scholarly journals Suborganellar Localization and Molecular Characterization of Nonproteolytic Degraded Leukoplast Pyruvate Kinase from Developing Castor Oil Seeds

1995 ◽  
Vol 109 (4) ◽  
pp. 1461-1469 ◽  
Author(s):  
F. B. Negm ◽  
F. A. Cornel ◽  
W. C. Plaxton
Keyword(s):  
Molecular Characterization ◽  
Pyruvate Kinase ◽  
Castor Oil ◽  
Oil Seeds

scholarly journals Biochemical and Molecular Characterization of RcSUS1, a Cytosolic Sucrose Synthase Phosphorylatedin Vivoat Serine 11 in Developing Castor Oil Seeds

2014 ◽  
Vol 289 (48) ◽  
pp. 33412-33424 ◽  
Author(s):  
Eric T. Fedosejevs ◽  
Sheng Ying ◽  
Joonho Park ◽  
Erin M. Anderson ◽  
Robert T. Mullen ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Sucrose Synthase ◽  
Castor Oil ◽  
Oil Seeds

scholarly journals Kinetic and regulatory properties of cytosolic pyruvate kinase from germinating castor oil seeds

1991 ◽  
Vol 279 (2) ◽  
pp. 495-501 ◽  
Author(s):  
F E Podestá ◽  
W C Plaxton
Keyword(s):  
Pyruvate Kinase ◽  
Castor Oil ◽  
Mixed Type ◽  
Competitive Inhibition ◽  
Ricinus Communis ◽  
Cytosolic Ph ◽  
Oil Seeds ◽  
Saturation Kinetics ◽  
Regulatory Properties

The kinetic and regulatory properties of cytosolic pyruvate kinase (PKc) isolated from endosperm of germinating castor oil seeds (Ricinus communis L.) have been studied. Optimal efficiency in substrate utilization (in terms of Vmax/Km for phosphoenolpyruvate or ADP) occurred between pH 6.7 and 7.4. Enzyme activity was absolutely dependent on the presence of a bivalent and a univalent metal cation, with Mg2+ and K+ fulfilling this requirement. Mg2+ binding showed positive and negative co-operativity at pH 6.5 (h = 1.6) and pH 7.2 (h = 0.69) respectively. Hyperbolic saturation kinetics were observed with phosphoenolpyruvate (PEP) and K+, whereas ADP acted as a mixed-type inhibitor over 1 mM. Glycerol (10%, v/v) increased the S0.5(ADP) 2.3-fold and altered the pattern of nucleotide binding from hyperbolic (h = 1.0) to sigmoidal (h = 1.79) without modifying PEP saturation kinetics. No activators were identified. ATP, AMP, isocitrate, 2-oxoglutarate, malate, 2-phosphoglycerate, 2,3-bisphosphoglycerate, 3-phosphoglycerate, glycerol 3-phosphate and phosphoglycolate were the most effective inhibitors. These metabolites yielded additive inhibition when tested in pairs. ATP and 3-phosphoglycerate were mixed-type inhibitors with respect to PEP, whereas competitive inhibition was observed for other inhibitors. Inhibition by malate, 2-oxoglutarate, phosphorylated triose sugars or phosphoglycolate was far more pronounced at pH 7.2 than at pH 6.5. Although 32P-labelling studies revealed that extensive phosphorylation in vivo of soluble endosperm proteins occurred between days 3 and 5 of seed germination, no alteration in the 32P-labelling pattern of 5-day-germinated endosperm was observed after 30 min of anaerobiosis. Moreover, no evidence was obtained that PKc was a phosphoprotein in aerobic or anoxic endosperms. It is proposed that endosperm PKc activity of germinating castor seeds is enhanced after anaerobiosis through concerted decreases in ATP levels, cytosolic pH and concentrations of several key inhibitors.

Six Children with Pyruvate Kinase Deficiency from One Small Town: Molecular Characterization of the PK-LR Gene

2011 ◽  
Vol 159 (4) ◽  
pp. 695-697 ◽  
Author(s):  
Robert D. Christensen ◽  
Hassan M. Yaish ◽  
Charlotte B. Johnson ◽  
Paola Bianchi ◽  
Alberto Zanella
Keyword(s):  
Molecular Characterization ◽  
Pyruvate Kinase ◽  
Small Town ◽  
Pyruvate Kinase Deficiency

Molecular characterization of plastid pyruvate kinase from castor and tobacco

1995 ◽  
Vol 27 (1) ◽  
pp. 79-89 ◽  
Author(s):  
Stephen Blakeley ◽  
Sylvia Gottlob-McHugh ◽  
Jiangxin Wan ◽  
Linda Crews ◽  
Brian Miki ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Pyruvate Kinase

scholarly journals Leucoplast Pyruvate Kinase from Developing Castor Oil Seeds

1991 ◽  
Vol 97 (4) ◽  
pp. 1334-1338 ◽  
Author(s):  
William C. Plaxton
Keyword(s):  
Pyruvate Kinase ◽  
Castor Oil ◽  
Oil Seeds

Molecular characterization of the PK-LR gene in pyruvate kinase deficient Spanish patients

1998 ◽  
Vol 103 (2) ◽  
pp. 377-382 ◽  
Author(s):  
Zarza ◽  
Alvarez ◽  
Pujades ◽  
Nomdedeu ◽  
Carrera ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Pyruvate Kinase
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