scholarly journals An A/ENTH Domain-Containing Protein Functions as an Adaptor for Clathrin-Coated Vesicles on the Growing Cell Plate in Arabidopsis Root Cells

2012 ◽  
Vol 159 (3) ◽  
pp. 1013-1025 ◽  
Author(s):  
Kyungyoung Song ◽  
Mihue Jang ◽  
Soo Youn Kim ◽  
Goeun Lee ◽  
Gil-Je Lee ◽  
...  
Keyword(s):  
Cell Plate ◽  
Coated Vesicles ◽  
Root Cells ◽  
Arabidopsis Root ◽  
Enth Domain ◽  
Growing Cell ◽  
Protein Functions

scholarly journals Co-ordination and divergence of cell-specific transcription and translation of genes in arabidopsis root cells

2014 ◽  
Vol 114 (6) ◽  
pp. 1109-1123 ◽  
Author(s):  
Dhivyaa Rajasundaram ◽  
Joachim Selbig ◽  
Staffan Persson ◽  
Sebastian Klie
Keyword(s):  
Root Cells ◽  
Arabidopsis Root

scholarly journals Analyses of Ca2+ Accumulation and Dynamics in the Endoplasmic Reticulum of Arabidopsis Root Cells Using a Genetically Encoded Cameleon Sensor

2013 ◽  
Vol 163 (3) ◽  
pp. 1230-1241 ◽  
Author(s):  
Maria Cristina Bonza ◽  
Giovanna Loro ◽  
Smrutisanjita Behera ◽  
Andrea Wong ◽  
Jörg Kudla ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Root Cells ◽  
Arabidopsis Root

scholarly journals Clint: A Novel Clathrin-binding ENTH-Domain Protein at the Golgi

2002 ◽  
Vol 13 (11) ◽  
pp. 4060-4073 ◽  
Author(s):  
Christoph Kalthoff ◽  
Stephanie Groos ◽  
Rüdiger Kohl ◽  
Stefan Mahrhold ◽  
Ernst J. Ungewickell
Keyword(s):  
Epithelial Cells ◽  
Collecting Duct ◽  
Rat Kidney ◽  
Coated Vesicles ◽  
Apical Region ◽  
Interacting Protein ◽  
Enth Domain ◽  
Rich Domain ◽  
Golgi Region ◽  
Domain Protein

We have characterized a novel clathrin-binding 68-kDa epsin N-terminal homology domain (ENTH-domain) protein that we name clathrin interacting protein localized in the trans-Golgi region (Clint). It localizes predominantly to the Golgi region of epithelial cells as well as to more peripheral vesicular structures. Clint colocalizes with AP-1 and clathrin only in the perinuclear area. Recombinantly expressed Clint interacts directly with the γ-appendage domain of AP-1, with the clathrin N-terminal domain through the peptide motif 423LFDLM, with the γ-adaptin ear homology domain of Golgi-localizing, γ-adaptin ear homology domain 2, with the appendage domain of β2-adaptin and to a lesser extent with the appendage domain of α-adaptin. Moreover, the Clint ENTH-domain asssociates with phosphoinositide-containing liposomes. A significant amount of Clint copurifies with rat liver clathrin-coated vesicles. In rat kidney it is preferentially expressed in the apical region of epithelial cells that line the collecting duct. Clathrin and Clint also colocalize in the apical region of enterocytes along the villi of the small intestine. Apart from the ENTH-domain Clint has no similarities with the epsins AP180/CALM or Hip1/1R. A notable feature of Clint is a carboxyl-terminal methionine-rich domain (Met427-Met605), which contains >17% methionine. Our results suggest that Clint might participate in the formation of clathrin-coated vesicles at the level of thetrans-Golgi network and remains associated with the vesicles longer than clathrin and adaptors.

Glutamate activates cation currents in the plasma membrane of Arabidopsis root cells

Planta ◽  
2004 ◽  
Vol 219 (1) ◽  
pp. 167-175 ◽  
Author(s):  
Vadim Demidchik ◽  
Pauline Adobea Essah ◽  
Mark Tester
Keyword(s):  
Plasma Membrane ◽  
Root Cells ◽  
Arabidopsis Root

scholarly journals Cytokinesis in fra2 Arabidopsis thaliana p60-katanin Mutant: Defects in Cell Plate/Daughter Wall Formation

2021 ◽  
Author(s):  
Emmanuel Panteris ◽  
Anna Kouskouveli ◽  
Dimitris Pappas ◽  
Ioannis-Dimosthenis S. Adamakis
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Cell Walls ◽  
Higher Plants ◽  
Cell Plate ◽  
Wild Type ◽  
Microtubule Organization ◽  
Root Cells ◽  
Wall Formation ◽  
In The Wild

Cytokinesis is accomplished in higher plants by the phragmoplast, creating and conducting the cell plate, to separate daughter nuclei by a new cell wall. The microtubule-severing enzyme p60-katanin plays an important role in the centrifugal expansion and timely disappearance of phragmoplast microtubules. Consequently, aberrant structure and delayed expansion rate of the phragmoplast occur in p60-katanin mutants. Here, the consequences of p60-katanin malfunction in cell plate/daughter wall formation were investigated by transmission electron microscopy (TEM), while deviations in the chemical composition of cell plate/new cell wall were identified by immunolabeling and confocal microscopy, in root cells of the fra2 Arabidopsis thaliana mutant. It was found that, apart from defective phragmoplast microtubule organization, cell plates/new cell walls appeared also faulty in structure, being unevenly thick and perforated by large gaps. In addition, demethylesterified homogalacturonans were prematurely present in fra2 cell plates, while callose content was significantly lower than in the wild-type. Furthermore, KNOLLE syntaxin disappeared from newly formed cell walls in fra2 earlier than in the wild-type. Taken together, these observations indicate that delayed cytokinesis, due to faulty phragmoplast organization and expansion, results in a loss of synchronization between cell plate growth and its chemical maturation.

scholarly journals Under phosphate starvation conditions, Fe and Al trigger accumulation of the transcription factor STOP1 in the nucleus of Arabidopsis root cells

2019 ◽  
Vol 99 (5) ◽  
pp. 937-949 ◽  
Author(s):  
Christian Godon ◽  
Caroline Mercier ◽  
Xiaoyue Wang ◽  
Pascale David ◽  
Pierre Richaud ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Phosphate Starvation ◽  
Root Cells ◽  
Arabidopsis Root ◽  
Starvation Conditions

The association of peroxisomes with the developing cell plate in dividing onion root cells depends on actin microfilaments and myosin

Planta ◽  
2003 ◽  
Vol 218 (2) ◽  
pp. 204-216 ◽  
Author(s):  
David A. Collings ◽  
John D. I. Harper ◽  
Kevin C. Vaughn
Keyword(s):  
Cell Plate ◽  
Actin Microfilaments ◽  
Root Cells ◽  
Onion Root
Sign in / Sign up

Export Citation Format

Share Document