scholarly journals Mutagenesis of the Glucose-1-Phosphate-Binding Site of Potato Tuber ADP-Glucose Pyrophosphorylase

1998 ◽  
Vol 117 (3) ◽  
pp. 989-996 ◽  
Author(s):  
Yingbin Fu ◽  
Miguel A. Ballicora ◽  
Jack Preiss
Keyword(s):  
Potato Tuber ◽  
Binding Site ◽  
Phosphate Binding ◽  
Adp Glucose Pyrophosphorylase

Phosphate Binding to Isolated Chloroplast Coupling Factor (CF1)

1988 ◽  
Vol 43 (3-4) ◽  
pp. 213-218 ◽  
Author(s):  
Bernhard Huchzermeyer
Keyword(s):  
Atpase Activity ◽  
Binding Site ◽  
Inorganic Phosphate ◽  
Coupling Factor ◽  
Binding Domain ◽  
Atp Binding ◽  
Phosphate Binding ◽  
Chloroplast Coupling Factor ◽  
Single Binding Site ◽  
Site Binding

A single binding site for phosphate was found on isolated chloroplast coupling factor in the absence of nucleotides. In our experiments the phosphate binding site showed a Kd of 170 μᴍ. We did not observe any differences whether the ATPase activity of CF] had been activated or not. If the enzyme was incubated with [γ-32P]ATP the amount of 32P bound per CF1 depended on the pretreatment of the enzyme: In the presence of ADP no ATP or phosphate was bound to CF,. After activation of ATPase activity one mol of ATP per mol CF, was rapidly bound and hydrolyzed while there was a slowly occurring binding of another phosphate without concomitant nucleotide binding. We conclude that there are two different types of phosphate binding observed in our experiments: 1) Inorganic phosphate can be bound by one catalytic site per mol of CF1 2) The γ-phosphate of ATP is able to bind to an ATP binding domain of the enzyme if this domain can exchange substrates with the incubation medium. This ATP binding domain appears to differ from the site binding inorganic phosphate, because at least a portion of the coupling factor contains more than one labelled phosphate during our ATPase tests.

scholarly journals Identification of a fourth mannose 6-phosphate binding site in the cation-independent mannose 6-phosphate receptor

Glycobiology ◽  
2015 ◽  
Vol 25 (6) ◽  
pp. 591-606 ◽  
Author(s):  
Linda J Olson ◽  
Alicia C Castonguay ◽  
Yi Lasanajak ◽  
Francis C Peterson ◽  
Richard D Cummings ◽  
...  
Keyword(s):  
Binding Site ◽  
Phosphate Binding ◽  
Mannose 6 Phosphate

Characterization of an RNase with two catalytic centers. Human RNase6 catalytic and phosphate-binding site arrangement favors the endonuclease cleavage of polymeric substrates

2019 ◽  
Vol 1863 (1) ◽  
pp. 105-117 ◽  
Author(s):  
Guillem Prats-Ejarque ◽  
Jose A. Blanco ◽  
Vivian A. Salazar ◽  
Victòria M. Nogués ◽  
Mohammed Moussaoui ◽  
...  
Keyword(s):  
Binding Site ◽  
Phosphate Binding ◽  
Polymeric Substrates

scholarly journals Comparison of Saccharomyces cerevisiae F-BAR Domain Structures Reveals a Conserved Inositol Phosphate Binding Site

Structure ◽  
2015 ◽  
Vol 23 (2) ◽  
pp. 352-363 ◽  
Author(s):  
Katarina Moravcevic ◽  
Diego Alvarado ◽  
Karl R. Schmitz ◽  
Jon A. Kenniston ◽  
Jeannine M. Mendrola ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Binding Site ◽  
Inositol Phosphate ◽  
Phosphate Binding ◽  
Bar Domain ◽  
Domain Structures
Sign in / Sign up

Export Citation Format

Share Document