scholarly journals Multiple Sequence Motifs in the Rubisco Small Subunit Transit Peptide Independently Contribute to Toc159-Dependent Import of Proteins into Chloroplasts

2009 ◽  
Vol 151 (1) ◽  
pp. 129-141 ◽  
Author(s):  
Dong Wook Lee ◽  
Sumin Lee ◽  
Young Jun Oh ◽  
Inhwan Hwang
Keyword(s):  
Transit Peptide ◽  
Small Subunit ◽  
Sequence Motifs ◽  
Multiple Sequence ◽  
Rubisco Small Subunit

scholarly journals Functional Characterization of Sequence Motifs in the Transit Peptide of Arabidopsis Small Subunit of Rubisco

2005 ◽  
Vol 140 (2) ◽  
pp. 466-483 ◽  
Author(s):  
Dong Wook Lee ◽  
Sookjin Lee ◽  
Gil-je Lee ◽  
Kwang Hee Lee ◽  
Sanguk Kim ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Transit Peptide ◽  
Small Subunit ◽  
Sequence Motifs

scholarly journals Identification of a Hsp70 Recognition Domain within the Rubisco Small Subunit Transit Peptide

2000 ◽  
Vol 122 (4) ◽  
pp. 1289-1300 ◽  
Author(s):  
Robert A. Ivey ◽  
Chitra Subramanian ◽  
Barry D. Bruce
Keyword(s):  
Transit Peptide ◽  
Small Subunit ◽  
Rubisco Small Subunit

Multiple functional motifs in the chicken U1 RNA gene enhancer

1987 ◽  
Vol 7 (12) ◽  
pp. 4185-4193
Author(s):  
K A Roebuck ◽  
R J Walker ◽  
W E Stumph
Keyword(s):  
Gene Expression ◽  
Dna Sequences ◽  
Initiation Site ◽  
Sequence Motifs ◽  
Small Nuclear Rna ◽  
Multiple Sequence ◽  
Transcriptional Initiation ◽  
Transcription System ◽  
Cell Extracts ◽  
Gene Enhancer

The DNA sequence requirements of chicken U1 RNA gene expression have been examined in an oocyte transcription system. An enhancer region, which was required for efficient U1 RNA gene expression, is contained within a region of conserved DNA sequences spanning nucleotide positions -230 to -183, upstream of the transcriptional initiation site. These DNA sequences can be divided into at least two distinct subregions or domains that acted synergistically to provide a greater than 20-fold stimulation of U1 RNA synthesis. The first domain contains the octamer sequence ATGCAAAT and was recognized by a DNA-binding factor present in HeLa cell extracts. The second domain (the SPH domain) consists of conserved sequences immediately downstream of the octamer and is an essential component of the enhancer. In the oocyte, the DNA sequences of the SPH domain were able to enhance gene expression at least 10-fold in the absence of the octamer domain. In contrast, the octamer domain, although required for full U1 RNA gene activity, was unable to stimulate expression in the absence of the adjacent downstream DNA sequences. These findings imply that sequences 3' of the octamer play a major role in the function of the chicken U1 RNA gene enhancer. This concept was supported by transcriptional competition studies in which a cloned chicken U4B RNA gene was used to compete for limiting transcription factors in oocytes. Multiple sequence motifs that can function in a variety of cis-linked configurations may be a general feature of vertebrate small nuclear RNA gene enhancers.

Evolutionary History of Plant Lipolytic Enzymes

2018 ◽  
pp. 155-178
Keyword(s):  
Higher Plants ◽  
Evolutionary Relationship ◽  
Biological Evolution ◽  
Sequence Motifs ◽  
Multiple Sequence ◽  
Lipolytic Enzymes ◽  
Conserved Sequence ◽  
Potential Applications ◽  
History Of ◽  
Living Organisms

The discovery of enzymes with lipolytic activities in all kingdoms of life from prokaryote to eukaryote species raises the possibility of the presence of an evolutionary relationship history of these proteins among many species of various living organisms. The chapter suggests a strategy based on the phylogenetic distribution and homology conservation in plant lipolytic enzymes for possible depiction of their biological evolution. Extensive databases and online resources for lipidomics and related areas are useful tools to analyze the different lipolytic enzymes in the three major super kingdoms of life, including higher plants kingdom and confined organisms such as algae that have recently gained much interest due to their promising potential applications in lipids hydrolysis and biosynthesis. Multiple sequence alignments of the identified lipolytic enzymes from databases could serve to the identification of globally conserved residues as well as conserved sequence motifs. Estimation of evolutionary distance between the various identified lipolytic enzymes could also be carried out to better understand the pattern of evolution.

scholarly journals Arabidopsis Nuclear-Encoded Plastid Transit Peptides Contain Multiple Sequence Subgroups with Distinctive Chloroplast-Targeting Sequence Motifs

2008 ◽  
Vol 20 (6) ◽  
pp. 1603-1622 ◽  
Author(s):  
Dong Wook Lee ◽  
Jong Kyoung Kim ◽  
Sumin Lee ◽  
Seungjin Choi ◽  
Sanguk Kim ◽  
...  
Keyword(s):  
Sequence Motifs ◽  
Multiple Sequence ◽  
Chloroplast Targeting ◽  
Transit Peptides
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