scholarly journals Influence of Precursor Availability on Alkaloid Accumulation by Transgenic Cell Line of Catharanthus roseus

1998 ◽  
Vol 116 (2) ◽  
pp. 853-857 ◽  
Author(s):  
Serap Whitmer ◽  
Camilo Canel ◽  
Didier Hallard ◽  
Cecilia Gonçalves ◽  
Robert Verpoorte
Keyword(s):  
Cell Line ◽  
Catharanthus Roseus ◽  
Transgenic Cell ◽  
Transgenic Cell Line

22 PRODUCTION OF PORCINE TRANSGENIC CELL LINE INSERTED WITH SV40LT, EGFRvIII Gene, AND INDUCIBLE CreERT SYSTEM

2016 ◽  
Vol 28 (2) ◽  
pp. 141
Author(s):  
S. U. Hwang ◽  
J. D. Yoon ◽  
K. Y. Eun ◽  
H. G. Kim ◽  
S. H. Hyun
Keyword(s):  
Cell Line ◽  
Embryo Development ◽  
Donor Cell ◽  
Disease Models ◽  
Blastocyst Formation ◽  
Cooperative Research ◽  
Normal Cells ◽  
Transgenic Cell ◽  
Significant Difference ◽  
Transgenic Cell Line

Transgenic pigs are currently believed to be an important model for biomedical research, including for disease models, pharmaceutical toxicity testing, and regenerative medicine. However, production efficiency of animal disease models using somatic cell NT (SCNT) is very low. One of the main reasons is probably characteristics of the transgene. In this study, we introduce SV40LT oncogene into the fibroblast cells in order to establish immortalized transgenic cell line for producing the pig model of human brain cancer. We evaluated the effect of SV40LT oncogene on transgenic SCNT embryo development. As a results, the cleavage rates (73.8 ± 4.0 and 48.6 ± 2.4 in the normal and SV40LT group, respectively; P < 0.05) and blastocyst formation rates (19.5 ± 1.2 and 5.6 ± 1.8 in the normal and SV40LT group, respectively; P < 0.05) of transgenic SCNT embryos was significantly lower than the case of using normal cells. In addition, we evaluated the transgenic SCNT embryo development of the donor cell transfected with SV40LT and HrasV12 genes (SV40LT+HrasV12 group). As a results, there was no significant difference between the groups in the cleavage rates, but blastocyst formation rates of transfected SCNT embryos (SV40LT+HrasV12 group) was significantly lower than the case of using normal cells (19.5 ± 1.2 and 6.2 ± 1.8 in the normal and SV40LT+HrasV12 group, respectively; P < 0.05). Genes SV40LT or HrasV12 showed a negative effect on SCNT cloned embryo development. Therefore, a Cre/loxP inducible system was applied to producing donor cells transfected with EGFRvIII and SV40LT gene. As a result, the cleavage rates (73.8 ± 4.0 and 68.6 ± 6.6 in the normal and Cre/loxP-EGFRvIII-SV40LT group, respectively; P < 0.05) and blastocyst formation rate (19.5 ± 1.2 and 23.0 ± 3.7 in the normal and Cre/loxP-EGFRvIII-SV40LT group, respectively; P < 0.05) were improved to the same level, when used as a donor cell to a normal cell. In conclusion, these results indicated that harmful effects of transgenic SCNT embryo development caused by the characteristics of the inserted genes can be overcome through the inducible system. Further studies are needed to experiment with mRNA expression of apoptotic gene and target gene in 4- to 8-cell embryos and blastocysts. This work was supported, in part, by a grant from the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ011077, PJ011288), Rural Development Administration, and the National Research Foundation of Korea Grant funded by the Korean government (NRF-2013R1A2A2A04008751), Republic of Korea.

scholarly journals Developing a Triple Transgenic Cell Line for High-Efficiency Porcine Reproductive and Respiratory Syndrome Virus Infection

PLoS ONE ◽  
2016 ◽  
Vol 11 (5) ◽  
pp. e0154238 ◽  
Author(s):  
Linlin Zhang ◽  
Zhengzhi Cui ◽  
Lei Zhou ◽  
Youmin Kang ◽  
Li Li ◽  
...  
Keyword(s):  
Virus Infection ◽  
Cell Line ◽  
High Efficiency ◽  
Respiratory Syndrome Virus ◽  
Transgenic Cell ◽  
Transgenic Cell Line

scholarly journals Correction: Developing a Triple Transgenic Cell Line for High-Efficiency Porcine Reproductive and Respiratory Syndrome Virus Infection

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0160325 ◽  
Author(s):  
Linlin Zhang ◽  
Zhengzhi Cui ◽  
Lei Zhou ◽  
Youmin Kang ◽  
Li Li ◽  
...  
Keyword(s):  
Virus Infection ◽  
Cell Line ◽  
High Efficiency ◽  
Respiratory Syndrome Virus ◽  
Transgenic Cell ◽  
Transgenic Cell Line

scholarly journals Potential limitations of the Sleeping Beauty transposon use in gene expression studies

2019 ◽  
Author(s):  
Weronika Sowińska ◽  
Mateusz Wawro ◽  
Aleksandra Solecka ◽  
Aneta Kasza
Keyword(s):  
Transcript Level ◽  
Sleeping Beauty ◽  
Full Spectrum ◽  
Cellular Targets ◽  
Sleeping Beauty Transposon ◽  
Transgenic Cell ◽  
Expression Studies ◽  
Rapid Generation ◽  
Gene Expression Studies ◽  
Transgenic Cell Line

MCPIP2 is the least known member of the MCPIP family of proteins. Recently we have found that it is a new RNase involved in transcript turnover. However, the full spectrum of its cellular targets is still unidentified. To discover transcripts which are regulated by this protein we have employed Sleeping Beauty transposons. This tool allows for rapid generation of a stable transgenic cell line with inducible expression of the desired gene. In this study, we analysed how the Sleeping Beauty system itself influences expression of chosen genes, namely IL-6, Regnase-1 and VEGF. We found that the system alone may influence expression of IL-6. Our results indicate that Sleeping Beauty transposons should be used with caution in studies that are focused on changes in the transcript level.

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