scholarly journals RBF1, a Plant Homolog of the Bacterial Ribosome-Binding Factor RbfA, Acts in Processing of the Chloroplast 16S Ribosomal RNA

2013 ◽  
Vol 164 (1) ◽  
pp. 201-215 ◽  
Author(s):  
R. Fristedt ◽  
L. B. Scharff ◽  
C. A. Clarke ◽  
Q. Wang ◽  
C. Lin ◽  
...  
2004 ◽  
Vol 338 (4) ◽  
pp. 683-693 ◽  
Author(s):  
François Bélanger ◽  
Matthieu G. Gagnon ◽  
Sergey V. Steinberg ◽  
Philip R. Cunningham ◽  
Léa Brakier-Gingras

2019 ◽  
Vol 36 (12) ◽  
pp. 2854-2860 ◽  
Author(s):  
Iddo Weiner ◽  
Noam Shahar ◽  
Pini Marco ◽  
Iftach Yacoby ◽  
Tamir Tuller

Abstract Chloroplasts originated from an ancient cyanobacterium and still harbor a bacterial-like genome. However, the centrality of Shine–Dalgarno ribosome binding, which predominantly regulates proteobacterial translation initiation, is significantly decreased in chloroplasts. As plastid ribosomal RNA anti-Shine–Dalgarno elements are similar to their bacterial counterparts, these sites alone cannot explain this decline. By computational simulation we show that upstream point mutations modulate the local structure of ribosomal RNA in chloroplasts, creating significantly tighter structures around the anti-Shine–Dalgarno locus, which in-turn reduce the probability of ribosome binding. To validate our model, we expressed two reporter genes (mCherry, hydrogenase) harboring a Shine–Dalgarno motif in the Chlamydomonas reinhardtii chloroplast. Coexpressing them with a 16S ribosomal RNA, modified according to our model, significantly enhances mCherry and hydrogenase expression compared with coexpression with an endogenous 16S gene.


1991 ◽  
Vol 10 (8) ◽  
pp. 2203-2214 ◽  
Author(s):  
T. Powers ◽  
H.F. Noller

2003 ◽  
Vol 21 (3) ◽  
pp. 395-405 ◽  
Author(s):  
Moon K. Kim ◽  
Wen Li ◽  
Bruce A. Shapiro ◽  
Gregory S. Chirikjian

Author(s):  
Tina Felfeli ◽  
Felicia Tai ◽  
Peng Yan ◽  
Tony Mazzulli ◽  
Nupura K. Bakshi ◽  
...  

1985 ◽  
Vol 8 (3-4) ◽  
pp. 747-755 ◽  
Author(s):  
Harry F. Noller ◽  
Barbara J. Van Stolk ◽  
Danesh Moazed ◽  
Stephen Douthwaite ◽  
Robin R. Gutell

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