scholarly journals Truncated Photosystem Chlorophyll Antenna Size in the Green Microalga Chlamydomonas reinhardtii upon Deletion of the TLA3-CpSRP43 Gene

2012 ◽  
Vol 160 (4) ◽  
pp. 2251-2260 ◽  
Author(s):  
Henning Kirst ◽  
Jose Gines Garcia-Cerdan ◽  
Andreas Zurbriggen ◽  
Thilo Ruehle ◽  
Anastasios Melis
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Antenna Size ◽  
Green Microalga ◽  
Chlorophyll Antenna Size

scholarly journals Modulation of the light-harvesting chlorophyll antenna size in Chlamydomonas reinhardtii by TLA1 gene over-expression and RNA interference

2012 ◽  
Vol 367 (1608) ◽  
pp. 3430-3443 ◽  
Author(s):  
Mautusi Mitra ◽  
Henning Kirst ◽  
David Dewez ◽  
Anastasios Melis
Keyword(s):  
Gene Expression ◽  
Chlamydomonas Reinhardtii ◽  
Light Harvesting ◽  
Thylakoid Membranes ◽  
Chloroplast Envelope ◽  
Cell Fractionation ◽  
Reaction Centre ◽  
Antenna Size ◽  
Over Expression ◽  
Chlorophyll Antenna Size

Truncated light-harvesting antenna 1 ( TLA1 ) is a nuclear gene proposed to regulate the chlorophyll (Chl) antenna size in Chlamydomonas reinhardtii . The Chl antenna size of the photosystems and the chloroplast ultrastructure were manipulated upon TLA1 gene over-expression and RNAi downregulation. The TLA1 over-expressing lines possessed a larger chlorophyll antenna size for both photosystems and contained greater levels of Chl b per cell relative to the wild type. Conversely, TLA1 RNAi transformants had a smaller Chl antenna size for both photosystems and lower levels of Chl b per cell. Western blot analyses of the TLA1 over-expressing and RNAi transformants showed that modulation of TLA1 gene expression was paralleled by modulation in the expression of light-harvesting protein, reaction centre D1 and D2, and VIPP1 genes. Transmission electron microscopy showed that modulation of TLA1 gene expression impacts the organization of thylakoid membranes in the chloroplast. Over-expressing lines showed well-defined grana, whereas RNAi transformants possessed loosely held together and more stroma-exposed thylakoids. Cell fractionation suggested localization of the TLA1 protein in the inner chloroplast envelope and potentially in association with nascent thylakoid membranes, indicating a role in Chl antenna assembly and thylakoid membrane biogenesis. The results provide a mechanistic understanding of the Chl antenna size regulation by the TLA1 gene.

scholarly journals Introns mediate post-transcriptional enhancement of nuclear gene expression in the green microalga Chlamydomonas reinhardtii

PLoS Genetics ◽  
2020 ◽  
Vol 16 (7) ◽  
pp. e1008944 ◽  
Author(s):  
Thomas Baier ◽  
Nick Jacobebbinghaus ◽  
Alexander Einhaus ◽  
Kyle J. Lauersen ◽  
Olaf Kruse
Keyword(s):  
Gene Expression ◽  
Chlamydomonas Reinhardtii ◽  
Nuclear Gene ◽  
Nuclear Gene Expression ◽  
Green Microalga

Functional analysis of three type-2 DGAT homologue genes for triacylglycerol production in the green microalga Chlamydomonas reinhardtii

2012 ◽  
Vol 162 (1) ◽  
pp. 13-20 ◽  
Author(s):  
M. La Russa ◽  
C. Bogen ◽  
A. Uhmeyer ◽  
A. Doebbe ◽  
E. Filippone ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Chlamydomonas Reinhardtii ◽  
Green Microalga

scholarly journals Loss of CpSRP54 function leads to a truncated light-harvesting antenna size in Chlamydomonas reinhardtii

2017 ◽  
Vol 1858 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Jooyeon Jeong ◽  
Kwangryul Baek ◽  
Henning Kirst ◽  
Anastasios Melis ◽  
EonSeon Jin
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Light Harvesting ◽  
Antenna Size ◽  
Light Harvesting Antenna
Sign in / Sign up

Export Citation Format

Share Document