scholarly journals Rapid Identification of a Natural Knockout Allele of ARMADILLO REPEAT-CONTAINING KINESIN1 That Causes Root Hair Branching by Mapping-By-Sequencing

2014 ◽  
Vol 166 (3) ◽  
pp. 1280-1287 ◽  
Author(s):  
L. Rishmawi ◽  
H. Sun ◽  
K. Schneeberger ◽  
M. Hulskamp ◽  
A. Schrader
Keyword(s):  
Root Hair ◽  
Rapid Identification ◽  
Armadillo Repeat ◽  
Mapping By Sequencing ◽  
Knockout Allele

scholarly journals Easymap: A User-Friendly Software Package for Rapid Mapping-by-Sequencing of Point Mutations and Large Insertions

2021 ◽  
Vol 12 ◽  
Author(s):  
Samuel Daniel Lup ◽  
David Wilson-Sánchez ◽  
Sergio Andreu-Sánchez ◽  
José Luis Micol
Keyword(s):  
Point Mutations ◽  
Rapid Identification ◽  
Graphical Interface ◽  
Rna Seq ◽  
Source Program ◽  
Next Generation Sequencing Ngs ◽  
User Friendly ◽  
Ngs Data ◽  
Mapping By Sequencing ◽  
Generation Sequencing

Mapping-by-sequencing strategies combine next-generation sequencing (NGS) with classical linkage analysis, allowing rapid identification of the causal mutations of the phenotypes exhibited by mutants isolated in a genetic screen. Computer programs that analyze NGS data obtained from a mapping population of individuals derived from a mutant of interest to identify a causal mutation are available; however, the installation and usage of such programs requires bioinformatic skills, modifying or combining pieces of existing software, or purchasing licenses. To ease this process, we developed Easymap, an open-source program that simplifies the data analysis workflows from raw NGS reads to candidate mutations. Easymap can perform bulked segregant mapping of point mutations induced by ethyl methanesulfonate (EMS) with DNA-seq or RNA-seq datasets, as well as tagged-sequence mapping for large insertions, such as transposons or T-DNAs. The mapping analyses implemented in Easymap have been validated with experimental and simulated datasets from different plant and animal model species. Easymap was designed to be accessible to all users regardless of their bioinformatics skills by implementing a user-friendly graphical interface, a simple universal installation script, and detailed mapping reports, including informative images and complementary data for assessment of the mapping results. Easymap is available at http://genetics.edu.umh.es/resources/easymap; its Quickstart Installation Guide details the recommended procedure for installation.

Rapid identification of causal mutations in tomato EMS populations via mapping-by-sequencing

2016 ◽  
Vol 11 (12) ◽  
pp. 2401-2418 ◽  
Author(s):  
Virginie Garcia ◽  
Cécile Bres ◽  
Daniel Just ◽  
Lucie Fernandez ◽  
Fabienne Wong Jun Tai ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Mapping By Sequencing

scholarly journals Armadillo-repeat kinesin1 interacts with Arabidopsis atlastin RHD3 to move ER with plus-end of microtubules

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiaqi Sun ◽  
Mi Zhang ◽  
Xingyun Qi ◽  
Caitlin Doyle ◽  
Huanquan Zheng
Keyword(s):  
Endoplasmic Reticulum ◽  
Hair Cells ◽  
Root Hair ◽  
Tip Growth ◽  
Living Cells ◽  
Genetic Screen ◽  
Mutant Phenotype ◽  
Armadillo Repeat ◽  
Root Hair Cells ◽  
Subapical Region

Abstract In living cells, dynamics of the endoplasmic reticulum (ER) are driven by the cytoskeleton motor machinery as well as the action of ER-shaping proteins such as atlastin GTPases including RHD3 in Arabidopsis. It is not known if the two systems interplay, and, if so, how they do. Here we report the identification of ARK1 (Armadillo-Repeat Kinesin1) via a genetic screen for enhancers of the rhd3 mutant phenotype. In addition to defects in microtubule dynamics, ER organization is also defective in mutants lacking a functional ARK1. In growing root hair cells, ARK1 comets predominantly localize on the growing-end of microtubules and partially overlap with RHD3 in the cortex of the subapical region. ARK1 co-moves with RHD3 during tip growth of root hair cells. We show that there is a functional interdependence between ARK1 and RHD3. ARK1 physically interacts with RHD3 via its armadillo domain (ARM). In leaf epidermal cells where a polygonal ER network can be resolved, ARK1, but not ARK1ΔARM, moves together with RHD3 to pull an ER tubule toward another and stays with the newly formed 3-way junction of the ER for a while. We conclude that ARK1 acts together with RHD3 to move the ER on microtubules to generate a fine ER network.

scholarly journals Easymap: a user-friendly software package for rapid mapping by sequencing of point mutations and large insertions

2021 ◽  
Author(s):  
Samuel Daniel Lup ◽  
David Wilson-Sánchez ◽  
Sergio Andreu-Sánchez ◽  
José Luis Micol
Keyword(s):  
Point Mutations ◽  
Rapid Identification ◽  
Rna Seq ◽  
Source Program ◽  
Ethylmethane Sulfonate ◽  
Next Generation Sequencing Ngs ◽  
User Friendly ◽  
Ngs Data ◽  
Mapping By Sequencing ◽  
Generation Sequencing

Mapping-by-sequencing strategies combine next-generation sequencing (NGS) with classical linkage analysis, allowing rapid identification of the causal mutations of the phenotypes exhibited by mutants isolated in a genetic screen. Computer programs that analyze NGS data obtained from a mapping population of individuals derived from a mutant of interest in order to identify a causal mutation are available; however, the installation and usage of such programs requires bioinformatic skills, modifying or combining pieces of existent software, or purchasing licenses. To ease this process, we developed Easymap, an open-source program that simplifies the data analysis workflows from raw NGS reads to candidate mutations. Easymap can perform bulked segregant mapping of point mutations induced by ethylmethane sulfonate (EMS) with DNA-seq or RNA-seq datasets, as well as tagged-sequence mapping for large insertions, such as transposons or T-DNAs. The mapping analyses implemented in Easymap have been validated with experimental and simulated datasets from different plant and animal model species. Easymap was designed to be accessible to all users regardless of their bioinformatics skills by implementing a user-friendly graphical interface, a simple universal installation script, and detailed mapping reports, including informative images and complementary data for assessment of the mapping results.

SEM and fluorescence imaging of callose deposition in root hair tips and suspension cultures of Streptanthus tortuosus

1990 ◽  
Vol 48 (3) ◽  
pp. 698-699
Author(s):  
K.S. Walters ◽  
R.D. Sjolund ◽  
K.C. Moore
Keyword(s):  
Cell Wall ◽  
Root Hair ◽  
Cultured Cells ◽  
Root Hairs ◽  
Callus Cultures ◽  
Callose Deposition ◽  
Culture Cells ◽  
Wall Structures ◽  
Ultraviolet Illumination ◽  
Callose Formation

Callose, B-1,3-glucan, a component of cell walls, is associated with phloem sieve plates, plasmodesmata, and other cell wall structures that are formed in response to wounding or infection. Callose reacts with aniline blue to form a fluorescent complex that can be recognized in the light microscope with ultraviolet illumination. We have identified callose in cell wall protuberances that are formed spontaneously in suspension-cultured cells of S. tortuosus and in the tips of root hairs formed in sterile callus cultures of S. tortuosus. Callose deposits in root hairs are restricted to root hair tips which appear to be damaged or deformed, while normal root hair tips lack callose deposits. The callose deposits found in suspension culture cells are restricted to regions where unusual outgrowths or protuberances are formed on the cell surfaces, specifically regions that are the sites of new cell wall formation.Callose formation has been shown to be regulated by intracellular calcium levels.

Endovascular management of ruptured abdominal aortic aneurysms and acute aortic dissections

VASA ◽  
2019 ◽  
Vol 48 (1) ◽  
pp. 35-46
Author(s):  
Stephen Hofmeister ◽  
Matthew B. Thomas ◽  
Joseph Paulisin ◽  
Nicolas J. Mouawad
Keyword(s):  
Abdominal Aortic Aneurysms ◽  
Rapid Identification ◽  
Aortic Aneurysms ◽  
Endovascular Interventions ◽  
Current State ◽  
Technological Advances ◽  
Abdominal Aortic ◽  
Ruptured Abdominal Aortic Aneurysms ◽  
Vascular Emergencies ◽  
Aortic Dissections

Abstract. The management of vascular emergencies is dependent on rapid identification and confirmation of the diagnosis with concurrent patient stabilization prior to immediate transfer to the operating suite. A variety of technological advances in diagnostic imaging as well as the advent of minimally invasive endovascular interventions have shifted the contemporary treatment algorithms of such pathologies. This review provides a comprehensive discussion on the current state and future trends in the management of ruptured abdominal aortic aneurysms as well as acute aortic dissections.

Cullin 3 is a partner of Armadillo Repeat Containing 5 (ARMC5), the product of the gene responsible for Primary Bilateral Macronodular Adrenal Hyperplasia

2018 ◽  
Author(s):  
Isadora Cavalcante ◽  
Eric Clauser ◽  
Anna Vaczlavik ◽  
Ludivine Drougat ◽  
Claudimara Lotfi ◽  
...  
Keyword(s):  
Adrenal Hyperplasia ◽  
Cullin 3 ◽  
Armadillo Repeat
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