scholarly journals Evidence That Auxin-Induced Growth of Tobacco Leaf Tissues Does Not Involve Cell Wall Acidification

1998 ◽  
Vol 118 (2) ◽  
pp. 557-564 ◽  
Author(s):  
Christopher P. Keller ◽  
Elizabeth Van Volkenburgh
Keyword(s):  
Cell Wall ◽  
Tobacco Leaf ◽  
Leaf Tissues ◽  
Cell Wall Acidification

scholarly journals TIR1/AFB-Aux/IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Matyáš Fendrych ◽  
Jeffrey Leung ◽  
Jiří Friml
Keyword(s):  
Cell Wall ◽  
Gene Transcription ◽  
Growth Theory ◽  
Auxin Signaling ◽  
Differential Growth ◽  
Acid Growth ◽  
Genetic Manipulations ◽  
Gravity Response ◽  
Acid Growth Theory ◽  
Cell Wall Acidification

Despite being composed of immobile cells, plants reorient along directional stimuli. The hormone auxin is redistributed in stimulated organs leading to differential growth and bending. Auxin application triggers rapid cell wall acidification and elongation of aerial organs of plants, but the molecular players mediating these effects are still controversial. Here we use genetically-encoded pH and auxin signaling sensors, pharmacological and genetic manipulations available for Arabidopsis etiolated hypocotyls to clarify how auxin is perceived and the downstream growth executed. We show that auxin-induced acidification occurs by local activation of H+-ATPases, which in the context of gravity response is restricted to the lower organ side. This auxin-stimulated acidification and growth require TIR1/AFB-Aux/IAA nuclear auxin perception. In addition, auxin-induced gene transcription and specifically SAUR proteins are crucial downstream mediators of this growth. Our study provides strong experimental support for the acid growth theory and clarified the contribution of the upstream auxin perception mechanisms.

IAA breakdown and its effect on auxin-induced cell wall acidification in maize coleoptile segments

1997 ◽  
Vol 100 (3) ◽  
pp. 415-422 ◽  
Author(s):  
Winfried S. Peters ◽  
Christa Lommel ◽  
Hubert Felle
Keyword(s):  
Cell Wall ◽  
Maize Coleoptile ◽  
Cell Wall Acidification

Detection of urease in the cell wall and membranes from leaf tissues of bromeliad species

2009 ◽  
Vol 136 (1) ◽  
pp. 86-93 ◽  
Author(s):  
Camila Aguetoni Cambuí ◽  
Marí lia Gaspar ◽  
Helenice Mercier
Keyword(s):  
Cell Wall ◽  
Leaf Tissues

scholarly journals TMK-based cell surface auxin signaling activates cell wall acidification in Arabidopsis

2021 ◽  
Author(s):  
Zhenbiao Yang ◽  
Wenwei Lin ◽  
Wenxin Tang ◽  
Koji Takahashi ◽  
Hong Ren ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Cell Surface ◽  
Cell Expansion ◽  
Auxin Signaling ◽  
Molecular Evidence ◽  
Signaling Proteins ◽  
Acid Growth ◽  
Cell Wall Acidification ◽  
Growth Hypothesis

Abstract The phytohormone auxin controls a myriad of processes in plants, at least in part through its regulation of cell expansion. The "acid growth hypothesis" has been proposed to explain auxin-stimulated cell expansion for five decades, but the mechanism underlying auxin-induced cell wall acidification is poorly characterized. Auxin induces the phosphorylation and activation of the plasma membrane (PM) H+-ATPase that pumps protons into the apoplast, yet how auxin activates its phosphorylation remains elusive. Here, we show that the transmembrane kinase (TMK) auxin signaling proteins interact with PM H+-ATPases and activate their phosphorylation to promote cell wall acidification and hypocotyl cell elongation in Arabidopsis. Auxin induced TMK's interaction with H+-ATPase on the plasma membrane within 1-2 minutes as well as TMK-dependent phosphorylation of the penultimate Thr residue. Genetic, biochemical, and molecular evidence demonstrates that TMKs are required for auxin-induced PM H+-ATPase activation, apoplastic acidification, and cell expansion. Thus, our findings reveal a crucial connection between auxin and PM H+-ATPase activation in regulating apoplastic pH changes and cell expansion via TMK-based cell surface auxin signaling.

IAA breakdown and its effect on auxin-induced cell wall acidification in maize coleoptile segments

1997 ◽  
Vol 100 (3) ◽  
pp. 415-422
Author(s):  
Winfried S. Peters ◽  
Christa Lommel ◽  
Hubert Felle
Keyword(s):  
Cell Wall ◽  
Maize Coleoptile ◽  
Cell Wall Acidification

Nature of the guard cell wall in leaf stomata of three Ophioglossum species

1975 ◽  
Vol 53 (16) ◽  
pp. 1698-1711 ◽  
Author(s):  
R. L. Peterson ◽  
M. S. Firminger ◽  
L. A. Dobrindt
Keyword(s):  
Cell Wall ◽  
Guard Cell ◽  
Cell Walls ◽  
Guard Cells ◽  
Aniline Blue ◽  
Phenolic Substance ◽  
Polarization Microscopy ◽  
Leaf Tissues ◽  
Stomatal Guard Cells ◽  
Staining Techniques

A β-1,3-glucan which has characteristics of callose was identified as a component of the cell wall in stomatal guard cells in three species of the fern, Ophioglossum. This identification was made by the fluorochrome properties of callose when stained with aqueous solutions of aniline blue. Controls involved both the effect of solutions of different pH on autofluorescence of guard cell walls and the extraction of leaf tissues with β-1,3-glucanases before staining with aniline blue. An electron-translucent region between the plasmalemma and the cell wall proper was observed with the electron microscope and corresponded in position with the areas that fluoresced after aniline blue staining.Other components of the guard cell wall identified included cellulose, which was identified by staining techniques, polarization microscopy, and electron microscopy; and a phenolic substance identified by a number of staining reactions. The cell wall failed to stain with a number of reagents for the identification of lignin.

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