scholarly journals The Wheat Ethylene Response Factor Transcription Factor PATHOGEN-INDUCED ERF1 Mediates Host Responses to Both the Necrotrophic Pathogen Rhizoctonia cerealis and Freezing Stresses

2014 ◽  
Vol 164 (3) ◽  
pp. 1499-1514 ◽  
Author(s):  
Xiuliang Zhu ◽  
Lin Qi ◽  
Xin Liu ◽  
Shibin Cai ◽  
Huijun Xu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Host Responses ◽  
Ethylene Response Factor ◽  
Response Factor ◽  
Necrotrophic Pathogen ◽  
Ethylene Response ◽  
Rhizoctonia Cerealis

scholarly journals Structural insights into DNA sequence recognition by Arabidopsis ETHYLENE RESPONSE FACTOR 96

2018 ◽  
Author(s):  
Chun-Yen Chen ◽  
Pei-Hsuan Lin ◽  
Kun-Hung Chen ◽  
Yi-Sheng Cheng
Keyword(s):  
Transcription Factor ◽  
Defense Responses ◽  
Structural Basis ◽  
Ethylene Response Factor ◽  
Response Factor ◽  
Recognition Sequence ◽  
Gcc Box ◽  
Sequence Recognition ◽  
Ethylene Response ◽  
Α Helix

ABSTRACTThe phytohormone ethylene is widely involved in many developmental processes and is a crucial regulator of defense responses against biotic and abiotic stresses in plants. Ethylene-responsive element binding protein (EREBP), a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis-acting element of target DNA. A previous study showed only the NMR structure of the AP2/ERF domain of AtERF100 in complex with a GCC box DNA motif. In this report, we determined the crystal structure of AtERF96 in complex with a GCC box at atomic resolution. We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. In addition to the AP2/ERF domain, an N-terminal α-helix of AtERF96 participates in DNA interaction in the flanking region. We also demonstrated the structure of AtERF96 EDLL motif, a unique conserved motif in the group IX of AP2/ERF family, is critical for the transactivation of defense-related genes. Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal α-helix and AP2/ERF domain.

scholarly journals Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box

2020 ◽  
Vol 104 (4-5) ◽  
pp. 483-498
Author(s):  
Chun-Yen Chen ◽  
Pei-Hsuan Lin ◽  
Kun-Hung Chen ◽  
Yi-Sheng Cheng
Keyword(s):  
Transcription Factor ◽  
Protein A ◽  
Defense Responses ◽  
Structural Basis ◽  
Ethylene Response Factor ◽  
Response Factor ◽  
Recognition Sequence ◽  
Gcc Box ◽  
Ethylene Response ◽  
Α Helix

Abstract The phytohormone ethylene is widely involved in many developmental processes and is a crucial regulator of defense responses against biotic and abiotic stresses in plants. Ethylene-responsive element binding protein, a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis-acting element of target DNA. A previous study showed only the NMR structure of the AP2/ERF domain of AtERF100 in complex with a GCC box DNA motif. In this report, we determined the crystal structure of AtERF96 in complex with a GCC box at atomic resolution. We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. In addition to the AP2/ERF domain, an N-terminal α-helix of AtERF96 participates in DNA interaction in the flanking region. We also demonstrated the structure of AtERF96 EDLL motif, a unique conserved motif in the group IX of AP2/ERF family, might involve in the transactivation of defense-related genes. Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal α-helix and AP2/ERF domain.

scholarly journals Role of ethylene and the APETALA 2/ethylene response factor superfamily in rice under various abiotic and biotic stress conditions

2017 ◽  
Vol 134 ◽  
pp. 33-44 ◽  
Author(s):  
Rambod Abiri ◽  
Noor Azmi Shaharuddin ◽  
Mahmood Maziah ◽  
Zetty Norhana Balia Yusof ◽  
Narges Atabaki ◽  
...  
Keyword(s):  
Biotic Stress ◽  
Stress Conditions ◽  
Ethylene Response Factor ◽  
Response Factor ◽  
Abiotic And Biotic Stress ◽  
Ethylene Response ◽  
Apetala 2

scholarly journals An AC-Rich Bean Element Serves as an Ethylene-Responsive Element in Arabidopsis

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1033
Author(s):  
Chunying Wang ◽  
Tingting Lin ◽  
Mengqi Wang ◽  
Xiaoting Qi
Keyword(s):  
Nuclear Proteins ◽  
Ethylene Response Factor ◽  
Response Factor ◽  
Cis Acting ◽  
Gcc Box ◽  
Ethylene Response ◽  
Significant Difference ◽  
Responsive Element ◽  
Transcription Factor 1

Ethylene-responsive elements (EREs), such as the GCC box, are critical for ethylene-regulated transcription in plants. Our previous work identified a 19-bp AC-rich element (ACE) in the promoter of bean (Phaseolus vulgaris) metal response element-binding transcription factor 1 (PvMTF-1). Ethylene response factor 15 (PvERF15) directly binds ACE to enhance PvMTF-1 expression. As a novel ERF-binding element, ACE exhibits a significant difference from the GCC box. Here, we demonstrated that ACE serves as an ERE in Arabidopsis. It conferred the minimal promoter to respond to the ethylene stress and inhibition of ethylene. Moreover, the cis-acting element ACE could specifically bind the nuclear proteins in vitro. We further revealed that the first 9-bp sequence of ACE (ACEcore) is importantly required by the binding of nuclear proteins. In addition, PvERF15 and PvMTF-1 were strongly induced by ethylene in bean seedlings. Since PvERF15 activates PvMTF-1 via ACE, ACE is involved in ethylene-induced PvMTF-1 expression. Taken together, our findings provide genetic and biochemical evidence for a new ERE.

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