998 ◽  
Vol 118 (4) ◽  
pp. 1233-1241 ◽  
Author(s):  
Kazuo Nakashima ◽  
Rie Satoh ◽  
Tomohiro Kiyosue ◽  
Kazuko Yamaguchi-Shinozaki ◽  
Kazuo Shinozaki
Keyword(s):  
Reproductive Organs ◽  
Proline Dehydrogenase ◽  
Developmentally Regulated ◽  
Gene Encoding

scholarly journals Dissecting the sugarcane expressed sequence tag (SUCEST) database: unraveling flower-specific genes

2001 ◽  
Vol 24 (1-4) ◽  
pp. 77-84 ◽  
Author(s):  
R.C. Figueiredo ◽  
M.S. Brito ◽  
L.H.M. Figueiredo ◽  
A.C. Quiapin ◽  
P.M. Vitorelli ◽  
...  
Keyword(s):  
Expressed Sequence Tag ◽  
Tissue Expression ◽  
Reproductive Organs ◽  
Cdna Libraries ◽  
Computer Assisted ◽  
Transcript Accumulation ◽  
Gene Encoding ◽  
Assisted Analysis ◽  
Expressed Sequence ◽  
Different Tissues

There are almost 260,000 independent clones sequenced from the 5’ end in the Sugarcane Expressed Sequence Tag (SUCEST) database, which have been obtained from 37 cDNA libraries prepared from different tissues. This large number of expressed sequence tags (ESTs) provides an opportunity, unprecedented in plants, to perform ‘digital differential screening’ on selected cDNA libraries. In general, the frequency of a particular EST correlates with transcript accumulation in the tissues from which the cDNA libraries were constructed, so it is possible to compare the whole transcriptome from different tissues using computer-assisted analysis of an EST database. In our research we analyzed sugarcane ESTs according to tissue expression and identified more than 1,000 putative flower-specific genes. The fact that using this technique we were able to identify sugarcane homologues of several genes previously described as pollen-specific justifies this method of assessing tissue specificity. In addition, ESTs similar to genes specific to reproductive organs were detected e.g. a sugarcane gene encoding a meiotic protein essential for assembly of the synaptonemal complex and normal synapsis. This approach also allowed the identification of many flower-specific anonymous sequences that are good candidates for being novel genes involved in plant reproduction. This paper describes the analysis of the gene expression levels of 24 EST clusters during flower development using a ‘digital northern blot’ constructed from direct EST counts made on the non-normalized sugarcane cDNA libraries.

scholarly journals Expression of a Gene Encoding Trypanosoma congolense Putative Abc1 Family Protein is Developmentally Regulated

2005 ◽  
Vol 67 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Waren N. BATICADOS ◽  
William H. WITOLA ◽  
Noboru INOUE ◽  
Jung-Yeon, KIM ◽  
Noritaka KUBOKI ◽  
...  
Keyword(s):  
Trypanosoma Congolense ◽  
Developmentally Regulated ◽  
Gene Encoding ◽  
Family Protein

The developmentally regulated ECM glycoprotein ISG plays an essential role in organizing the ECM and orienting the cells of Volvox

2000 ◽  
Vol 113 (24) ◽  
pp. 4605-4617
Author(s):  
A. Hallmann ◽  
D.L. Kirk
Keyword(s):  
Critical Role ◽  
Somatic Cells ◽  
Cell Types ◽  
Model System ◽  
Swimming Behavior ◽  
Truncated Form ◽  
Developmentally Regulated ◽  
Gene Encoding ◽  
Ecm Architecture ◽  
Multicellular Organisms

Volvox is one of the simplest multicellular organisms with only two cell types, yet it has a surprisingly complex extracellular matrix (ECM) containing many region-specific morphological components, making Volvox suitable as a model system for ECM investigations. ECM deposition begins shortly after inversion, which is the process by which the embryo turns itself right-side-out at the end of embryogenesis. It was previously shown that the gene encoding an ECM glycoprotein called ISG is transcribed very transiently during inversion. Here we show that the developmentally controlled ISG accumulates at the bases of the flagella right after inversion, before any morphologically recognizable ECM structures have yet developed. Later, ISG is abundant in the ‘flagellar hillocks’ that encircle the basal ends of all flagella, and in the adjacent ‘boundary zone’ that delimits the spheroid. Transgenic Volvox were generated which express a truncated form of ISG. These transgenics exhibit a severely disorganized ECM within which the cells are embedded in a highly chaotic manner that precludes motility. A synthetic version of the C-terminal decapeptide of ISG has a similar disorganizing effect, but only when it is applied during or shortly after inversion. We postulate that ISG plays a critical role in morphogenesis and acts as a key organizer of ECM architecture; at the very beginning of ECM formation ISG establishes an essential initial framework that both holds the somatic cells in an adaptive orientation and acts as the scaffold upon which the rest of the ECM can be properly assembled, assuring that somatic cells of post-inversion spheroids are held in orientations and locations that makes adaptive swimming behavior possible.

scholarly journals ACTCAT, a Novel cis-Acting Element for Proline- and Hypoosmolarity-Responsive Expression of the ProDH Gene Encoding Proline Dehydrogenase in Arabidopsis

2002 ◽  
Vol 130 (2) ◽  
pp. 709-719 ◽  
Author(s):  
Rie Satoh ◽  
Kazuo Nakashima ◽  
Motoaki Seki ◽  
Kazuo Shinozaki ◽  
Kazuko Yamaguchi-Shinozaki
Keyword(s):  
Proline Dehydrogenase ◽  
Gene Encoding ◽  
Cis Acting

scholarly journals Salivary (SD-Type) Cystatins: Over One Billion Years in the Making—But to What Purpose?

2002 ◽  
Vol 13 (6) ◽  
pp. 485-508 ◽  
Author(s):  
D.P. Dickinson
Keyword(s):  
Structure Function ◽  
Submandibular Gland ◽  
Cystatin C ◽  
Housekeeping Gene ◽  
Human Saliva ◽  
Protein Family ◽  
The Body ◽  
Developmentally Regulated ◽  
Gene Encoding ◽  
Cysteine Peptidases

Human saliva contains relatively abundant proteins that are related ancestrally in sequence to the cystatin superfamily. Most, although not all, members of this superfamily are potent inhibitors of cysteine peptidases. Four related genes have been identified, CST1, 2, 4 and 5, encoding cystatins SN, SA, S, and D, respectively. CST1, 4, and probably CST5 are now known to be expressed in a limited number of other tissues in the body, primarily in exocrine epithelia, and the term SD-type cystatin is more appropriate than ’salivary cystatin’. These genes are co-ordinately regulated in the submandibular gland during post-natal development. The organization of these tissue-specifically-expressed genes in the genome, and their phylogeny, indicate that they evolved from an ancestral housekeeping gene encoding the ubiquitously expressed cystatin C, and are members of a larger protein family. Their relationship to rat cystatin S, a developmentally regulated rodent submandibular gland protein, remains to be established. In this review, the evolution of the SD-type cystatins in the cystatin superfamily, their genomics, expression, and structure-function relationships are examined and compared with known cystatin functions, with the goal of providing clues to their biological roles.

Pear IAA1 gene encoding an auxin-responsive Aux/IAA protein is involved in fruit development and response to salicylic acid

2014 ◽  
Vol 94 (2) ◽  
pp. 263-271 ◽  
Author(s):  
Haiyan Shi ◽  
Yanhui Wang ◽  
Zhenghong Li ◽  
Diansheng Zhang ◽  
Yufeng Zhang ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Fruit Development ◽  
Phylogenetic Analyses ◽  
Pcr Amplification ◽  
Pyrus Pyrifolia ◽  
Dimerization Domain ◽  
Developmentally Regulated ◽  
Gene Encoding ◽  
Domain Profile ◽  
Localization Signal

Shi, H., Wang, Y., Li, Z., Zhang, D., Zhang, Y., Xiang, D., Li, Y. and Zhang, Y. 2014. Pear IAA1 gene encoding an auxin-responsive Aux/IAA protein is involved in fruit development and response to salicylic acid. Can. J. Plant Sci. 94: 263–271. Auxin-responsive Aux/IAA proteins are rapidly auxin-induced, short-lived proteins that act as repressors for the auxin response factor (ARF)-activated gene expression. A gene encoding an Aux/IAA protein and designated PpIAA1 was isolated from pear (Pyrus pyrifolia). Using PCR amplification techniques, the genomic clone corresponding to PpIAA1 was isolated and shown to contain three introns with typical GT/AG boundaries defining the splice junctions. The deduced PpIAA1 protein contains the conserved features of indole-3-acetic acids (IAA): four Aux/IAA conserved domains, Aux/IAA family domain, Aux/IAA-ARF dimerization domain profile, and conserved nuclear localization signal (NLS) motifs. Phylogenetic analyses clearly demonstrated PpIAA1 has the highest homology with grape VvIAA. PpIAA1 was preferentially expressed in fruit, and moderate expression was found in anthers. Relatively low expression signal was detected in other tissues including shoots, leaves, and petals. Moreover, expression of PpIAA1 was developmentally regulated in fruit. Further study demonstrated that PpIAA1 expression in pear fruit was remarkably regulated by salicylic acid and IAA. The data suggest that PpIAA1 might be involved in the interplay between IAA and salicylic acid signaling pathway during the fruit development of pear.

Evidence for the presence of an NF-kappaB signal transduction system in Dictyostelium discoideum

1999 ◽  
Vol 112 (20) ◽  
pp. 3529-3535 ◽  
Author(s):  
F. Traincard ◽  
E. Ponte ◽  
J. Pun ◽  
B. Coukell ◽  
M. Veron
Keyword(s):  
Signal Transduction ◽  
Transcription Factors ◽  
Dictyostelium Discoideum ◽  
Nuclear Translocation ◽  
Developmental Process ◽  
Signal Transduction System ◽  
Developmentally Regulated ◽  
Gene Encoding ◽  
Nuclear Extracts ◽  
Nf Kappab

The Rel/NF-kappaB family of transcription factors and regulators has so far only been described in vertebrates and arthropods, where they mediate responses to many extracellular signals. No counterparts of genes coding for such proteins have been identified in the Caenorhabditis elegans genome and no NF-kappaB activity was found in Saccharomyces cerevisiae. We describe here the presence of an NF-kappaB transduction pathway in the lower eukaryote Dictyostelium discoideum. Using antibodies raised against components of the mammalian NF-kappaB pathway, we demonstrate in Dictyostelium cells extracts the presence of proteins homologous to Rel/NF-kappaB, IkappaB and IKK components. Using gel-shift experiments in nuclear extracts of developing Dictyostelium cells, we demonstrate the presence of proteins binding to kappaB consensus oligonucleotides and to a GC-rich kappaB-like sequence, lying in the promoter of cbpA, a developmentally regulated Dictyostelium gene encoding the Ca(2+)-binding protein CBP1. Using immunofluorescence, we show specific nuclear translocation of the p65 and p50 homologues of the NF-kappaB transcription factors as vegetatively growing cells develop to the slug stage. Taken together, our results strongly indicate the presence of a complete NF-kappaB signal transduction system in Dictyostelium discoideum that could be involved in the developmental process.

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