scholarly journals Distinct Copy Number, Coding Sequence, and Locus Methylation Patterns Underlie Rhg1-Mediated Soybean Resistance to Soybean Cyst Nematode

2014 ◽  
Vol 165 (2) ◽  
pp. 630-647 ◽  
Author(s):  
David E. Cook ◽  
Adam M. Bayless ◽  
Kai Wang ◽  
Xiaoli Guo ◽  
Qijian Song ◽  
...  
Keyword(s):  
Copy Number ◽  
Soybean Cyst Nematode ◽  
Cyst Nematode ◽  
Coding Sequence ◽  
Soybean Resistance ◽  
Methylation Patterns

scholarly journals The Soybean Resistance Gene Rag1 Does Not Protect Against Soybean Cyst and Root-knot Nematodes

2009 ◽  
Vol 10 (1) ◽  
pp. 44
Author(s):  
Matthew Studham ◽  
Gustavo C. MacIntosh ◽  
Felicitas Avendaño ◽  
David Soh ◽  
Gregory L. Tylka
Keyword(s):  
Resistance Gene ◽  
Soybean Cyst Nematode ◽  
Soybean Aphid ◽  
Cyst Nematode ◽  
Root Knot Nematodes ◽  
Rag1 Gene ◽  
Tomato Gene ◽  
Soybean Resistance

The only well-characterized gene controlling soybean aphid (SBA) resistance is Mi-1.2, a tomato gene that also confers resistance to root-knot nematodes (RKN). Based on similarities between Mi-1.2 and Rag1, which produces a strong antibiosis-type resistance, the authors hypothesized that Rag1 could also provide resistance to nematodes. They evaluated two soybean lines, one carrying the Rag1 gene, and one with no resistance gene, for susceptibility or resistance to soybean cyst nematode (SCN) and RKN. Accepted for publication 4 March 2009. Published 1 April 2009.

scholarly journals Host-Derived Artificial MicroRNA as an Alternative Method to Improve Soybean Resistance to Soybean Cyst Nematode

Genes ◽  
2016 ◽  
Vol 7 (12) ◽  
pp. 122 ◽  
Author(s):  
Bin Tian ◽  
Jiarui Li ◽  
Thomas Oakley ◽  
Timothy Todd ◽  
Harold Trick
Keyword(s):  
Soybean Cyst Nematode ◽  
Cyst Nematode ◽  
Artificial Microrna ◽  
Alternative Method ◽  
Soybean Resistance

Identification of the full-length Hs1pro-1 coding sequence and preliminary evaluation of soybean cyst nematode resistance in soybean transformed with Hs1pro-1 cDNA

2007 ◽  
Vol 85 (4) ◽  
pp. 437-441 ◽  
Author(s):  
Michael D. McLean ◽  
Gordon J. Hoover ◽  
Bonnie Bancroft ◽  
Amina Makhmoudova ◽  
Shawn M. Clark ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Soybean Cyst Nematode ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Full Length ◽  
Preliminary Evaluation ◽  
Chain Reaction ◽  
Coding Sequence ◽  
Scn Resistance ◽  
Polymerase Chain

The Hs1pro-1 gene reportedly confers resistance to the beet cyst nematode in wild beet and sugar beet. Here, we tested the hypothesis that Hs1pro-1 confers resistance in soybean against the soybean cyst nematode (SCN). The full-length Hs1pro-1 coding sequence, which encodes a predicted polypeptide of 490 amino acids, was first acquired then expressed in ‘Westag’ soybean using a constitutive octopine synthase – mannopine synthase promoter. Thirty T0 lines that successfully expressed the Hs1pro-1 gene, as indicated by both polymerase chain reaction and reverse transcriptase – polymerase chain reaction analyses, were generated. Bioassay of the T1 progeny from these lines revealed that only five T0 lines grew normally and exhibited a high degree of SCN resistance. On average, these T1 transgenic progeny were about 70% more resistant to SCN than susceptible control cultivars. These preliminary data suggest that Hs1pro-1 is a promising candidate for genetically engineering SCN resistance in elite, locally adapted soybean cultivars.

scholarly journals Copy Number Quantification for the Soybean Cyst Nematode Resistance Locus rhg1 in the Soybean Varieties of Taiwan

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1346
Author(s):  
Cheng-Chun Huang ◽  
Jiue-in Yang ◽  
Kuo-Lung Chou ◽  
Chen-Hsiang Lin ◽  
Hao-Xun Chang
Keyword(s):  
Copy Number ◽  
Soybean Cyst Nematode ◽  
Crop Protection ◽  
Nematode Resistance ◽  
The United States ◽  
Cyst Nematode ◽  
Agricultural Product ◽  
Resistance Locus ◽  
Nucleotide Polymorphisms ◽  
Market Needs

Disease resistance is one of the most successful strategies in crop protection. For example, the implementation of PI 88788 type resistance, which contains high copy numbers of Resistance to Heterodera glycines 1 (rhg1) loci, into the commercial soybean varieties of the United States has significantly reduced the yield losses caused by soybean cyst nematode (SCN, H. glycines). Vegetable soybean, or edamame, has become a major exporting agricultural product in Taiwan with an annual revenue over $80 million USD since 2017. Several local varieties have been developed to fulfill the market needs such as the traits of flavor and sweetness. However, it remains unclear if the historical breeding programs ever incorporated rhg1 resistance into the varieties of Taiwan. This study applied the TaqMan qPCR method to measure the fluorescent signals specific to the rhg1 locus on the chromosome 18 of soybean, and the ratio of VIC and FAM signals were analyzed to predict the rhg1 copy number in the 21 soybean varieties of Taiwan. The results indicated the copy number and the single nucleotide polymorphisms of the 21 soybean varieties were identical to the susceptible soybean variety ‘Williams 82’. As importation of soybean will be continuously needed to fulfill the market and because SCN is absent in the soybean fields of Taiwan, lacking rhg1 resistance in the local soybean varieties may put the edamame industry at risk and early implementation of SCN resistance in the breeding program, alongside the application of quarantine regulations, will be the key to maintain the SCN-free status and to sustain the edamame industry in Taiwan.

scholarly journals iTRAQ-Based Proteomic Analysis Reveals the Role of the Biological Control Agent, Sinorhizobium fredii Strain Sneb183, in Enhancing Soybean Resistance Against the Soybean Cyst Nematode

2020 ◽  
Vol 11 ◽  
Author(s):  
Yuanyuan Wang ◽  
Ruowei Yang ◽  
Yaxing Feng ◽  
Aatika Sikandar ◽  
Xiaofeng Zhu ◽  
...  
Keyword(s):  
Biological Control ◽  
Disease Resistance ◽  
Soybean Cyst Nematode ◽  
Fermentation Broth ◽  
Cyst Nematode ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Sinorhizobium Fredii ◽  
Soybean Resistance ◽  
Control Samples

The soybean cyst nematode (SCN), Heterodera glycines Ichinohe, poses a serious threat to soybean production worldwide. Biological control agents have become eco-friendly candidates to control pathogens. Our previous study indicated that the biocontrol agent, Sinorhizobium fredii strain Sneb183, may induce soybean resistance to SCN. To study the mechanisms underlying induced disease resistance in the plant by Sneb183, an iTRAQ (isobaric tag for relative and absolute quantitation)-based proteomics approach was used to identify proteomic changes in SCN-infected soybean roots derived from seeds coated with the Sneb183 fermentation broth or water. Among a total of 456 identified differentially expressed proteins, 212 and 244 proteins were upregulated and downregulated, respectively, in Sneb183 treated samples in comparison to control samples. Some identified differentially expressed proteins are likely to be involved in the biosynthesis of phenylpropanoid, flavone, flavanol, and isoflavonoid and have a role in disease resistance and adaptation to environmental stresses. We used quantitative real-time PCR (qRT-PCR) to analyze key genes, including GmPAL (phenylalanine ammonia-lyase), GmCHR (chalcone reductase), GmCHS (chalcone synthase), and GmIFS (isoflavone synthase), that are involved in isoflavonoid biosynthesis in Sneb183-treated and control samples. The results showed that these targeted genes have higher expression levels in Sneb183-treated than in control samples. High performance liquid chromatography (HPLC) analysis further showed that the contents of daidzein in Sneb183-treated samples were 7.24 times higher than those in control samples. These results suggested that the Sinorhizobium fredii strain Sneb183 may have a role in inducing isoflavonoid biosynthesis, thereby resulting in enhanced resistance to SCN infection in soybean.

scholarly journals Inheritance pattern and selection criteria for resistance to soybean cyst nematode races 3 and 9

2007 ◽  
Vol 42 (10) ◽  
pp. 1413-1419 ◽  
Author(s):  
Gerardo Domingo Lucio Cervigni ◽  
Ivan Schuster ◽  
Carlos Sigueyuki Sediyama ◽  
Everaldo Gonçalves de Barros ◽  
Maurilio Alves Moreira
Keyword(s):  
Genetic Correlation ◽  
Soybean Cyst Nematode ◽  
Recombinant Inbred Lines ◽  
Indirect Selection ◽  
Cyst Nematode ◽  
Inheritance Pattern ◽  
Genetic Components ◽  
Randomized Design ◽  
Soybean Resistance ◽  
Completely Randomized Design

The objective of this work was to determine soybean resistance inheritance to Heterodera glycines Ichinohe (soybean cyst nematode - SCN) races 3 and 9, as well as to evaluate the efficiency of direct and indirect selection in a soybean population of 112 recombinant inbred lines (RIL) derived from the resistant cultivar Hartwig. The experiment was conducted in a completely randomized design, in Londrina, PR, Brazil. The estimated narrow-sense heritabilities for resistance to races 3 and 9 were 80.67 and 77.97%. The genetic correlation coefficient (r g = 0.17; p<0.01) shows that some genetic components of resistance to these two races are inherited together. The greatest genetic gain by indirect selection was obtained to race 9, selecting to race 3 due to simpler inheritance of resistance to race 9 and not because these two races share common resistance genes. The resistance of cultivar Hartwig to races 3 and 9 is determined by 4 and 2 genes, respectively. One of these genes confers resistance to both races, explaining a fraction of the significant genetic correlation found between resistance to these SCN races. The inheritance pattern described indicates that selection for resistance to SCN must be performed for each race individually.

scholarly journals Genetics and Adaptation of Soybean Cyst Nematode to Broad Spectrum Soybean Resistance

2017 ◽  
Vol 7 (3) ◽  
pp. 835-841 ◽  
Author(s):  
Michael Gardner ◽  
Robert Heinz ◽  
Jianying Wang ◽  
Melissa G. Mitchum
Keyword(s):  
Broad Spectrum ◽  
Soybean Cyst Nematode ◽  
Cyst Nematode ◽  
Soybean Resistance
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