scholarly journals Biochemical and Molecular Analysis of Pink Tomatoes: Deregulated Expression of the Gene Encoding Transcription Factor SlMYB12 Leads to Pink Tomato Fruit Color

2009 ◽  
Vol 152 (1) ◽  
pp. 71-84 ◽  
Author(s):  
Ana-Rosa Ballester ◽  
Jos Molthoff ◽  
Ric de Vos ◽  
Bas te Lintel Hekkert ◽  
Diego Orzaez ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Molecular Analysis ◽  
Tomato Fruit ◽  
Fruit Color ◽  
Gene Encoding

A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening

2006 ◽  
Vol 38 (8) ◽  
pp. 948-952 ◽  
Author(s):  
Kenneth Manning ◽  
Mahmut Tör ◽  
Mervin Poole ◽  
Yiguo Hong ◽  
Andrew J Thompson ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fruit Ripening ◽  
Tomato Fruit ◽  
Gene Encoding ◽  
Naturally Occurring ◽  
Tomato Fruit Ripening

scholarly journals Regulation of pepper fruit color, chloroplasts development and their importance in fruit quality

2014 ◽  
Author(s):  
Ilan Paran ◽  
Allen Van Deynze
Keyword(s):  
Transcription Factor ◽  
Candidate Gene ◽  
Chlorophyll Content ◽  
Tomato Fruit ◽  
Fruit Color ◽  
Immature Fruit ◽  
Functional Conservation ◽  
Dna And Rna ◽  
Bulked Dna ◽  
Pepper Fruit

Pepper exhibits large natural variation in chlorophyll content in the immature fruit. To dissect the genetic and molecular basis of this variation, we conducted QTL mapping for chlorophyll content in a cross between light and dark green-fruited parents, PI 152225 and 1154. Two major QTLs, pc1 and pc10, that control chlorophyll content by modulation of chloroplast compartment size in a fruit-specific manner were detected in chromosomes 1 and 10, respectively. The pepper homolog of GOLDEN2- LIKE transcription factor (CaGLK2) was found as underlying pc10, similar to its effect on tomato fruit chloroplast development. A candidate gene for pc1was found as controlling chlorophyll content in pepper by the modulation of chloroplast size and number. Fine mapping of pc1 aided by bulked DNA and RNA-seq analyses enabled the identification of a zinc finger transcription factor LOL1 (LSD-One-Like 1) as a candidate gene underlying pc1. LOL1 is a positive regulator of oxidative stress- induced cell death in Arabidopsis. However, over expression of the rice ortholog resulted in an increase of chlorophyll content. Interestingly, CaAPRR2 that is linked to the QTL and was found to affect immature pepper fruit color in a previous study, did not have a significant effect on chlorophyll content in the present study. Verification of the candidate's function was done by generating CRISPR/Cas9 knockout mutants of the orthologues tomato gene, while its knockout experiment in pepper by genome editing is under progress. Phenotypic similarity as a consequence of disrupting the transcription factor in both pepper and tomato indicated its functional conservation in controlling chlorophyll content in the Solanaceae. A limited sequence diversity study indicated that null mutations in CaLOL1 and its putative interactorCaMIP1 are present in C. chinensebut not in C. annuum. Combinations of mutations in CaLOL1, CaMIP1, CaGLK2 and CaAPRR2 are required for the creation of the extreme variation in chlorophyll content in Capsicum. 

scholarly journals Differential activation mechanisms of two isoforms of Gcr1 transcription factor generated from spliced and un-spliced transcripts in Saccharomyces cerevisiae

2020 ◽  
Author(s):  
Seungwoo Cha ◽  
Chang Pyo Hong ◽  
Hyun Ah Kang ◽  
Ji-Sook Hahn
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcription Factor ◽  
Target Genes ◽  
Gene Encoding ◽  
Metabolic Switch ◽  
Differential Activation ◽  
N Terminus ◽  
Activation Mechanisms ◽  
In Trans ◽  
Separate Activation

Abstract Gcr1, an important transcription factor for glycolytic genes in Saccharomyces cerevisiae, was recently revealed to have two isoforms, Gcr1U and Gcr1S, produced from un-spliced and spliced transcripts, respectively. In this study, by generating strains expressing only Gcr1U or Gcr1S using the CRISPR/Cas9 system, we elucidate differential activation mechanisms of these two isoforms. The Gcr1U monomer forms an active complex with its coactivator Gcr2 homodimer, whereas Gcr1S acts as a homodimer without Gcr2. The USS domain, 55 residues at the N-terminus existing only in Gcr1U, inhibits dimerization of Gcr1U and even acts in trans to inhibit Gcr1S dimerization. The Gcr1S monomer inhibits the metabolic switch from fermentation to respiration by directly binding to the ALD4 promoter, which can be restored by overexpression of the ALD4 gene, encoding a mitochondrial aldehyde dehydrogenase required for ethanol utilization. Gcr1U and Gcr1S regulate almost the same target genes, but show unique activities depending on growth phase, suggesting that these isoforms play differential roles through separate activation mechanisms depending on environmental conditions.

Cloning of the canine gene encoding transcription factor Pit-1 and its exclusion as candidate gene in a canine model of pituitary dwarfism

2000 ◽  
Vol 11 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Irma S. Lantinga-van Leeuwen ◽  
Jan A. Mol ◽  
Hans S. Kooistra ◽  
Ad Rijnberk ◽  
Matthew Breen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Candidate Gene ◽  
Canine Model ◽  
Gene Encoding ◽  
Pituitary Dwarfism

scholarly journals The gene encoding the transcription factor SCIP has features of an expressed retroposon.

1991 ◽  
Vol 11 (9) ◽  
pp. 4642-4650 ◽  
Author(s):  
R Kuhn ◽  
E S Monuki ◽  
G Lemke
Keyword(s):  
Transcription Factor ◽  
Schwann Cells ◽  
Cpg Island ◽  
Single Copy ◽  
Gene Encoding ◽  
Intronless Gene ◽  
Gene Comparison ◽  
Acid Protein ◽  
Central Nervous Systems ◽  
Glial Progenitor Cells

SCIP is a POU domain transcription factor expressed by glial progenitor cells in the peripheral and central nervous systems (dividing Schwann cells and O-2A cells, respectively), where it appears to act as a repressor of myelin-specific genes. We have isolated genomic clones encoding the rat SCIP gene. Comparison of the structure of these clones with genomic Southern blots and SCIP cDNAs demonstrates that SCIP is encoded in a single-copy, intronless gene that has the general features of an expressed retroposon. This gene contributes to an extended CpG island. It is transcribed to produce a 3.1-kb mRNA that encodes a 451-amino-acid protein with a predicted molecular mass of 45 kDa. Immunopurified SCIP antibodies specifically recognize a nuclear protein of this size in cultured proliferating Schwann cells, and gel shift analyses demonstrate that this protein is the predominant octamer-binding protein in these cells.

Autoregulated Expression of Schizosaccharomyces pombe Meiosis-Specific Transcription Factor Mei4 and a Genome-Wide Search for Its Target Genes

Genetics ◽  
2000 ◽  
Vol 154 (4) ◽  
pp. 1497-1508 ◽  
Author(s):  
Hiroko Abe ◽  
Chikashi Shimoda
Keyword(s):  
Transcription Factor ◽  
Schizosaccharomyces Pombe ◽  
Target Genes ◽  
Northern Blotting ◽  
Forkhead Transcription Factor ◽  
Gene Encoding ◽  
Putative Promoter Region ◽  
Cis Acting ◽  
A Genome ◽  
Genome Wide Search

Abstract The Schizosaccharomyces pombe mei4+ gene encoding a forkhead transcription factor is necessary for the progression of meiosis and sporulation. We searched for novel meiotic genes, the expression of which is dependent on Mei4p, since only the spo6+ gene has been assigned to its targets. Six known genes responsible for meiotic recombination were examined by Northern blotting, but none were Mei4 dependent for transcription. We determined the important cis-acting element, designated FLEX, to which Mei4p can bind. The S. pombe genome sequence database (The Sanger Centre, UK) was scanned for the central core heptamer and its flanking 3′ sequence of FLEX composed of 17 nucleotides, and 10 candidate targets of Mei4 were selected. These contained a FLEX-like sequence in the 5′ upstream nontranslatable region within 1 kb of the initiation codon. Northern blotting confirmed that 9 of them, named mde1+ to mde9+, were transcriptionally induced during meiosis and were dependent on mei4+. Most mde genes have not been genetically defined yet, except for mde9+, which is identical to spn5+, which encodes one of the septin family of proteins. mde3+ and a related gene pit1+ encode proteins related to Saccharomyces cerevisiae Ime2. The double disruptant frequently produced asci having an abnormal number and size of spores, although it completed meiosis. We also found that the forkhead DNA-binding domain of Mei4p binds to the FLEX-like element in the putative promoter region of mei4 and that the maximum induction level of mei4 mRNA required functional mei4 activity. Furthermore, expression of a reporter gene driven by the authentic mei4 promoter was induced in vegetative cells by ectopic overproduction of Mei4p. These results suggest that mei4 transcription is positively autoregulated.

Overexpression of the CsFUS3 gene encoding a B3 transcription factor promotes somatic embryogenesis in Citrus

Plant Science ◽  
2018 ◽  
Vol 277 ◽  
pp. 121-131 ◽  
Author(s):  
Zheng Liu ◽  
Xiao-Xia Ge ◽  
Wen-Ming Qiu ◽  
Jian-Mei Long ◽  
Hui-Hui Jia ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Somatic Embryogenesis ◽  
Gene Encoding
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