scholarly journals The Regulation of Carbonic Anhydrase and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activase by Light and CO2 in Chlamydomonas reinhardtii

1995 ◽  
Vol 109 (3) ◽  
pp. 937-944 ◽  
Author(s):  
M. Rawat ◽  
J. V. Moroney
Keyword(s):  
Carbonic Anhydrase ◽  
Chlamydomonas Reinhardtii ◽  
Bisphosphate Carboxylase

scholarly journals Identification of Extracellular Carbonic Anhydrase of Chlamydomonas reinhardtii

1984 ◽  
Vol 76 (2) ◽  
pp. 472-477 ◽  
Author(s):  
John R. Coleman ◽  
Joseph A. Berry ◽  
Robert K. Togasaki ◽  
Arthur R. Grossman
Keyword(s):  
Carbonic Anhydrase ◽  
Chlamydomonas Reinhardtii

The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation

1994 ◽  
Vol 14 (6) ◽  
pp. 4011-4019
Author(s):  
J A Nelson ◽  
P B Savereide ◽  
P A Lefebvre
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Selectable Marker ◽  
Marker Gene ◽  
Small Subunit ◽  
Direct Selection ◽  
Nuclear Transformation ◽  
Ribulose Bisphosphate Carboxylase ◽  
Bisphosphate Carboxylase ◽  
Dominant Selectable Marker ◽  
Cry1 Gene

We have cloned and sequenced the CRY1 gene, encoding ribosomal protein S14 in Chlamydomonas reinhardtii, and found that it is highly similar to S14/rp59 proteins from other organisms, including mammals, Drosophila melanogaster, and Saccharomyces cerevisiae. We isolated a mutant strain resistant to the eukaryotic translational inhibitors cryptopleurine and emetine in which the resistance was due to a missense mutation (CRY1-1) in the CRY1 gene; resistance was dominant in heterozygous stable diploids. Cotransformation experiments using the CRY1-1 gene and the gene for nitrate reductase (NIT1) produced a low level of resistance to cryptopleurine and emetine. Resistance levels were increased when the CRY1-1 gene was placed under the control of a constitutive promoter from the ribulose bisphosphate carboxylase/oxygenase small subunit 2 (RBCS2) gene. We also found that the 5' untranslated region of the CRY1 gene was required for expression of the CRY1-1 transgene. Direct selection of emetine-resistant transformants was possible when transformed cells were first induced to differentiate into gametes by nitrogen starvation and then allowed to dedifferentiate back to vegetative cells before emetine selection was applied. With this transformation protocol, the RBCS2/CRY1-1 dominant selectable marker gene is a powerful tool for many molecular genetic applications in C. reinhardtii.

scholarly journals Identification and functional role of the carbonic anhydrase Cah3 in thylakoid membranes of pyrenoid of Chlamydomonas reinhardtii

2012 ◽  
Vol 1817 (8) ◽  
pp. 1248-1255 ◽  
Author(s):  
Maria A. Sinetova ◽  
Elena V. Kupriyanova ◽  
Alexandra G. Markelova ◽  
Suleyman I. Allakhverdiev ◽  
Natalia A. Pronina
Keyword(s):  
Carbonic Anhydrase ◽  
Chlamydomonas Reinhardtii ◽  
Functional Role ◽  
Thylakoid Membranes

scholarly journals The Novel Myb Transcription Factor LCR1 Regulates the CO2-Responsive Gene Cah1, Encoding a Periplasmic Carbonic Anhydrase in Chlamydomonas reinhardtii

2004 ◽  
Vol 16 (6) ◽  
pp. 1466-1477 ◽  
Author(s):  
Satoshi Yoshioka ◽  
Fumiya Taniguchi ◽  
Kenji Miura ◽  
Takeshi Inoue ◽  
Takashi Yamano ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Carbonic Anhydrase ◽  
Chlamydomonas Reinhardtii ◽  
Myb Transcription Factor ◽  
The Novel ◽  
Responsive Gene
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