scholarly journals Assembly of the Light-Harvesting Chlorophyll Antenna in the Green Alga Chlamydomonas reinhardtii Requires Expression of the TLA2-CpFTSY Gene

2011 ◽  
Vol 158 (2) ◽  
pp. 930-945 ◽  
Author(s):  
Henning Kirst ◽  
Jose Gines García-Cerdán ◽  
Andreas Zurbriggen ◽  
Anastasios Melis
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
Light Harvesting

Light-Harvesting Strategy during CO2-Dependent Photosynthesis in the Green Alga Chlamydomonas reinhardtii

2018 ◽  
Vol 9 (5) ◽  
pp. 1028-1033 ◽  
Author(s):  
Yoshifumi Ueno ◽  
Ginga Shimakawa ◽  
Chikahiro Miyake ◽  
Seiji Akimoto
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
Light Harvesting

Regulation of Light Harvesting in the Green Alga Chlamydomonas reinhardtii: The C-Terminus of LHCSR Is the Knob of a Dimmer Switch

2013 ◽  
Vol 135 (49) ◽  
pp. 18339-18342 ◽  
Author(s):  
Nicoletta Liguori ◽  
Laura M. Roy ◽  
Milena Opacic ◽  
Grégory Durand ◽  
Roberta Croce
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
Light Harvesting ◽  
C Terminus

Assembly Apparatus of Light-Harvesting Complexes; Identification of Alb3.1-cpSRP-LHCP Complexes in the Green Alga Chlamydomonas reinhardtii

2021 ◽  
Author(s):  
Mithun Kumar Rathod ◽  
Nellaipalli Sreedhar ◽  
Shin-ichiro Ozawa ◽  
Hiroshi Kuroda ◽  
Natsumi Kodama ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
Light Harvesting ◽  
Affinity Purification ◽  
Signal Recognition Particle ◽  
Thylakoid Membranes ◽  
Signal Recognition ◽  
Light Harvesting Complexes ◽  
C Terminus ◽  
Unicellular Green Alga

Abstract The unicellular green alga, Chlamydomonas reinhardtii, contains many light-harvesting complexes (LHCs) associating chlorophylls a/b and carotenoids; the major light-harvesting complexes, LHCIIs (types I, II, III, and IV), and minor light-harvesting complexes, CP26 and CP29, for photosystem II, as well as nine light-harvesting complexes, LHCIs (LHCA1-9), for photosystem I. A pale green mutant BF4 exhibited impaired accumulation of LHCs due to deficiency in Alb3.1 gene which encodes the insertase involved in insertion, folding and assembly of LHC proteins in the thylakoid membranes. To elucidate the molecular mechanism by which ALB3.1 assists LHC assembly, we complemented BF4 to express ALB3.1 fused with no, single, or triple HA tag at its C-terminus (cAlb3.1, cAlb3.1-HA, or cAlb3.1-3HA). The resulting complemented strains accumulated most LHC proteins comparable to wild-type levels. The affinity purification of Alb3.1-HA and Alb3.1-3HA preparations showed that ALB3.1 interacts with cpSRP43 and cpSRP54 proteins of chloroplast signal recognition particle cpSRP and several LHC proteins; two major LHCII proteins (types I and III), two minor LHCII proteins (CP26 and CP29), and eight LHCI proteins (LHCA1, 2, 3, 4, 5, 6, 8, and 9). Pulse-chase labeling experiments revealed that the newly synthesized major LHCII proteins were transiently bound to the Alb3.1 complex. We propose that Alb3.1 interacts with cpSRP43 and cpSRP54 to form an assembly apparatus for most LHCs in the thylakoid membranes. Interestingly, PSI proteins were also detected in the Alb3.1 preparations, suggesting that the integration of LHCIs to a PSI core complex to form a PSI-LHCI subcomplex occurs before assembled LHCIs dissociate from the Alb3.1-cpSRP complex.

scholarly journals Transcriptome for Photobiological Hydrogen Production Induced by Sulfur Deprivation in the Green Alga Chlamydomonas reinhardtii

2008 ◽  
Vol 7 (11) ◽  
pp. 1965-1979 ◽  
Author(s):  
Anh Vu Nguyen ◽  
Skye R. Thomas-Hall ◽  
Alizée Malnoë ◽  
Matthew Timmins ◽  
Jan H. Mussgnug ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
Light Harvesting ◽  
Expression Profiles ◽  
Transcriptional Analysis ◽  
Sulfur Deprivation ◽  
Light Harvesting Complex ◽  
Sulfur Starvation ◽  
Reverse Transcription Pcr

ABSTRACT Photobiological hydrogen production using microalgae is being developed into a promising clean fuel stream for the future. In this study, microarray analyses were used to obtain global expression profiles of mRNA abundance in the green alga Chlamydomonas reinhardtii at different time points before the onset and during the course of sulfur-depleted hydrogen production. These studies were followed by real-time quantitative reverse transcription-PCR and protein analyses. The present work provides new insights into photosynthesis, sulfur acquisition strategies, and carbon metabolism-related gene expression during sulfur-induced hydrogen production. A general trend toward repression of transcripts encoding photosynthetic genes was observed. In contrast to all other LHCBM genes, the abundance of the LHCBM9 transcript (encoding a major light-harvesting polypeptide) and its protein was strongly elevated throughout the experiment. This suggests a major remodeling of the photosystem II light-harvesting complex as well as an important function of LHCBM9 under sulfur starvation and photobiological hydrogen production. This paper presents the first global transcriptional analysis of C. reinhardtii before, during, and after photobiological hydrogen production under sulfur deprivation.

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