scholarly journals Pattern of Deposition of Cell Wall Polysaccharides and Transcript Abundance of Related Cell Wall Synthesis Genes during Differentiation in Barley Endosperm

2012 ◽  
Vol 159 (2) ◽  
pp. 655-670 ◽  
Author(s):  
Sarah M. Wilson ◽  
Rachel A. Burton ◽  
Helen M. Collins ◽  
Monika S. Doblin ◽  
Filomena A. Pettolino ◽  
...  
2013 ◽  
Vol 289 (3) ◽  
pp. 1243-1256 ◽  
Author(s):  
Mark J. Lee ◽  
Fabrice N. Gravelat ◽  
Robert P. Cerone ◽  
Stefanie D. Baptista ◽  
Paolo V. Campoli ◽  
...  

The cell wall of Aspergillus fumigatus contains two galactose-containing polysaccharides, galactomannan and galactosaminogalactan, whose biosynthetic pathways are not well understood. The A. fumigatus genome contains three genes encoding putative UDP-glucose 4-epimerases, uge3, uge4, and uge5. We undertook this study to elucidate the function of these epimerases. We found that uge4 is minimally expressed and is not required for the synthesis of galactose-containing exopolysaccharides or galactose metabolism. Uge5 is the dominant UDP-glucose 4-epimerase in A. fumigatus and is essential for normal growth in galactose-based medium. Uge5 is required for synthesis of the galactofuranose (Galf) component of galactomannan and contributes galactose to the synthesis of galactosaminogalactan. Uge3 can mediate production of both UDP-galactose and UDP-N-acetylgalactosamine (GalNAc) and is required for the production of galactosaminogalactan but not galactomannan. In the absence of Uge5, Uge3 activity is sufficient for growth on galactose and the synthesis of galactosaminogalactan containing lower levels of galactose but not the synthesis of Galf. A double deletion of uge5 and uge3 blocked growth on galactose and synthesis of both Galf and galactosaminogalactan. This study is the first survey of glucose epimerases in A. fumigatus and contributes to our understanding of the role of these enzymes in metabolism and cell wall synthesis.


2020 ◽  
Author(s):  
Song Chen ◽  
Xiyang Zhao ◽  
Su Chen

Abstract Cellulose is an essential structural component of the plant cell wall and is an important resource for the production of paper, textiles, bioplastics and other biomaterials. The synthesis of cellulose is among the most important but poorly understood biochemical processes, which is precisely regulated by internal and external cues. Here we identified 46 gene models in 7 gene families which encoding cellulose synthase and related enzymes of Betula pendula , and the transcript abundance of these genes in xylem, root, leaf and flower tissues also be determined. Based on these RNA-seq data, we have identified 8 genes that most likely participate in cell wall synthesis. In parallel, a gene co-expression network was also constructed based on transcriptome sequencing.


2020 ◽  
Author(s):  
Song Chen ◽  
Xiyang Zhao ◽  
Su Chen

AbstractCellulose is an essential structural component of the plant cell wall and is an important resource for the production of paper, textiles, bioplastics and other biomaterials. The synthesis of cellulose is among the most important but poorly understood biochemical processes, which is precisely regulated by internal and external cues. Here we identified 46 gene models in 7 gene families which encoding cellulose synthase and related enzymes of Betula pendula, and the transcript abundance of these genes in xylem, root, leaf and flower tissues also be determined. Based on these RNA-seq data, we have identified 8 genes that most likely participate in cell wall synthesis. In parallel, a gene co-expression network was also constructed based on transcriptome sequencing.FundingThis work was supported by the National Natural Science Foundation of China, grant number 31870659, The Fundamental Research Funds for the Central Universities, grant number 2572019CG08 funded this research and Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team).Conflicts of interest / Competing interestsThe authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.Consent to participateNot applicable.Consent for publicationNot applicable.Availability of data and materialAll data generated or analyzed during this study are included in this published article.Code availabilityNot applicable.Authors’ contributionsAll authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Song Chen. Conceived and supervised were performed by Xiyang Zhao and Su Chen.


Crop Science ◽  
2003 ◽  
Vol 43 (2) ◽  
pp. 571 ◽  
Author(s):  
S. K. Stombaugh ◽  
J. H. Orf ◽  
H. G. Jung ◽  
D. A. Somers

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