scholarly journals PERSISTENT TAPETAL CELL1 Encodes a PHD-Finger Protein That Is Required for Tapetal Cell Death and Pollen Development in Rice

2011 ◽  
Vol 156 (2) ◽  
pp. 615-630 ◽  
Author(s):  
Hui Li ◽  
Zheng Yuan ◽  
Gema Vizcay-Barrena ◽  
Caiyun Yang ◽  
Wanqi Liang ◽  
...  
Keyword(s):  
Cell Death ◽  
Pollen Development ◽  
Tapetal Cell ◽  
Phd Finger ◽  
Finger Protein

scholarly journals The Cysteine Protease CEP1, a Key Executor Involved in Tapetal Programmed Cell Death, Regulates Pollen Development in Arabidopsis

2014 ◽  
Vol 26 (7) ◽  
pp. 2939-2961 ◽  
Author(s):  
Dandan Zhang ◽  
Di Liu ◽  
Xiaomeng Lv ◽  
Ying Wang ◽  
Zhili Xun ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Cysteine Protease ◽  
Pollen Development

scholarly journals PHD finger protein 1 (PHF1) is a novel reader for histone H4R3 symmetric dimethylation and coordinates with PRMT5–WDR77/CRL4B complex to promote tumorigenesis

2018 ◽  
Vol 46 (13) ◽  
pp. 6608-6626 ◽  
Author(s):  
Ruiqiong Liu ◽  
Jie Gao ◽  
Yang Yang ◽  
Rongfang Qiu ◽  
Yu Zheng ◽  
...  
Keyword(s):  
Phd Finger ◽  
Finger Protein

scholarly journals Epigenetic reader complexes of the human malaria parasite, Plasmodium falciparum

2019 ◽  
Vol 47 (22) ◽  
pp. 11574-11588 ◽  
Author(s):  
Wieteke Anna Maria Hoeijmakers ◽  
Jun Miao ◽  
Sabine Schmidt ◽  
Christa Geeke Toenhake ◽  
Sony Shrestha ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Protein Complexes ◽  
Human Malaria Parasite ◽  
Phd Finger ◽  
Finger Protein ◽  
Binding Preference ◽  
Parasite Plasmodium ◽  
Parasite Plasmodium Falciparum ◽  
Eukaryotic Organisms ◽  
Interaction Proteomics

Abstract Epigenetic regulatory mechanisms are central to the development and survival of all eukaryotic organisms. These mechanisms critically depend on the marking of chromatin domains with distinctive histone tail modifications (PTMs) and their recognition by effector protein complexes. Here we used quantitative proteomic approaches to unveil interactions between PTMs and associated reader protein complexes of Plasmodium falciparum, a unicellular parasite causing malaria. Histone peptide pull-downs with the most prominent and/or parasite-specific PTMs revealed the binding preference for 14 putative and novel reader proteins. Amongst others, they highlighted the acetylation-level-dependent recruitment of the BDP1/BDP2 complex and identified an PhD-finger protein (PHD 1, PF3D7_1008100) that could mediate a cross-talk between H3K4me2/3 and H3K9ac marks. Tagging and interaction proteomics of 12 identified proteins unveiled the composition of 5 major epigenetic complexes, including the elusive TBP-associated-factor complex as well as two distinct GCN5/ADA2 complexes. Furthermore, it has highlighted a remarkable degree of interaction between these five (sub)complexes. Collectively, this study provides an extensive inventory of PTM-reader interactions and composition of epigenetic complexes. It will not only fuel further explorations of gene regulation amongst ancient eukaryotes, but also provides a stepping stone for exploration of PTM-reader interactions for antimalarial drug development.

scholarly journals The KAP1 Corepressor Functions To Coordinate the Assembly of De Novo HP1-Demarcated Microenvironments of Heterochromatin Required for KRAB Zinc Finger Protein-Mediated Transcriptional Repression

2006 ◽  
Vol 26 (22) ◽  
pp. 8623-8638 ◽  
Author(s):  
Smitha P. Sripathy ◽  
Jessica Stevens ◽  
David C. Schultz
Keyword(s):  
Rna Polymerase Ii ◽  
Zinc Finger ◽  
Histone Modifications ◽  
Dna Sequences ◽  
Transcriptional Repression ◽  
Zinc Finger Protein ◽  
De Novo ◽  
Regulatory Sequences ◽  
Phd Finger ◽  
Finger Protein

ABSTRACT KAP1/TIF1β is proposed to be a universal corepressor protein for the KRAB zinc finger protein (KRAB-zfp) superfamily of transcriptional repressors. To characterize the role of KAP1 and KAP1-interacting proteins in transcriptional repression, we investigated the regulation of stably integrated reporter transgenes by hormone-responsive KRAB and KAP1 repressor proteins. Here, we demonstrate that depletion of endogenous KAP1 levels by small interfering RNA (siRNA) significantly inhibited KRAB-mediated transcriptional repression of a chromatin template. Similarly, reduction in cellular levels of HP1α/β/γ and SETDB1 by siRNA attenuated KRAB-KAP1 repression. We also found that direct tethering of KAP1 to DNA was sufficient to repress transcription of an integrated transgene. This activity is absolutely dependent upon the interaction of KAP1 with HP1 and on an intact PHD finger and bromodomain of KAP1, suggesting that these domains function cooperatively in transcriptional corepression. The achievement of the repressed state by wild-type KAP1 involves decreased recruitment of RNA polymerase II, reduced levels of histone H3 K9 acetylation and H3K4 methylation, an increase in histone occupancy, enrichment of trimethyl histone H3K9, H3K36, and histone H4K20, and HP1 deposition at proximal regulatory sequences of the transgene. A KAP1 protein containing a mutation of the HP1 binding domain failed to induce any change in the histone modifications associated with DNA sequences of the transgene, implying that HP1-directed nuclear compartmentalization is required for transcriptional repression by the KRAB/KAP1 repression complex. The combination of these data suggests that KAP1 functions to coordinate activities that dynamically regulate changes in histone modifications and deposition of HP1 to establish a de novo microenvironment of heterochromatin, which is required for repression of gene transcription by KRAB-zfps.

scholarly journals Overexpression of Two CCCH-type Zinc-Finger Protein Genes Leads to Pollen Abortion in Brassica campestris ssp. chinensis

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1287
Author(s):  
Liai Xu ◽  
Tingting Liu ◽  
Xingpeng Xiong ◽  
Weimiao Liu ◽  
Youjian Yu ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Zinc Finger ◽  
Pollen Development ◽  
Brassica Campestris ◽  
Zinc Finger Protein ◽  
Function Analysis ◽  
Pollen Grains ◽  
Functional Redundancy ◽  
Pollen Abortion ◽  
Finger Protein

The pollen grains produced by flowering plants are vital for sexual reproduction. Previous studies have shown that two CCCH-type zinc-finger protein genes in Brassica campestris, BcMF30a and BcMF30c, are involved in pollen development. Due to their possible functional redundancy, gain-of-function analysis is helpful to reveal their respective biological functions. Here, we found that the phenotypes of BcMF30a and BcMF30c overexpression transgenic plants driven by their native promoters were similar, suggesting their functional redundancy. The results showed that the vegetative growth was not affected in both transgenic plants, but male fertility was reduced. Further analysis found that the abortion of transgenic pollen was caused by the degradation of pollen contents from the late uninucleate microspore stage. Subcellular localization analysis demonstrated that BcMF30a and BcMF30c could localize in cytoplasmic foci. Combined with the studies of other CCCH-type genes, we speculated that the overexpression of these genes can induce the continuous assembly of abnormal cytoplasmic foci, thus resulting in defective plant growth and development, which, in this study, led to pollen abortion. Both the overexpression and knockout of BcMF30a and BcMF30c lead to abnormal pollen development, indicating that the appropriate expression levels of these two genes are critical for the maintenance of normal pollen development.

scholarly journals Yin Yang 1 is required for PHD finger protein 20-mediated myogenic differentiation in vitro and in vivo

2020 ◽  
Vol 27 (12) ◽  
pp. 3321-3336
Author(s):  
Hyunji Lee ◽  
Youngeun Hong ◽  
Gyeyeong Kong ◽  
Dong Hoon Lee ◽  
Minhee Kim ◽  
...  
Keyword(s):  
Myogenic Differentiation ◽  
Phd Finger ◽  
Yin Yang 1 ◽  
Finger Protein ◽  
Yin Yang

scholarly journals Impact of Autophagy on Gene Expression and Tapetal Programmed Cell Death During Pollen Development in Rice

2020 ◽  
Vol 11 ◽  
Author(s):  
Shigeru Hanamata ◽  
Jumpei Sawada ◽  
Seijiro Ono ◽  
Kazunori Ogawa ◽  
Togo Fukunaga ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Pollen Development

scholarly journals βVPE is involved in tapetal degradation and pollen development by activating proprotease maturation in Arabidopsis thaliana

2019 ◽  
Vol 71 (6) ◽  
pp. 1943-1955 ◽  
Author(s):  
Ziyi Cheng ◽  
Xiaorui Guo ◽  
Jiaxue Zhang ◽  
Yadi Liu ◽  
Bing Wang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Development ◽  
Tapetal Cell ◽  
Cysteine Proteases ◽  
Anther Development ◽  
Mature Protein ◽  
Processing Enzyme ◽  
Mature Enzyme ◽  
Mature Protease

Abstract Vacuolar processing enzyme (VPE) is responsible for the maturation and activation of vacuolar proteins in plants. We found that βVPE was involved in tapetal degradation and pollen development by transforming proproteases into mature protease in Arabidopsis thaliana. βVPE was expressed specifically in the tapetum from stages 5 to 8 of anther development. The βVPE protein first appeared as a proenzyme and was transformed into the mature enzyme before stages 7–8. The recombinant βVPE protein self-cleaved and transformed into a 27 kDa mature protein at pH 5.2. The mature βVPE protein could induce the maturation of CEP1 in vitro. βvpe mutants exhibited delayed vacuolar degradation and decreased pollen fertility. The maturation of CEP1, RD19A, and RD19C was seriously inhibited in βvpe mutants. Our results indicate that βVPE is a crucial processing enzyme that directly participates in the maturation of cysteine proteases before vacuolar degradation, and is indirectly involved in pollen development and tapetal cell degradation.

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