scholarly journals Ectopic Expression of Rubisco Subunits in Maize Mesophyll Cells Does Not Overcome Barriers to Cell Type-Specific Accumulation

2012 ◽  
Vol 160 (1) ◽  
pp. 419-432 ◽  
Author(s):  
Katia Wostrikoff ◽  
Aimee Clark ◽  
Shirley Sato ◽  
Tom Clemente ◽  
David Stern
Keyword(s):  
Ectopic Expression ◽  
Cell Type ◽  
Mesophyll Cells ◽  
Specific Accumulation ◽  
Cell Type Specific ◽  
Rubisco Subunits

scholarly journals Cell type specific accumulation of the major latency-associated transcript (LAT) of herpes simplex virus type 2 in LAT transgenic mice

Virology ◽  
2009 ◽  
Vol 386 (1) ◽  
pp. 79-87 ◽  
Author(s):  
Kening Wang ◽  
Gowtham Mahalingam ◽  
Yumi Imai ◽  
Lesley Pesnicak ◽  
Todd T. Margolis ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Transgenic Mice ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Cell Type ◽  
Specific Accumulation ◽  
Cell Type Specific ◽  
Simplex Virus

scholarly journals C-reactive protein is expressed in a cell-type specific manner in the murine thymus.

2020 ◽  
Author(s):  
Shahan Mamoor
Keyword(s):  
Inflammatory Responses ◽  
Ectopic Expression ◽  
Cell Type ◽  
Mammalian Development ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Specific Manner ◽  
A Cell ◽  
Cell Type Specific ◽  
Self Antigen

C-reactive protein, or CRP, is an acute phase protein (1, 2) synthesized and released from the liver (3). CRP is transcriptionally induced during systemic inflammatory responses (1, 2). CRP expression in the thymus has previously been reported but in the context of promiscuous gene expression of self-antigen during negative selection (4, 5) or after ectopic expression (6). Here, by comparing the transcriptomes of mTEC and cTEC from the thymuses of mice at 1, 3 and 6 months using a published dataset (7), we found that CRP was among the genes whose expression changed most significantly between cTEC and mTEC at 3 months of murine life. CRP was expressed at significantly higher amounts in mTEC compared to cTEC. Thus, CRP, a molecule typically thought of as expressed by the liver and induced during systemic inflammatory responses, is expressed in a cell-type specific manner during mammalian development in the thymus.

Correlative Light Electron Ion Microscopy reveal in vivo localisation of bedaquiline in Mycobacterium tuberculosis infected lungs

2020 ◽  
Author(s):  
Antony Fearns ◽  
Daniel J. Greenwood ◽  
Angela Rodgers ◽  
Haibo Jiang ◽  
Maximiliano G. Gutierrez
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Infected Tissue ◽  
Cell Type ◽  
Lung Lesions ◽  
Specific Accumulation ◽  
Cell Type Specific ◽  
Ion Microscopy ◽  
Foamy Macrophages ◽  
Intracellular Fate

AbstractCorrelative light, electron and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, we developed CLEIM in tissue (CLEIMiT), and used it to identify the cell-type specific accumulation of an antibiotic in lung lesions of mice infected with Mycobacterium tuberculosis. Using CLEIMiT, we found that the anti-TB drug bedaquiline is localised not only in foamy macrophages in the lungs during infection but also accumulate in polymorphonuclear (PMN) cells.

scholarly journals Expression Patterns of Cell-type–specific Genes inDictyostelium

2001 ◽  
Vol 12 (9) ◽  
pp. 2590-2600 ◽  
Author(s):  
Negin Iranfar ◽  
Danny Fuller ◽  
Roman Sasik ◽  
Terence Hwa ◽  
Michael Laub ◽  
...  
Keyword(s):  
Kinetic Equation ◽  
Dna Microarrays ◽  
Expression Profiles ◽  
Expression Patterns ◽  
The Other ◽  
Cell Type ◽  
Early Genes ◽  
Specific Accumulation ◽  
Cell Type Specific ◽  
The Times

Cell-type specific genes were recognized by interrogating microarrays carrying Dictyostelium gene fragments with probes prepared from fractions enriched in prestalk and prespore cells. Cell-type specific accumulation of mRNA from 17 newly identified genes was confirmed by Northern analyses. DNA microarrays carrying 690 targets were used to determine expression profiles during development. The profiles were fit to a biologically based kinetic equation to extract the times of transcription onset and cessation. Although the majority of the genes that were cell-type enriched at the slug stage were first expressed as the prespore and prestalk cells sorted out in aggregates, some were found to be expressed earlier before the cells had even aggregated. These early genes may have been initially expressed in all cells and then preferentially turned over in one or the other cell type. Alternatively, cell type divergence may start soon after the initiation of development.

scholarly journals Trans- and cis-acting effects of Firre on epigenetic features of the inactive X chromosome

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
He Fang ◽  
Giancarlo Bonora ◽  
Jordan P. Lewandowski ◽  
Jitendra Thakur ◽  
Galina N. Filippova ◽  
...  
Keyword(s):  
X Chromosome ◽  
3D Structure ◽  
Ectopic Expression ◽  
Ctcf Binding ◽  
Cell Type ◽  
Cis Acting ◽  
Gene Dysregulation ◽  
Inactive X Chromosome ◽  
Cell Type Specific ◽  
Polycomb Repressive Complexes

AbstractFirre encodes a lncRNA involved in nuclear organization. Here, we show that Firre RNA expressed from the active X chromosome maintains histone H3K27me3 enrichment on the inactive X chromosome (Xi) in somatic cells. This trans-acting effect involves SUZ12, reflecting interactions between Firre RNA and components of the Polycomb repressive complexes. Without Firre RNA, H3K27me3 decreases on the Xi and the Xi-perinucleolar location is disrupted, possibly due to decreased CTCF binding on the Xi. We also observe widespread gene dysregulation, but not on the Xi. These effects are measurably rescued by ectopic expression of mouse or human Firre/FIRRE transgenes, supporting conserved trans-acting roles. We also find that the compact 3D structure of the Xi partly depends on the Firre locus and its RNA. In common lymphoid progenitors and T-cells Firre exerts a cis-acting effect on maintenance of H3K27me3 in a 26 Mb region around the locus, demonstrating cell type-specific trans- and cis-acting roles of this lncRNA.

scholarly journals Ectopic synthesis of epidermal cytokeratins in pancreatic islet cells of transgenic mice interferes with cytoskeletal order and insulin production.

1993 ◽  
Vol 120 (3) ◽  
pp. 743-755 ◽  
Author(s):  
M Blessing ◽  
U Rüther ◽  
W W Franke
Keyword(s):  
Transgenic Mice ◽  
Beta Cells ◽  
Cell Function ◽  
Cultured Cells ◽  
Ectopic Expression ◽  
Epidermal Keratinocytes ◽  
Cell Type ◽  
Cell Functions ◽  
Insulin Promoter ◽  
Cell Type Specific

The members of the multigene family of intermediate filament (IF) proteins are expressed in various combinations and amounts that are specific for a given pathway or state of differentiation. Previous experiments in which the cell type-specific IF cytoskeleton was altered by introducing foreign IF proteins into cultured cells or certain tissues of transgenic animals have shown a remarkable tolerance, without detectable interference with cell functions. To examine the importance of the cell type-specific cytokeratin (CK) IF pattern, we have studied the ectopic expression of CK genes in different epithelia of transgenic mice. Here we report changes observed in the beta cells of pancreatic islets expressing the genes for human epidermal CKs 1 and/or 10 brought under control of the rat insulin promoter. Both genes were efficiently expressed, resulting in the appearance of numerous and massive bundles of aggregated IFs, resembling those of epidermal keratinocytes. While the synthesis of epidermal CK 10 was readily accommodated and compatible with cell function, mice expressing CK 1 in their beta cells, alone or in combination with CK 10, developed a special form of diabetes characterized by a drastic reduction of insulin-secretory vesicles and of insulin-and CK 1-producing cells. In many CK 1-producing cells, accumulations of fibrous or granular material containing CK 1 were also seen in the nucleus. This demonstration of functional importance of the specific CK-complement in an epithelial cell indicates a contribution of cell type-specific factors to cytoplasmic IF compartmentalization and that the specific CK complement can be crucial for functions and longevity of a given kind of epithelium.

scholarly journals Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis–infected lungs

PLoS Biology ◽  
2020 ◽  
Vol 18 (12) ◽  
pp. e3000879
Author(s):  
Antony Fearns ◽  
Daniel J. Greenwood ◽  
Angela Rodgers ◽  
Haibo Jiang ◽  
Maximiliano G. Gutierrez
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Infected Tissue ◽  
Cell Type ◽  
Lung Lesions ◽  
Specific Accumulation ◽  
Cell Type Specific ◽  
Ion Microscopy ◽  
Foamy Macrophages ◽  
Intracellular Fate

Correlative light, electron, and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, we developed correlative light, electron, and ion microscopy in tissue (CLEIMiT) and used it to identify the cell type–specific accumulation of an antibiotic in lung lesions of mice infected with Mycobacterium tuberculosis. Using CLEIMiT, we found that the anti-tuberculosis (TB) drug bedaquiline (BDQ) is localised not only in foamy macrophages in the lungs during infection but also accumulate in polymorphonuclear (PMN) cells.

scholarly journals C/EBPβ Induces Chromatin Opening at a Cell-Type-Specific Enhancer

2008 ◽  
Vol 28 (6) ◽  
pp. 2102-2112 ◽  
Author(s):  
Annette Plachetka ◽  
Olesya Chayka ◽  
Carola Wilczek ◽  
Svitlana Melnik ◽  
Constanze Bonifer ◽  
...  
Keyword(s):  
Protein C ◽  
Ectopic Expression ◽  
Cell Type ◽  
Target Region ◽  
Enhancer Binding Protein ◽  
Hypersensitive Sites ◽  
A Cell ◽  
Cell Type Specific ◽  
Myelomonocytic Cells ◽  
Chromatin Opening

ABSTRACT We have used the chicken mim-1 gene as a model to study the mechanisms by which transcription factors gain initial access to their target sites in compacted chromatin. The expression of mim-1 is restricted to the myelomonocytic lineage of the hematopoietic system where it is regulated synergistically by the Myb and CCAAT/enhancer binding protein (C/EBP) factors. Myb and C/EBPβ cooperate at two distinct cis elements of mim-1, the promoter and a cell-type-specific enhancer, both of which are associated with DNase I hypersensitive sites in myelomonocytic cells but not in mim-1-nonexpressing cells. Previous work has shown that ectopic expression of Myb and C/EBPβ activates the endogenous mim-1 gene in a nonhematopoietic cell type (fibroblasts), where the gene is normally completely silent. Here, we investigated the molecular details of this finding and show that the activation of mim-1 occurs by two independent mechanisms. In the absence of Myb, C/EBPβ triggers the initial steps of chromatin opening at the mim-1 enhancer without inducing transcription of the gene. mim-1 transcription occurs only in the presence of Myb and is associated with chromatin opening at the promoter. Our work identifies a novel function for C/EBPβ in the initial steps of a localized chromatin opening at a specific, physiologically relevant target region.

scholarly journals Samd7 is a cell type-specific PRC1 component essential for establishing retinal rod photoreceptor identity

2017 ◽  
Vol 114 (39) ◽  
pp. E8264-E8273 ◽  
Author(s):  
Yoshihiro Omori ◽  
Shun Kubo ◽  
Tetsuo Kon ◽  
Mayu Furuhashi ◽  
Hirotaka Narita ◽  
...  
Keyword(s):  
Gene Expression ◽  
Photoreceptor Cell ◽  
Ectopic Expression ◽  
Neuronal Cell ◽  
Photoreceptor Cells ◽  
Rod Photoreceptor ◽  
Cell Type ◽  
A Cell ◽  
Cell Type Specific ◽  
Null Mutant Mice

Precise transcriptional regulation controlled by a transcription factor network is known to be crucial for establishing correct neuronal cell identities and functions in the CNS. In the retina, the expression of various cone and rod photoreceptor cell genes is regulated by multiple transcription factors; however, the role of epigenetic regulation in photoreceptor cell gene expression has been poorly understood. Here, we found that Samd7, a rod-enriched sterile alpha domain (SAM) domain protein, is essential for silencing nonrod gene expression through H3K27me3 regulation in rod photoreceptor cells. Samd7-null mutant mice showed ectopic expression of nonrod genes including S-opsin in rod photoreceptor cells and rod photoreceptor cell dysfunction. Samd7 physically interacts with Polyhomeotic homologs (Phc proteins), components of the Polycomb repressive complex 1 (PRC1), and colocalizes with Phc2 and Ring1B in Polycomb bodies. ChIP assays showed a significant decrease of H3K27me3 in the genes up-regulated in the Samd7-deficient retina, showing that Samd7 deficiency causes the derepression of nonrod gene expression in rod photoreceptor cells. The current study suggests that Samd7 is a cell type-specific PRC1 component epigenetically defining rod photoreceptor cell identity.

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