scholarly journals A Fiberless Seed Mutation in Cotton Is Associated with Lack of Fiber Cell Initiation in Ovule Epidermis and Alterations in Sucrose Synthase Expression and Carbon Partitioning in Developing Seeds

1998 ◽  
Vol 118 (2) ◽  
pp. 399-406 ◽  
Author(s):  
Yong-Ling Ruan ◽  
Prem S. Chourey
Keyword(s):  
Sucrose Synthase ◽  
Carbon Partitioning ◽  
Fiber Cell ◽  
Developing Seeds ◽  
Fiber Cell Initiation

scholarly journals Cytokinin inhibits cotton fiber initiation by disrupting PIN3a-mediated asymmetric accumulation of auxin in the ovule epidermis

2019 ◽  
Vol 70 (12) ◽  
pp. 3139-3151 ◽  
Author(s):  
Jianyan Zeng ◽  
Mi Zhang ◽  
Lei Hou ◽  
Wenqin Bai ◽  
Xingying Yan ◽  
...  
Keyword(s):  
Cotton Fiber ◽  
Plasma Membranes ◽  
Antagonistic Effect ◽  
Fiber Cell ◽  
In Planta ◽  
Polar Localization ◽  
Fiber Cells ◽  
Fiber Initiation ◽  
Fiber Cell Initiation

AbstractAuxin-dependent cell expansion is crucial for initiation of fiber cells in cotton (Gossypium hirsutum), which ultimately determines fiber yield and quality. However, the regulation of this process is far from being well understood. In this study, we demonstrate an antagonistic effect between cytokinin (CK) and auxin on cotton fiber initiation. In vitro and in planta experiments indicate that enhanced CK levels can reduce auxin accumulation in the ovule integument, which may account for the defects in the fiberless mutant xu142fl. In turn, supplementation with auxin can recover fiber growth of CK-treated ovules and mutant ovules. We further found that GhPIN3a is a key auxin transporter for fiber-cell initiation and is polarly localized to the plasma membranes of non-fiber cells, but not to those of fiber cells. This polar localization allows auxin to be transported within the ovule integument while specifically accumulating in fiber cells. We show that CKs antagonize the promotive effect of auxin on fiber cell initiation by undermining asymmetric accumulation of auxin in the ovule epidermis through down-regulation of GhPIN3a and disturbance of the polar localization of the protein.

LCM and RNA-seq analyses revealed roles of cell cycle and translational regulation in early stages of cotton fiber cell development

2020 ◽  
Author(s):  
Atsumi Ando ◽  
Ryan C. Kirkbride ◽  
Don Jones ◽  
Jane Grimwood ◽  
Z. Jeffrey Chen
Keyword(s):  
Cell Cycle ◽  
Transcription Factor ◽  
Cotton Fiber ◽  
Fiber Cell ◽  
Myb Transcription Factor ◽  
Epidermal Layer ◽  
Expression Divergence ◽  
Rna Seq ◽  
Ribosome Biosynthesis ◽  
Fiber Cell Initiation

Abstract BackgroundCotton fibers provide a powerful model for studying cell differentiation and elongation. Each cotton fiber is a singular and elongated cell derived from epidermal-layer cells of a cotton seed. Efforts to understand this dramatic developmental shift have been impeded by the difficulty of isolating fiber cells from epidermal cells.ResultsHere we employed laser-capture microdissection (LCM) to separate these cell types. RNA-seq analysis revealed transitional differences between the fiber and epidermal-layer cells at 0 or 2 days post anthesis. Specifically, down-regulation of putative cell cycle genes was coupled with upregulation of ribosome biosynthesis and translation-related genes, which may suggest their respective roles in fiber cell initiation and elongation. Indeed, the amount of fibers in cultured ovules was increased by cell cycle progression inhibitor, Roscovitine, and decreased by ribosome biosynthesis inhibitor, Rbin-1. Moreover, many phytohormone-related genes were upregulated in the ovules and down-regulated in the fibers, suggesting their spatial-temporal effects on fiber cell development. Key cell cycle regulators were predicted to be epialleles, and MYB-transcription factor related genes displayed expression divergence between fibers and ovules, implying their effects on fiber traits.ConclusionsWe revealed that fiber cell initiation is accompanied by cell cycle arrest coupled with active ribosome biosynthesis, spatial-temporal regulation of phytohormones and expression divergence between MYB transcription factor genes. These valuable genomic resources and molecular insights will help develop breeding and biotechnological tools to improve cotton fiber production.

scholarly journals GhDET2, a steroid 5α-reductase, plays an important role in cotton fiber cell initiation and elongation

2007 ◽  
Vol 51 (3) ◽  
pp. 419-430 ◽  
Author(s):  
Ming Luo ◽  
Yuehua Xiao ◽  
Xianbi Li ◽  
Xiaofeng Lu ◽  
Wei Deng ◽  
...  
Keyword(s):  
Cotton Fiber ◽  
Fiber Cell ◽  
Fiber Cell Initiation
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