scholarly journals A Constitutive Expressed Phosphate Transporter, OsPht1;1, Modulates Phosphate Uptake and Translocation in Phosphate-Replete Rice

2012 ◽  
Vol 159 (4) ◽  
pp. 1571-1581 ◽  
Author(s):  
Shubin Sun ◽  
Mian Gu ◽  
Yue Cao ◽  
Xinpeng Huang ◽  
Xiao Zhang ◽  
...  
Keyword(s):  
Phosphate Uptake ◽  
Phosphate Transporter

scholarly journals Disruption of Histone Deacetylase Gene RPD3 Accelerates PHO5 Activation Kinetics through Inappropriate Pho84p Recycling

2005 ◽  
Vol 4 (8) ◽  
pp. 1387-1395 ◽  
Author(s):  
Sriwan Wongwisansri ◽  
Paul J. Laybourn
Keyword(s):  
Histone Deacetylase ◽  
Cytoplasmic Membrane ◽  
Phosphate Uptake ◽  
Transcriptional Repressor ◽  
Phosphate Starvation ◽  
Phosphate Transporter ◽  
Regulatory Pathway ◽  
Activation Kinetics ◽  
High Affinity ◽  
Negative Role

ABSTRACT The histone deacetylase Rpd3p functions as a transcriptional repressor of a diverse set of genes, including PHO5. Here we describe a novel role for RPD3 in the regulation of phosphate transporter Pho84p retention in the cytoplasmic membrane. We show that under repressing conditions (with Pi), PHO5 expression is increased in a pho4Δ rpd3Δ strain, demonstrating PHO regulatory pathway independence. However, the effect of RPD3 disruption on PHO5 activation kinetics is dependent on the PHO regulatory pathway. Upon switching to activating conditions (without Pi), PHO5 transcripts accumulated more rapidly in rpd3Δ cells. This more rapid response correlates with a defect in phosphate uptake due to premature recycling of Pho84p, the high-affinity H+/PO4 3− symporter. Thus, RPD3 also participates in PHO5 regulation through a previously unidentified effect on maintenance of high-affinity phosphate uptake during phosphate starvation. We propose that Rpd3p has a negative role in the regulation of Pho84p endocytosis.

A Brassica napus PHT1 phosphate transporter, BnPht1;4, promotes phosphate uptake and affects roots architecture of transgenic Arabidopsis

2014 ◽  
Vol 86 (6) ◽  
pp. 595-607 ◽  
Author(s):  
Feng Ren ◽  
Cai-Zhi Zhao ◽  
Chun-Sen Liu ◽  
Ke-Lin Huang ◽  
Qian-Qian Guo ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Transgenic Arabidopsis ◽  
Phosphate Uptake ◽  
Phosphate Transporter

scholarly journals Acquisition of the Phosphate Transporter NptA Enhances Staphylococcus aureus Pathogenesis by Improving Phosphate Uptake in Divergent Environments

2017 ◽  
Vol 86 (1) ◽  
Author(s):  
Jessica L. Kelliher ◽  
Jana N. Radin ◽  
Kyle P. Grim ◽  
Paola K. Párraga Solórzano ◽  
Patrick H. Degnan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Mouse Model ◽  
Inorganic Phosphate ◽  
Phosphate Uptake ◽  
Genomic Analysis ◽  
Phosphate Transporter ◽  
Phosphate Limitation ◽  
Content Type ◽  
Staphylococcal Species

ABSTRACTDuring infection, pathogens must obtain all inorganic nutrients, such as phosphate, from the host. Despite the essentiality of phosphate for all forms of life, howStaphylococcus aureusobtains this nutrient during infection is unknown. Differing fromEscherichia coli, the paradigm for bacterial phosphate acquisition, which has two inorganic phosphate (Pi) importers, genomic analysis suggested thatS. aureuspossesses three distinct Pitransporters: PstSCAB, PitA, and NptA. WhilepitAandnptAare expressed in phosphate-replete media, expression of all three transporters is induced by phosphate limitation. The loss of a single transporter did not affectS. aureus. However, disruption of any two systems significantly reduced Piaccumulation and growth in divergent environments. These findings indicate that PstSCAB, PitA, and NptA have overlapping but nonredundant functions, thus expanding the environments in whichS. aureuscan successfully obtain Pi. Consistent with this idea, in a systemic mouse model of disease, loss of any one transporter did not decrease staphylococcal virulence. However, loss of NptA in conjunction with either PstSCAB or PitA significantly reduced the ability ofS. aureusto cause infection. These observations suggest that Piacquisition via NptA is particularly important for the pathogenesis ofS. aureus. While our analysis suggests that NptA homologs are widely distributed among bacteria, closely related less pathogenic staphylococcal species do not possess this importer. Altogether, these observations indicate that Piuptake byS. aureusdiffers from established models and that acquisition of a third transporter enhances the ability of the bacterium to cause infection.

scholarly journals Evidence of an intestinal phosphate transporter alternative to type IIb sodium-dependent phosphate transporter in rats with chronic kidney disease

2020 ◽  
Vol 36 (1) ◽  
pp. 68-75
Author(s):  
Yasuhiro Ichida ◽  
Shuichi Ohtomo ◽  
Tessai Yamamoto ◽  
Naoaki Murao ◽  
Yoshinori Tsuboi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Mrna Expression ◽  
Messenger Rna ◽  
Phosphate Uptake ◽  
Maximum Velocity ◽  
Phosphate Transport ◽  
Phosphate Transporter ◽  
Sodium Dependent

Abstract Background Phosphate is absorbed in the small intestine via passive flow and active transport.NaPi-IIb, a type II sodium-dependent phosphate transporter, is considered to mediate active phosphate transport in rodents. To study the regulation of intestinal phosphate transport in chronic kidney disease (CKD), we analyzed the expression levels of NaPi-IIb, pituitary-specific transcription factor 1 (PiT-1) and PiT-2 and the kinetics of intestinal phosphate transport using two CKD models. Methods CKD was induced in rats via adenine orThy1 antibody injection. Phosphate uptake by intestinal brush border membrane vesicles (BBMV) and the messenger RNA (mRNA) expression of NaPi-IIb, PiT-1 and PiT-2 were analyzed. The protein expression level of NaPi-IIb was measured by mass spectrometry (e.g. liquid chromatography tandem mass spectrometry). Results In normal rats, phosphate uptake into BBMV consisted of a single saturable component and its Michaelis constant (Km) was comparable to that of NaPi-IIb. The maximum velocity (Vmax) correlated with mRNA and protein levels of NaPi-IIb. In the CKD models, intestinal phosphate uptake consisted of two saturable components. The Vmax of the higher-affinity transport, which is thought to be responsible for NaPi-IIb, significantly decreased and the decrease correlated with reduced NaPi-IIb expression. The Km of the lower-affinity transport was comparable to that of PiT-1 and -2. PiT-1 mRNA expression was much higher than that of PiT-2, suggesting that PiT-1 was mostly responsible for phosphate transport. Conclusions This study suggests that the contribution of NaPi-IIb to intestinal phosphate absorption dramatically decreases in rats with CKD and that a low-affinity alternative to NaPi-IIb, in particular PiT-1, is upregulated in a compensatory manner in CKD.

Uptake of phosphate by two cyanophytes: cation effects and energetics

1987 ◽  
Vol 65 (9) ◽  
pp. 1901-1907 ◽  
Author(s):  
K. Budd ◽  
G. W. Kerson
Keyword(s):  
Membrane Potential ◽  
Phosphate Uptake ◽  
Maximum Velocity ◽  
Proton Motive Force ◽  
Phosphate Transporter ◽  
Saturation Concentration ◽  
Cation Effects ◽  
Synechococcus Leopoliensis ◽  
Metallic Cations ◽  
Significant Uptake

Phosphate uptake in the cyanophytes Synechococcus leopoliensis and Oscillatoria limnetica did not require exogenous metallic cations. In O. limnetica phosphate uptake was described by a single Michaelis–Menten relationship between 0.2 and 10.0 μM phosphate. Calcium increased the maximum velocity and decreased the half-saturation concentration for this system; phosphate uptake was also enhanced by Sr2+ and by Mg2+ but not by Na+, K+, or Zn2+ at up to 1 mM. Calcium appeared to act upon the phosphate transporter and its action is suggested to be ecologically significant. Uptake of phosphate in the light by both cyanophytes was unaffected by 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) but was abolished by carbonylcyanide m-chlorophenylhydrazone (CCCP) or dicyclohexylcarbodiimide (DCCD) at concentrations that abolished apparent photosynthesis; however, phosphate uptake was not inhibited by concentrations of CCCP or DCCD that were only slightly inhibitory to apparent photosynthesis. At 5 μM, CCCP abolished light hyperpolarization of the membrane potential but was noninhibitory to phosphate uptake. Phosphate uptake in O. limnetica was accompanied by hyperpolarization of the membrane potential. Gramicidin hyperpolarized the membrane but did not influence phosphate uptake. It is suggested that phosphate uptake in both cyanophytes is independent of the proton motive force.

Transgenic Rice Overexperessing a Tomato Mitochondrial Phosphate Transporter, SlMPT3;1, Promotes Phosphate Uptake and Increases Grain Yield

2018 ◽  
Vol 61 (6) ◽  
pp. 383-400 ◽  
Author(s):  
Guo-hong Yu ◽  
Sheng-cai Huang ◽  
Rui He ◽  
Ying-zhang Li ◽  
Xian-guo Cheng
Keyword(s):  
Grain Yield ◽  
Transgenic Rice ◽  
Phosphate Uptake ◽  
Phosphate Transporter

Over-expression of a high-affinity phosphate transporter in transgenic barley plants does not enhance phosphate uptake rates

2004 ◽  
Vol 31 (2) ◽  
pp. 141 ◽  
Author(s):  
Anne L. Rae ◽  
Janine M. Jarmey ◽  
Stephen R. Mudge ◽  
Frank W. Smith
Keyword(s):  
Phosphate Uptake ◽  
Phosphate Concentration ◽  
Phosphate Transporter ◽  
Control Line ◽  
Wild Type ◽  
Gene Encoding ◽  
Over Expression ◽  
Transgenic Barley ◽  
Uptake Rates ◽  
Homozygous Line

Transgenic barley plants that over-express the gene encoding a phosphate transporter were generated and used to test the hypothesis that manipulation of transporters may lead to improved phosphate uptake by plant roots. Replicate T2 seedlings from a homozygous line with a single locus insertion were grown in dilute flow culture. The phosphate contents and uptake rates of these plants were compared with control transgenic and wild-type plants. When external phosphate concentration was maintained at 10 μM, all plants including the transgenic over-expressing line displayed low rates of phosphate uptake and contained high levels of phosphate in the shoot tissue. When external phosphate concentration was maintained at 2 μM, the uptake rates increased to a similar level in all plant lines. Three transgenic over-expressing lines were then grown in soil at a range of phosphate concentrations and the dry weights and total phosphorus contents of the shoots were measured and compared to a transgenic control line. The results showed that over-expression of the gene encoding a phosphate transporter did not improve the uptake of phosphate under any of the conditions tested. Transporter activity is likely to be influenced by post-transcriptional mechanisms and will require further investigation before this strategy can be applied to improving plant nutrition.

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