scholarly journals Seed Germination of Arabidopsis thaliana phyA/phyB Double Mutants Is under Phytochrome Control

1997 ◽  
Vol 114 (4) ◽  
pp. 1487-1492 ◽  
Author(s):  
C. Poppe ◽  
E. Schafer
Keyword(s):  
Arabidopsis Thaliana ◽  
Seed Germination

Ontogenetic, Light, and Circadian Regulation of PAP Protein Genes during Seed Germination of Arabidopsis thaliana

2021 ◽  
Vol 500 (1) ◽  
pp. 312-316
Author(s):  
A. A. Andreeva ◽  
N. V. Kudryakova ◽  
Vl. V. Kuznetsov ◽  
V. V. Kusnetsov
Keyword(s):  
Arabidopsis Thaliana ◽  
Seed Germination ◽  
Circadian Regulation

scholarly journals Genome-Wide Characterization and Expression Analysis of KH Family Genes Response to ABA and SA in Arabidopsis thaliana

2022 ◽  
Vol 23 (1) ◽  
pp. 511
Author(s):  
Yanjie Zhang ◽  
Yu Ma ◽  
Ruiqi Liu ◽  
Guanglin Li
Keyword(s):  
Arabidopsis Thaliana ◽  
Seed Germination ◽  
Nucleic Acid Binding ◽  
Phylogenetic Tree Analysis ◽  
Genome Wide ◽  
Binding Factor ◽  
Pathogenesis Related ◽  
Kh Domain ◽  
Family Based ◽  
Related Proteins

K-homologous (KH) family is a type of nucleic acid-binding protein containing the KH domain and has been found to affect splicing and transcriptional regulation. However, KH family genes haven’t been investigated in plant species systematically. In this study, we identified 30 genes that belonged to the KH family based on HMM of the KH domain in Arabidopsis thaliana. Phylogenetic tree analysis showed that the KH family is grouped into three subgroups. Synteny analysis showed that AtKH9 and AtKH29 have the conserved synteny relationship between A. thaliana and the other five species. The AtKH9 and AtKH29 were located in the cytoplasm and nucleus. The seed germination rates of the mutants atkh9 and atkh29 were higher than wild-type after abscisic acid (ABA) and salicylic acid (SA) treatments. In addition, the expression of ABA-related genes, such as ABRE-binding factor 2 (ABF2), ABRE-binding factor 4 (ABF4), and delta 1-pyrroline-5-carboxylate synthase (P5CS), and an SA-related gene pathogenesis-related proteins b (PR1b) were downregulated after ABA and SA treatments, respectively. These results suggested that atkh9 and atkh29 mutants inhibit the effect of ABA and SA on seed germination. In conclusion, our results provide valuable information for further exploration of the function of KH family genes and propose directions and ideas for the identification and characterization of KH family genes in other plants.

An RRM-containing mei2 -like MCT1 plays a negative role in the seed germination and seedling growth of Arabidopsis thaliana in the presence of ABA

2016 ◽  
Vol 109 ◽  
pp. 273-279 ◽  
Author(s):  
Lili Gu ◽  
Hyun Ju Jung ◽  
Kyung Jin Kwak ◽  
Sy Nguyen Dinh ◽  
Yeon-Ok Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Seed Germination ◽  
Seedling Growth ◽  
Negative Role

DREB2 Family Transcription Factors Promote the Expression of Phytocystatin1 in Arabidopsis thaliana During Seed Germination

2020 ◽  
Author(s):  
Taeyoon Kim ◽  
Trang Thi Nguyen ◽  
Juwan Baek ◽  
Young Hun Song ◽  
Jong Chan Hong ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Seed Germination

scholarly journals Arabidopsis chloroplast J protein DJC75/CRRJ mediates nitrate-promoted seed germination in the dark

2020 ◽  
Vol 125 (7) ◽  
pp. 1091-1099
Author(s):  
Huai-Syuan Ciou ◽  
Yu-Lun Tsai ◽  
Chi-Chou Chiu
Keyword(s):  
Arabidopsis Thaliana ◽  
Seed Germination ◽  
Germination Rate ◽  
Red Light ◽  
Phytochrome B ◽  
Biosynthetic Gene ◽  
Unknown Mechanism ◽  
J Domain ◽  
Domain Protein ◽  
J Protein

Abstract Background and Aims Nitrate can stimulate seed germination of many plant species in the absence of light; however, the molecular mechanism of nitrate-promoted seed germination in the dark remains largely unclear and no component of this pathway has been identified yet. Here, we show that a plastid J-domain protein, DJC75/CRRJ, in arabidopsis (Arabidopsis thaliana) is important for nitrate-promoted seed germination in the dark. Methods The expression of DJC75 during imbibition in the dark was investigated. The seed germination rate of mutants defective in DJC75 was determined in the presence of nitrate when light cues for seed germination were eliminated by the treatment of imbibed seeds with a pulse of far-red light to inactivate phytochrome B (phyB), or by assaying germination in the dark with seeds harbouring the phyB mutation. The germination rates of mutants defective in CRRL, a J-like protein related to DJC75, and in two chloroplast Hsp70s were also measured in the presence of nitrate in darkness. Key Results DJC75 was expressed during seed imbibition in the absence of light. Mutants defective in DJC75 showed seed germination defects in the presence of nitrate when light cues for seed germination were eliminated. Mutants defective in CRRL and in two chloroplast Hsp70s also exhibited similar seed germination defects. Upregulation of gibberellin biosynthetic gene GA3ox1 expression by nitrate in imbibed phyB mutant seeds was diminished when DJC75 was knocked out. Conclusions Our data suggest that plastid J-domain protein DJC75 regulates nitrate-promoted seed germination in the dark by upregulation of expression of the gibberellin biosynthetic gene GA3ox1 through an unknown mechanism and that DJC75 may work in concert with chloroplast Hsp70s to regulate nitrate-promoted seed germination. DJC75 is the first pathway component identified for nitrate-promoted seed germination in the dark.

scholarly journals LPIAT, a lyso-Phosphatidylinositol Acyltransferase, Modulates Seed Germination in Arabidopsis thaliana through PIP Signalling Pathways and is Involved in Hyperosmotic Response

2020 ◽  
Vol 21 (5) ◽  
pp. 1654
Author(s):  
Denis Coulon ◽  
Lionel Faure ◽  
Magali Grison ◽  
Stéphanie Pascal ◽  
Valérie Wattelet-Boyer ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Seed Germination ◽  
Lipid Synthesis ◽  
Strong Increase ◽  
In Planta ◽  
Acyltransferase Activity ◽  
Chain Acyl ◽  
Phosphatidylinositol Phosphates ◽  
Mature Seeds

Lyso-lipid acyltransferases are enzymes involved in various processes such as lipid synthesis and remodelling. Here, we characterized the activity of an acyltransferase from Arabidopsis thaliana (LPIAT). In vitro, this protein, expressed in Escherichia coli membrane, displayed a 2-lyso-phosphatidylinositol acyltransferase activity with a specificity towards saturated long chain acyl CoAs (C16:0- and C18:0-CoAs), allowing the remodelling of phosphatidylinositol. In planta, LPIAT gene was expressed in mature seeds and very transiently during seed imbibition, mostly in aleurone-like layer cells. Whereas the disruption of this gene did not alter the lipid composition of seed, its overexpression in leaves promoted a strong increase in the phosphatidylinositol phosphates (PIP) level without affecting the PIP2 content. The spatial and temporal narrow expression of this gene as well as the modification of PIP metabolism led us to investigate its role in the control of seed germination. Seeds from the lpiat mutant germinated faster and were less sensitive to abscisic acid (ABA) than wild-type or overexpressing lines. We also showed that the protective effect of ABA on young seedlings against dryness was reduced for lpiat line. In addition, germination of lpiat mutant seeds was more sensitive to hyperosmotic stress. All these results suggest a link between phosphoinositides and ABA signalling in the control of seed germination

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