scholarly journals MULTI-FLORET SPIKELET1, Which Encodes an AP2/ERF Protein, Determines Spikelet Meristem Fate and Sterile Lemma Identity in Rice

2013 ◽  
Vol 162 (2) ◽  
pp. 872-884 ◽  
Author(s):  
Deyong Ren ◽  
Yunfeng Li ◽  
Fangming Zhao ◽  
Xianchun Sang ◽  
Junqiong Shi ◽  
...  
Keyword(s):  
Erf Protein ◽  
Sterile Lemma

scholarly journals LATERAL FLORET 1 induced the three-florets spikelet in rice

2017 ◽  
Vol 114 (37) ◽  
pp. 9984-9989 ◽  
Author(s):  
Ting Zhang ◽  
Yunfeng Li ◽  
Ling Ma ◽  
Xianchun Sang ◽  
Yinghua Ling ◽  
...  
Keyword(s):  
Leucine Zipper ◽  
Molecular Mechanisms ◽  
Ectopic Expression ◽  
Target Sequence ◽  
Class Iii ◽  
Rice Mutant ◽  
Inflorescence Structure ◽  
Meristem Maintenance ◽  
Floral Meristems ◽  
Sterile Lemma

The spikelet is a unique inflorescence structure in grass. The molecular mechanisms behind the development and evolution of the spikelet are far from clear. In this study, a dominant rice mutant, lateral florets 1 (lf1), was characterized. In the lf1 spikelet, lateral floral meristems were promoted unexpectedly and could generally blossom into relatively normal florets. LF1 encoded a class III homeodomain-leucine zipper (HD-ZIP III) protein, and the site of mutation in lf1 was located in a putative miRNA165/166 target sequence. Ectopic expression of both LF1 and the meristem maintenance gene OSH1 was detected in the axil of the sterile lemma primordia of the lf1 spikelet. Furthermore, the promoter of OSH1 could be bound directly by LF1 protein. Collectively, these results indicate that the mutation of LF1 induces ectopic expression of OSH1, which results in the initiation of lateral meristems to generate lateral florets in the axil of the sterile lemma. This study thus offers strong evidence in support of the “three-florets spikelet” hypothesis in rice.

scholarly journals Cloning of long sterile lemma (lsl2), a single recessive gene that regulates spike germination in rice (Oryza sativa L.)

2020 ◽  
Author(s):  
dewei yang ◽  
Niqing He ◽  
Xianghua Zheng ◽  
Yanmei Zhen ◽  
Zhenxin Xie ◽  
...  
Keyword(s):  
Seed Germination ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Gene Prediction ◽  
Germination Rate ◽  
Rice Genome ◽  
Recessive Gene ◽  
Floral Organ ◽  
Monocotyledonous Plant ◽  
Sterile Lemma

Abstract Background: Rice is a typical monocotyledonous plant and an important cereal crop. The structural units of rice flowers are spikelets and florets, and floral organ development and spike germination affect rice reproduction and yield.Results: In this study, we identified a novel long sterile lemma (lsl2) mutant from an EMS population. First, we mapped the lsl2 gene between the markers Indel7-22 and Indel7-27, which encompasses a 25-kb region. The rice genome annotation indicated the presence of four candidate genes in this region. Through gene prediction and cDNA sequencing, we confirmed that the target gene in the lsl2 mutant is allelic to LONG STERILE LEMMA1 (G1)/ELONGATED EMPTY GLUME (ELE), hereafter referred to as lsl2. Further analysis of the lsl2 and LSL2 proteins showed a one-amino-acid change, namely, the mutation of serine (Ser) 79 to proline (Pro) in lsl2 compared with LSL2, and this mutation might change the function of the protein. Knockout experiments showed that the lsl2 gene is responsible for the long sterile lemma phenotype. The lsl2 gene might reduce the damage induced by spike germination by decreasing the seed germination rate, but other agronomic traits of rice were not changed in the lsl2 mutant. Taken together, our results demonstrate that the lsl2 gene will have specific application prospects in future rice breeding.Conclusions: The lsl2 gene is responsible for the long sterile lemma phenotype and might reduce the damage induced by spike germination by decreasing the seed germination rate.

scholarly journals Cloning of long sterile lemma (lsl2), a single recessive gene that regulates spike germination in rice (Oryza sativa L.)

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Dewei Yang ◽  
Niqing He ◽  
Xianghua Zheng ◽  
Yanmei Zhen ◽  
Zhenxin Xie ◽  
...  
Keyword(s):  
Seed Germination ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Gene Prediction ◽  
Germination Rate ◽  
Rice Genome ◽  
Recessive Gene ◽  
Floral Organ ◽  
Monocotyledonous Plant ◽  
Sterile Lemma

Abstract Background Rice is a typical monocotyledonous plant and an important cereal crop. The structural units of rice flowers are spikelets and florets, and floral organ development and spike germination affect rice reproduction and yield. Results In this study, we identified a novel long sterile lemma (lsl2) mutant from an EMS population. First, we mapped the lsl2 gene between the markers Indel7–22 and Indel7–27, which encompasses a 25-kb region. The rice genome annotation indicated the presence of four candidate genes in this region. Through gene prediction and cDNA sequencing, we confirmed that the target gene in the lsl2 mutant is allelic to LONG STERILE LEMMA1 (G1)/ELONGATED EMPTY GLUME (ELE), hereafter referred to as lsl2. Further analysis of the lsl2 and LSL2 proteins showed a one-amino-acid change, namely, the mutation of serine (Ser) 79 to proline (Pro) in lsl2 compared with LSL2, and this mutation might change the function of the protein. Knockout experiments showed that the lsl2 gene is responsible for the long sterile lemma phenotype. The lsl2 gene might reduce the damage induced by spike germination by decreasing the seed germination rate, but other agronomic traits of rice were not changed in the lsl2 mutant. Taken together, our results demonstrate that the lsl2 gene will have specific application prospects in future rice breeding. Conclusions The lsl2 gene is responsible for the long sterile lemma phenotype and might reduce the damage induced by spike germination by decreasing the seed germination rate.

scholarly journals POACEAE: NOTES ON SPECIES OF EHRHARTA WITH A SHORT FIRST STERILE LEMMA

Bothalia ◽  
1984 ◽  
Vol 15 (1/2) ◽  
pp. 149-151
Author(s):  
G. E. Gibbs Russell
Keyword(s):  
Sterile Lemma

NOTES ON SPECIES OF  EHRHARTA WITH A SHORT FIRST STERILE LEMMA

scholarly journals Molecular cloning and characterization of GhERF105, a gene contributing to the regulation of gland formation in upland cotton (Gossypium hirsutum L.)

2021 ◽  
Author(s):  
Chaofeng Wu ◽  
Hailiang Cheng ◽  
Shuyan Li ◽  
Dongyun Zuo ◽  
Zhongxu Lin ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Pigment Gland ◽  
Human Beings ◽  
Virus Induced Gene Silencing ◽  
Ruminant Animals ◽  
Erf Protein ◽  
Glandless Seed ◽  
Ap2 Domain ◽  
Cotton Cultivar

Abstract Background: Gossypium hirsutum L. ( cotton) is one of the most economically important crops in the world due to its significant source of fiber, feed, foodstuff, oil and biofuel products. However, the utilization of cottonseed was limited due to the presence of small and darkly pigmented glands that contain large amounts of gossypol, which is toxic to human beings and non-ruminant animals. To date, some progress has been made in the pigment gland formation, but the underlying molecular mechanism of its formation was still unclear.Results: In this study, we identified an AP2/ERF transcription factor named GhERF105 (GH_A12G2166), which was involved in the regulation of gland pigmentation by the comparative transcriptome analysis of the leaf of glanded and glandless plants. It encoded an ERF protein containing a converved AP2 domain which was localized in the nucleus with transcriptional activity, and showed the high expression in glanded cotton accessions that contained much gossypol. Virus-induced gene silencing(VIGS) against GhERF105 caused the dramatic reduction in the number of glands and significantly lowered levels of gossypol in cotton leaves. GhERF105 showed the patterns of spatiotemporal and inducible expression in the glanded plants. Conclusions: These results suggest that GhERF105 contributes to the pigment gland formation and gossypol biosynthesis in partial organs of glanded plant. It also provides a potential molecular basis to generate ‘glandless-seed’ and ‘glanded-plant’ cotton cultivar.

scholarly journals Taxonomy of the genus Ehrharta (Poaceae) in southern Africa: the Setacea group

Bothalia ◽  
1987 ◽  
Vol 17 (1) ◽  
pp. 67-73 ◽  
Author(s):  
G. E. Gibbs Russell
Keyword(s):  
Southern Africa ◽  
Leaf Blade ◽  
Plant Size ◽  
Species Group ◽  
Western Cape ◽  
The South ◽  
Western Cape Province ◽  
Blade Width ◽  
Sterile Lemma

The Setacea species group in the genus Ehrharta Thunb. is differentiated morphologically by the short first sterile lemma and by inflorescences of fewer than 20 spikelets. The Setacea group is composed of two species, each with subspecies linked by intraspecific intermediates: E. rupestris Nees ex Trin. subsp.  rupestris; subsp.  tricostata (Stapf) Gibbs Russell; subsp.  dodii (Stapf) Gibbs Russell and E. setacea Nees subsp.  setacea; subsp.  scabra (Stapf) Gibbs Russell; subsp. uniflora (Burch, ex Stapf) Gibbs Russell; subsp.  disticha Gibbs Russell. All taxa are endemic to the Fynbos vegetation of the south-western Cape Province, with distribution centred in the Caledon degree grid (3419). Parallel trends for plant size and habit, leaf blade width and position, and spikelet size are demonstrated in both species, with similar plant types occurring in similar geographical areas.

scholarly journals Characterization and Gene Mapping of non-open hull 1 (noh1) Mutant in Rice (Oryza sativa L.)

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 56 ◽  
Author(s):  
Jun Zhang ◽  
Hao Zheng ◽  
Xiaoqin Zeng ◽  
Hui Zhuang ◽  
Honglei Wang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Recessive Gene ◽  
Physical Region ◽  
Chromosome 1 ◽  
Single Recessive Gene ◽  
Rice Mutant ◽  
Qpcr Analysis ◽  
Ethylmethane Sulfonate ◽  
Sterile Lemma

Hull opening is a key physiological process during reproductive development, strongly affecting the subsequent fertilization and seed development in rice. In this study, we characterized a rice mutant, non-open hull 1 (noh1), which was derived from ethylmethane-sulfonate (EMS)-treated Xinong 1B (Oryza sativa L.). All the spikelets of noh1 developed elongated and thin lodicules, which caused the failure of hull opening and the cleistogamy. In some spikelets of the noh1, sterile lemmas transformed into hull-like organs. qPCR analysis indicated that the expression of A- and E-function genes was significantly upregulated, while the expression of some B-function genes was downregulated in the lodicules of noh1. In addition, the expression of A-function genes was significantly upregulated, while the expression of some sterile-lemma maker genes was downregulated in the sterile lemma of noh1. These data suggested that the lodicule and sterile lemma in noh1 mutant gained glume-like and lemma-like identity, respectively. Genetic analysis showed that the noh1 trait was controlled by a single recessive gene. The NOH1 gene was mapped between the molecular markers ZJ-9 and ZJ-25 on chromosome 1 with a physical region of 60 kb, which contained nine annotated genes. These results provide a foundation for the cloning and functional research of NOH1 gene.

LRG1 maintains sterile lemma identity by regulating OsMADS6 expression in rice

2020 ◽  
Author(s):  
Qiankun Xu ◽  
Xiaoqi Yu ◽  
Yuanjiang Cui ◽  
Saisai Xia ◽  
Dali Zeng ◽  
...  
Keyword(s):  
Sterile Lemma
Sign in / Sign up

Export Citation Format

Share Document