scholarly journals Cutin Monomers and Surface Wax Constituents Elicit H2O2 in Conditioned Cucumber Hypocotyl Segments and Enhance the Activity of Other H2O2Elicitors

1998 ◽  
Vol 117 (4) ◽  
pp. 1373-1380 ◽  
Author(s):  
Markus Fauth ◽  
Patrick Schweizer ◽  
Antony Buchala ◽  
Claus Markstädter ◽  
Markus Riederer ◽  
...  
Keyword(s):  
Surface Wax ◽  
Cucumber Hypocotyl ◽  
Hypocotyl Segments

ACTIONS AND INTERACTIONS OF GROWTH FACTORS ON CUCUMBER HYPOCOTYL SEGMENTS

1967 ◽  
Vol 144 (1 Plant Growth) ◽  
pp. 169-179 ◽  
Author(s):  
William K. Purves ◽  
David L. Rayle ◽  
Kenneth D. Johnson
Keyword(s):  
Growth Factors ◽  
Cucumber Hypocotyl ◽  
Hypocotyl Segments

The Role of the Cotyledons in Gibberellin- and Auxin-Induced Elongation of the Cucumber Hypocotyl

1965 ◽  
Vol 18 (2) ◽  
pp. 550-556 ◽  
Author(s):  
Masayuki Katsumi ◽  
William K. Purves ◽  
Bernard O. Phinney ◽  
Jiro Kato
Keyword(s):  
Cucumber Hypocotyl

Agrobacterium tumefaciens-mediated Transformation of Double Haploid Canola (Brassica napus) Lines

1997 ◽  
Vol 24 (1) ◽  
pp. 97 ◽  
Author(s):  
K. Kazan ◽  
M. D. Curtis ◽  
K. C. Goulter ◽  
J. M. Manners
Keyword(s):  
Brassica Napus ◽  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Genetic Transformation ◽  
Double Haploid ◽  
Gene Promoter ◽  
Root Explants ◽  
Hypocotyl Explants ◽  
Peroxidase Gene ◽  
Hypocotyl Segments

Double haploid (DH) genotypes of canola (Brassica napus L.) have a high level of genetic uniformity but have not been previously tested for genetic transformation. Transgenic plants from three of four DH genotypes derived from cv. Westar were obtained by inoculation of either hypocotyl segments or root explants with Agrobacterium tumefaciens. For hypocotyl transformation, A. tumefaciens strain LBA4404 containing a binary plasmid with the neomycin phosphotransferase gene (nptII) and a CaMV 35S-peroxidase gene cassette was co-cultivated with hypocotyl segments taken from the 5–6-day-old seedlings. Transformation frequencies for hypocotyl explants of two DH genotypes were 0.3–3%. Direct evidence for genetic transformation of hypocotyl explants was obtained through molecular hybridisation analysis. Using this protocol, mature transformed plants were obtained within 4–6 months of co-cultivation. A method of root transformation was successfully modified for one DH genotype of canola and transgenic plants were obtained at a frequency of 2%. Using this protocol, a peroxidase gene promoter–GUS fusion construct was introduced into a DH genotype. Tissue specific GUS expression driven by the peroxidase gene promoter in transgenic plants was analysed by GUS staining. Transformation systems for double haploid canola lines will permit the assessment of introduced genes for their effect on agronomic and physiological traits.

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