scholarly journals Increasing Sucrose Uptake Capacity of Wheat Grains Stimulates Storage Protein Synthesis

2009 ◽  
Vol 152 (2) ◽  
pp. 698-710 ◽  
Author(s):  
Nicola Weichert ◽  
Isolde Saalbach ◽  
Heiko Weichert ◽  
Stefan Kohl ◽  
Alexander Erban ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Storage Protein ◽  
Sucrose Uptake ◽  
Uptake Capacity ◽  
Wheat Grains

STUDY OF MAIZE STORAGE PROTEIN SYNTHESIS IN CELL-FREE SYSTEM

1981 ◽  
Vol 9 (2) ◽  
pp. 203P-203P
Author(s):  
M. Denic ◽  
K. Konstantinov ◽  
V. Popovic
Keyword(s):  
Protein Synthesis ◽  
Storage Protein ◽  
Free System ◽  
Cell Free System

scholarly journals Storage Protein Synthesis in Maize

1976 ◽  
Vol 57 (5) ◽  
pp. 740-745 ◽  
Author(s):  
Brian A. Larkins ◽  
Charles E. Bracker ◽  
C. Y. Tsai
Keyword(s):  
Protein Synthesis ◽  
Storage Protein

scholarly journals Storage Protein Synthesis in Maize

1977 ◽  
Vol 59 (4) ◽  
pp. 525-529 ◽  
Author(s):  
Richard A. Jones ◽  
Brian A. Larkins ◽  
C. Y. Tsai
Keyword(s):  
Protein Synthesis ◽  
Storage Protein

Increased capacity for sucrose uptake leads to earlier onset of protein accumulation in developing pea seeds

2005 ◽  
Vol 32 (11) ◽  
pp. 997 ◽  
Author(s):  
Elke G. Rosche ◽  
Daniel Blackmore ◽  
Christina E. Offler ◽  
John W. Patrick
Keyword(s):  
Storage Protein ◽  
Protein Biosynthesis ◽  
Protein Bodies ◽  
Protein Accumulation ◽  
Sucrose Uptake ◽  
Wild Type ◽  
Protein Levels ◽  
Vicilin Gene ◽  
And Storage

Pea (Pisum sativum L.) cotyledons, overexpressing a potato sucrose transporter (StSUT1), were used to explore the hypothesis that sucrose stimulates the onset of storage protein biosynthesis. The study focused on the transition between pre-storage and storage phases of seed development. During this period supply of sucrose and hexose to transgenic cotyledons was unaffected by StSUT1 expression. However, protoplasmic levels of sucrose but not hexoses were elevated in transgenic cotyledons. Total protein levels in cotyledons followed the same temporal trend as observed for sucrose and this was reflected in an earlier appearance of protein bodies. Protein levels in wild type and StSUT1 cotyledons were found to lie on the same sucrose dose-response curve and this could be reproduced in vitro when wild type cotyledons were cultured on media containing various sucrose concentrations. Rates of [14C]sucrose uptake and incorporation into polymeric forms were consistent with protoplasmic sucrose supplying a proportion of the carbon skeletons required for storage protein accumulation. In addition, vicilin gene expression was up-regulated earlier in StSUT1 cotyledons. We conclude that sucrose functions both as a signal and fuel to stimulate storage protein accumulation and assembly into protein bodies. An earlier stimulation of storage protein synthesis is considered to largely account for the 14% increase in protein levels of StSUT1 seeds at harvest.

scholarly journals Protein bodies formation in yellow lupin seeds

2014 ◽  
Vol 53 (1) ◽  
pp. 3-9 ◽  
Author(s):  
Adam Woźny ◽  
Fortunat Młodzianowski ◽  
Barbara Stefaniak
Keyword(s):  
Protein Synthesis ◽  
Seed Development ◽  
Storage Protein ◽  
Cell Walls ◽  
Protein Bodies ◽  
Central Vacuole ◽  
Yellow Lupin ◽  
Storage Lipids ◽  
Lupin Seeds ◽  
Cotyledon Cells

The ultrastructure of cotyledon cells is described at five stages of lupin seed development, distinguished on the basis of their morphological features. It was found that the endoplasmic reticulum nad dictyosomes participate in the synthesis and transport of storage protein, and that protein is deposited in the central vacuole or in newly forming ones. In the investigated zone of lupin cotyledon cells two forms of protein bodies were observed differing in the contrast and compactness. Both were the simple protein, bodies. Parallelly to storage protein synthesis, thickening of the cell walls was observed which may indicate synthesis and deposition of hemicellulose in them. Storage lipids surrounding the protein bodies form in the end stages of seed development.

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