scholarly journals Ubiquitin-Specific Proteases UBP12 and UBP13 Act in Circadian Clock and Photoperiodic Flowering Regulation in Arabidopsis

2013 ◽  
Vol 162 (2) ◽  
pp. 897-906 ◽  
Author(s):  
Xia Cui ◽  
Falong Lu ◽  
Yue Li ◽  
Yongming Xue ◽  
Yanyuan Kang ◽  
...  
Keyword(s):  
Circadian Clock ◽  
Photoperiodic Flowering ◽  
Flowering Regulation

scholarly journals FLOWERING HTH1 is involved in CONSTANS-mediated flowering regulation in Arabidopsis

2019 ◽  
Vol 62 (1) ◽  
Author(s):  
Soon Ae Sim ◽  
Su Gyeong Woo ◽  
Dae Yeon Hwang ◽  
Jin-Hong Kim ◽  
Seung Sik Lee ◽  
...  
Keyword(s):  
Subcellular Location ◽  
Day Length ◽  
Interacting Protein ◽  
Photoperiodic Flowering ◽  
Family Protein ◽  
Flowering Regulation ◽  
Delayed Flowering ◽  
The Right ◽  
Two Hybrid ◽  
Repression Domain

Abstract Flowering at the right time is essential for maximum reproductive fitness. In Arabidopsis thaliana, the CONSTANS (CO) protein facilitates the transition from the vegetative phase to the reproductive phase under long-day conditions. The formation of heterodimeric complexes between CO and DNA binding domain-containing transcription factors is important for the induction of day length-dependent flowering. Here, we report a myb-like helix turn helix (HTH) transcriptional regulator family protein as a new modulator of floral transition, which we have named FLOWERING HTH1 (FHTH1). We isolated FHTH1 as a CO-interacting protein by a yeast two-hybrid screen using an Arabidopsis transcription factor library. Our analysis showed that FHTH1 presented in the nucleus and the FHTH1-CO complex was formed in the same subcellular location. We also observed the expression of a FHTH1:GUS construct in the leaf vasculature, where CO exists. Transgenic plants overexpressing FHTH1 fused with the plant-specific repression domain SRDX showed a delayed flowering phenotype in long days, resembling the phenotype of the co mutant. Our results suggest that FHTH1 may contribute to CO-mediated photoperiodic flowering regulation.

scholarly journals Conserved Expression Profiles of Circadian Clock-related Genes in Two Lemna Species Showing Long-day and Short-day Photoperiodic Flowering Responses

2006 ◽  
Vol 47 (5) ◽  
pp. 601-612 ◽  
Author(s):  
Kumiko Miwa ◽  
Masayuki Serikawa ◽  
Sayaka Suzuki ◽  
Takao Kondo ◽  
Tokitaka Oyama
Keyword(s):  
Circadian Clock ◽  
Expression Profiles ◽  
Short Day ◽  
Photoperiodic Flowering ◽  
Long Day

scholarly journals Pea LATE BLOOMER1 Is a GIGANTEA Ortholog with Roles in Photoperiodic Flowering, Deetiolation, and Transcriptional Regulation of Circadian Clock Gene Homologs

2007 ◽  
Vol 144 (2) ◽  
pp. 648-661 ◽  
Author(s):  
Valérie Hecht ◽  
Claire L. Knowles ◽  
Jacqueline K. Vander Schoor ◽  
Lim Chee Liew ◽  
Sarah E. Jones ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Circadian Clock ◽  
Clock Gene ◽  
Circadian Clock Gene ◽  
Photoperiodic Flowering

Circadian clock and photoperiodic flowering genes in adzuki bean (Vigna angularis [Willd.] Ohwi & H. Ohashi)

2014 ◽  
Vol 12 (S1) ◽  
pp. S49-S53 ◽  
Author(s):  
Moon Young Kim ◽  
Yang Jae Kang ◽  
Taeyoung Lee ◽  
Suk-Ha Lee
Keyword(s):  
Circadian Clock ◽  
Environmental Changes ◽  
De Novo ◽  
Agronomic Traits ◽  
Sequence Information ◽  
Adzuki Bean ◽  
Vigna Angularis ◽  
Protein Coding ◽  
Flowering Genes ◽  
Photoperiodic Flowering

Adzuki bean (Vigna angularis [Willd.] Ohwi & H. Ohashi) is one of the most important legume crops cultivated in East Asia and northern South Asia. Despite its agronomic importance, the lack of available sequence information has made it difficult to improve important agronomic traits. In the present study, we performed de novo assembly of transcript sequences produced by short-read sequencing to construct 59,860 full-length protein-coding sequences in adzuki bean. These genes were subjected to a BLASTP search to identify putative homologues of the 84 Arabidopsis genes involved in the circadian clock and photoperiodic flowering pathway. A large proportion of these Arabidopsis genes were found to be conserved in adzuki bean. However, there were no homologues of six genes including FLOWER LOCUS D (FD) and LEAFY (LFY). Furthermore, the phylogenetic relationships of 25 highly homologous matches to CONSTANS (CO) or CONSTANS-LIKE (COL) of Arabidopsis indicated the lack of a CO orthologue in adzuki bean. FLOWER LOCUS T (FT) and its homologues were found to have two homologous counterparts in adzuki bean. This study provides primary genetic resources that may be useful for producing adzuki bean with improved flowering and fruiting performance in response to environmental changes.

scholarly journals Photoperiod sensing of the circadian clock is controlled by EARLY FLOWERING 3 and GIGANTEA

2018 ◽  
Author(s):  
Muhammad Usman Anwer ◽  
Amanda Davis ◽  
Seth Jon Davis ◽  
Marcel Quint
Keyword(s):  
Circadian Clock ◽  
Double Mutant ◽  
Genetic Interaction ◽  
Loss Of Function ◽  
Cellular Mechanisms ◽  
Environmental Signals ◽  
Photoperiodic Flowering ◽  
Oscillator Function ◽  
Key Genes ◽  
Central Oscillator

SummaryELF3 and GI are two important components of the Arabidopsis circadian clock. They are not only essential for the oscillator function but are also pivotal in mediating light inputs to the oscillator. Lack of either results in a defective oscillator causing severely compromised output pathways, such as photoperiodic flowering and hypocotyl elongation. Although single loss of function mutants of ELF3 and GI have been well-studied, their genetic interaction remains unclear. We generated an elf3 gi double mutant to study their genetic relationship in clock-controlled growth and phase transition phenotypes. We found that ELF3 and GI repress growth differentially during the night and the day, respectively. Circadian clock assays revealed that ELF3 and GI are essential Zeitnehmers that enable the oscillator to synchronize the endogenous cellular mechanisms to external environmental signals. In their absence, the circadian oscillator fails to synchronize to the light-dark cycles even under diurnal conditions. Consequently, clock-mediated photoperiod-responsive growth and development is completely lost in plants lacking both genes, suggesting that ELF3 and GI together convey photoperiod sensing to the central oscillator. Since ELF3 and GI are conserved across flowering plants and represent important breeding and domestication targets, our data highlight the possibility of developing photoperiod-insensitive crops by adjusting the allelic combination of these two key genes.One sentence summaryELF3 and GI are essential for circadian clock mediated photoperiod sensing.

scholarly journals A point mutation in Phytochromobilin Synthase alters the circadian clock and photoperiodic flowering of Medicago truncatula

2021 ◽  
Author(s):  
Soledad Perez Santangelo ◽  
Nathanael Napier ◽  
Fran Robson ◽  
James Weller ◽  
Donna Bond ◽  
...  
Keyword(s):  
Circadian Clock ◽  
Medicago Truncatula ◽  
Clock Genes ◽  
Light Signaling ◽  
Circadian Period ◽  
Crop Species ◽  
Light Sensing ◽  
Tilling Population ◽  
Photoperiodic Flowering ◽  
Time Of Year

Plants use seasonal cues to initiate flowering at an appropriate time of year to ensure optimal reproductive success. The circadian clock integrates these daily and seasonal cues with internal cues to initiate flowering. The molecular pathways that control the sensitivity of flowering to photoperiod (daylength) are well described in the model plant Arabidopsis. However, much less is known in crop species, such as the legume family species. Here we performed a flowering time screen of a TILLING population of Medicago truncatula and found a line with late-flowering and altered light-sensing phenotypes. Using RNA-sequencing, we identified a nonsense mutation in the Phytochromobilin Synthase (MtPΦBS) gene, which encodes an enzyme that carries out the final step in the biosynthesis of the chromophore required for phytochrome (PHY) activity. The analysis of the circadian clock in the MtpΦbs mutant revealed a shorter circadian period, which was shared with the phyA mutant. The MtpΦbs and MtphyA mutants showed downregulation of FT floral regulators MtFTa1, MtFTb1/b2 and a shift in phase for morning and night core clock genes. Our findings show that PHYA is necessary to synchronize the circadian clock and integration of light signaling to promote expression of the MtFT genes to precisely time flowering.

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